Recombinant Anti-PDGFR alpha antibody [EPR5480] (ab134123)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR alpha antibody [EPR5480] (ab134123)](https://www.abcam.com/ps/products/134/ab134123/Images/ab134123-10-anti-pdgfr-alpha-antibody-epr5480-immunohistochemistry-human-colonic-carcinoma.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR alpha antibody [EPR5480] (ab134123)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5480] to PDGFR alpha
- Suitable for: Flow Cyt (Intra), Indirect ELISA, WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-PDGFR alpha antibody [EPR5480]
See all PDGFR alpha primary antibodies -
Description
Rabbit monoclonal [EPR5480] to PDGFR alpha -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), Indirect ELISA, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human colonic carcinoma, human colon tissue. WB: SH SY5Y, C2C12, Human skeletal muscle, Human spleen, and Human fetal brain lysates. Flow Cyt (intra): SH-SY5Y, L6 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5480 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab134123 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/20.
|
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| Indirect ELISA |
Use at an assay dependent concentration.
|
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| WB |
1/1000 - 1/10000. Detects a band of approximately 190 kDa (predicted molecular weight: 122 kDa).
|
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| IHC-P | (4) |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
Flow Cyt (Intra)
1/20. |
|
Indirect ELISA
Use at an assay dependent concentration. |
|
WB
1/1000 - 1/10000. Detects a band of approximately 190 kDa (predicted molecular weight: 122 kDa). |
|
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Receptor that binds both PDGFA and PDGFB and has a tyrosine-protein kinase activity. -
Tissue specificity
Expressed in primary and metastatic colon tumors and in normal colon tissue. Tumors may express a different isoform to that found in normal tissue. -
Involvement in disease
Note=A chromosomal aberration involving PDGFRA is found in some cases of hypereosinophilic syndrome. Interstitial chromosomal deletion del(4)(q12q12) causes the fusion of FIP1L1 and PDGFRA (FIP1L1-PDGFRA). -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 5156 Human
- Entrez Gene: 18595 Mouse
- Entrez Gene: 25267 Rat
- Omim: 173490 Human
- SwissProt: P16234 Human
- SwissProt: P26618 Mouse
- SwissProt: P20786 Rat
- Unigene: 74615 Human
see all -
Alternative names
- Alpha-type platelet-derived growth factor receptor antibody
- CD140 antigen-like family member A antibody
- CD140a antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR alpha antibody [EPR5480] (ab134123)
Immunohistochemical analysis of paraffin-embedded Human colonic carcinoma tissue labeling PDGFR alpha with ab134123 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on stromal cells in human colonic carcinoma. The section was incubated with ab134123 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
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![Western blot - Anti-PDGFR alpha antibody [EPR5480] (ab134123)](https://www.abcam.com/ps/products/134/ab134123/Images/ab134123-456193-anti-pdgfr-alpha-antibody-epr5480-western-blot-wildtype-shsy5y-pdgfra-knockout-shsy5y.jpg)
Western blot - Anti-PDGFR alpha antibody [EPR5480] (ab134123)All lanes : Anti-PDGFR alpha antibody [EPR5480] (ab134123) at 1/1000 dilution
Lane 1 : Wild-type SH-SY5Y cell lysate
Lane 2 : PDGFRA knockout SH-SY5Y cell lysate
Lysates/proteins at 40 µg per lane.
Performed under reducing conditions.
Predicted band size: 122 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?Lanes 1 - 2: Merged signal (red and green). Green - ab134123 observed at 180 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.
ab134123 was shown to react with PDGFR alpha in wild-type SH-SY5Y cells in Western blot with loss of signal observed in PDGFRA knockout cell line ab275335 (knockout cell lysate ab275522). Wild-type SH-SY5Y and PDGFRA knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab134123 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
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![Flow Cytometry (Intracellular) - Anti-PDGFR alpha antibody [EPR5480] (ab134123)](https://www.abcam.com/ps/products/134/ab134123/Images/ab134123-330900-anti-pdgfr-alpha-antibody-epr5480-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-PDGFR alpha antibody [EPR5480] (ab134123)Intracellular Flow Cytometry analysis of L6 (Rat skeletal muscle myoblast) cells labelling PDGFR alpha with ab134123 at 1/20 dilution (14.65 µg/ml) (Red). Cells were fixed with 4% paraformaldehyde . Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlabeled control - Unlabelled cells (Blue).
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![Western blot - Anti-PDGFR alpha antibody [EPR5480] (ab134123)](https://www.abcam.com/ps/products/134/ab134123/Images/ab134123-148340-anti-pdgfr-alpha-antibody-epr5480-western-blot.jpg)
Western blot - Anti-PDGFR alpha antibody [EPR5480] (ab134123)All lanes : Anti-PDGFR alpha antibody [EPR5480] (ab134123) at 1/1000 dilution
Lane 1 : Human skeletal muscle lysate
Lane 2 : Human spleen lysate
Lane 3 : Human fetal brain lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 122 kDa
Observed band size: 190 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR alpha antibody [EPR5480] (ab134123)](https://www.abcam.com/ps/products/134/ab134123/Images/ab134123-9-anti-pdgfr-alpha-antibody-epr5480-immunohistochemistry-human-colon.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR alpha antibody [EPR5480] (ab134123)Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling PDGFR alpha with ab134123 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on stromal cells in human colon (PMID: 23429582). The section was incubated with ab134123 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
-
![Flow Cytometry (Intracellular) - Anti-PDGFR alpha antibody [EPR5480] (ab134123)](https://www.abcam.com/ps/products/134/ab134123/Images/ab134123-330899-anti-pdgfr-alpha-antibody-epr5480-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-PDGFR alpha antibody [EPR5480] (ab134123)Intracellular Flow Cytometry analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labelling PDGFR alpha with ab134123 at 1/20 dilution (14.65µg/ml) (Red). Cells were fixed with 4% paraformaldehyde . Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlabeled control - Unlabelled cells (Blue).
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![Indirect ELISA - Anti-PDGFR alpha antibody [EPR5480] (ab134123)](https://www.abcam.com/ps/products/134/ab134123/Images/ab134123-11-anti-pdgfr-alpha-antibody-epr5480-elisa-human-pdgfr-alpha.jpg)
Indirect ELISA - Anti-PDGFR alpha antibody [EPR5480] (ab134123)ELISA analysis of Human PDGFR alpha recombinant protein at 250 ng/mL with ab134123. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
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![Western blot - Anti-PDGFR alpha antibody [EPR5480] (ab134123)](https://www.abcam.com/ps/products/134/ab134123/Images/ab134123-148341-anti-pdgfr-alpha-antibody-epr5480-western-blot.jpg)
Western blot - Anti-PDGFR alpha antibody [EPR5480] (ab134123)All lanes : Anti-PDGFR alpha antibody [EPR5480] (ab134123) at 1/1000 dilution
Lane 1 : SH-SY5Y (human neuroblastoma cell line from bone marrow) cell lysate
Lane 2 : C2C12 (mouse myoblast cell line) cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 122 kDa
Observed band size: 190 kDa why is the actual band size different from the predicted? -
![Anti-PDGFR alpha antibody [EPR5480] (ab134123)](https://www.abcam.com/ps/products/134/ab134123/Images/ab134123-7-benefits-of-recombinant-antibodies.png)
Anti-PDGFR alpha antibody [EPR5480] (ab134123)
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab134123 has been referenced in 4 publications.
- Du Y et al. The Epithelial to Mesenchymal Transition Related Gene Calumenin Is an Adverse Prognostic Factor of Bladder Cancer Correlated With Tumor Microenvironment Remodeling, Gene Mutation, and Ferroptosis. Front Oncol 11:683951 (2021). PubMed: 34150647
- Landry NM et al. An Improved Method of Maintaining Primary Murine Cardiac Fibroblasts in Two-Dimensional Cell Culture. Sci Rep 9:12889 (2019). PubMed: 31501457
- Wang Y et al. Necrostatin-1 ameliorates the pathogenesis of experimental autoimmune encephalomyelitis by suppressing apoptosis and necroptosis of oligodendrocyte precursor cells. Exp Ther Med 18:4113-4119 (2019). PubMed: 31611942
- Schmid FM et al. IFT20 modulates ciliary PDGFRa signaling by regulating the stability of Cbl E3 ubiquitin ligases. J Cell Biol 217:151-161 (2018). PubMed: 29237719