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Validated using a knockout cell line

Anti-PDHA1 antibody [8D10E6] (ab110334)

Reviews (6)Q&A (5)References (60)
Western blot - Anti-PDHA1 antibody [8D10E6] (ab110334)
  • Western blot - Anti-PDHA1 antibody [8D10E6] (ab110334)
  • Immunocytochemistry/ Immunofluorescence - Anti-PDHA1 antibody [8D10E6] (ab110334)
  • Flow Cytometry - Anti-PDHA1 antibody [8D10E6] (ab110334)

Key features and details

  • Mouse monoclonal [8D10E6] to PDHA1
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Knockout validated
  • Reacts with: Mouse, Rat, Cow, Human, Drosophila melanogaster
  • Isotype: IgG1

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 HRP

Overview

  • Product name

    Anti-PDHA1 antibody [8D10E6]
    See all PDHA1 primary antibodies

  • Description

    Mouse monoclonal [8D10E6] to PDHA1

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Cow, Human, Drosophila melanogaster
    Predicted to work with: Zebrafish

  • Immunogen

    Full length native protein (purified). This information is considered to be commercially sensitive.

  • Positive control

    • WB: Isolated mitochondria from human, bovine, rat and mouse heart. HepG2 (human liver hepatocellular carcinoma cell line) cell lysate. FACS: HeLa (human epithelial cell line from cervix adenocarcinoma) and HL-60 (human promyelocytic leukemia cell line) cells.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Product was previously marketed under the MitoSciences sub-brand.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab110334 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (6)
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 43 kDa.
ICC/IF
Use a concentration of 1 µg/ml.

(heat-induced antigen-retrieval improves signal)
Flow Cyt
Use a concentration of 1 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Notes
WB
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 43 kDa.
ICC/IF
Use a concentration of 1 µg/ml.

(heat-induced antigen-retrieval improves signal)
Flow Cyt
Use a concentration of 1 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function

    The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).

  • Tissue specificity

    Ubiquitous.

  • Involvement in disease

    Defects in PDHA1 are a cause of pyruvate decarboxylase E1 component deficiency (PDHE1 deficiency) [MIM:312170]. PDHE1 deficiency is the most common enzyme defect in patients with primary lactic acidosis. It is associated with variable clinical phenotypes ranging from neonatal death to prolonged survival complicated by developmental delay, seizures, ataxia, apnea, and in some cases to an X-linked form of Leigh syndrome (X-LS).
    Defects in PDHA1 are the cause of X-linked Leigh syndrome (X-LS) [MIM:308930]. X-LS is an early-onset progressive neurodegenerative disorder with a characteristic neuropathology consisting of focal, bilateral lesions in one or more areas of the central nervous system, including the brainstem, thalamus, basal ganglia, cerebellum, and spinal cord. The lesions are areas of demyelination, gliosis, necrosis, spongiosis, or capillary proliferation. Clinical symptoms depend on which areas of the central nervous system are involved. The most common underlying cause is a defect in oxidative phosphorylation. LS may be a feature of a deficiency of any of the mitochondrial respiratory chain complexes.

  • Cellular localization

    Mitochondrion matrix.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • ODPA_HUMAN antibody
    • PDH antibody
    • PDHA antibody
    • PDHA1 antibody
    • PDHCE1A antibody
    • PDHE1 A type I antibody
    • PDHE1-A type I antibody
    • PHE1A antibody
    • Pyruvate Dehydrogenase (lipoamide) alpha 1 antibody
    • Pyruvate dehydrogenase complex, E1 alpha polypeptide 1 antibody
    • Pyruvate Dehydrogenase E1 alpha antibody
    • Pyruvate dehydrogenase E1 component subunit alpha antibody
    • Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial antibody
    • Pyruvate Dehydrogenase E1-alpha subunit antibody
    see all

Images

  • Western blot - Anti-PDHA1 antibody [8D10E6] (ab110334)
    Western blot - Anti-PDHA1 antibody [8D10E6] (ab110334)
    All lanes : Anti-PDHA1 antibody [8D10E6] (ab110334) at 0.5 µg/ml

    Lane 1 : Wild-type HeLa whole cell lysate
    Lane 2 : PDHA1 knockout HeLa whole cell lysate
    Lane 3 : HEK-293 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 43 kDa
    Observed band size: 43 kDa



    Lanes 1 - 3: Merged signal (red and green). Green - ab110334 observed at 43 kDa. Red - loading control, ab52866, observed at 50 kDa.

    ab110334 was shown to recognize PDHA1 in wild-type HeLa cells as signal was lost at the expected MW in PDHA1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and PDHA1 knockout samples were subjected to SDS-PAGE. Ab110334 and ab52866 (Rabbit anti alpha Tubulin loading control) were incubated overnight at 4°C at 0.5 ug/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-PDHA1 antibody [8D10E6] (ab110334)
    Western blot - Anti-PDHA1 antibody [8D10E6] (ab110334)
    All lanes : Anti-PDHA1 antibody [8D10E6] (ab110334) at 1 µg/ml

    Lane 1 : Isolated mitochondria from human heart at 5 µg
    Lane 2 : Isolated mitochondria from bovine heart at 1 µg
    Lane 3 : Isolated mitochondria from rat heart at 10 µg
    Lane 4 : Isolated mitochondria from mouse heart at 10 µg
    Lane 5 : HepG2 cell lysate at 20 µg

    Predicted band size: 43 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-PDHA1 antibody [8D10E6] (ab110334)
    Immunocytochemistry/ Immunofluorescence - Anti-PDHA1 antibody [8D10E6] (ab110334)

    Immunocytochemistry analysis using ab110334 at 1 µg/ml staining PDHA1 in HeLa (human epithelial cell line from cervix adenocarcinoma) cells (4% paraformaldehyde fixed and Triton X-100 permeabilized).
    The secondary antibody was (green) Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution. DAPI was used to stain the cell nuclei (blue).

  • Flow Cytometry - Anti-PDHA1 antibody [8D10E6] (ab110334)
    Flow Cytometry - Anti-PDHA1 antibody [8D10E6] (ab110334)

    Flow cytometric analysis using ab110334 at 1 µg/ml staining PDHA1 in HL-60 (human promyelocytic leukemia cell line) cells (blue). Isotype control antibody (red).

References (60)

Publishing research using ab110334? Please let us know so that we can cite the reference in this datasheet.

ab110334 has been referenced in 60 publications.

  • Duraj T  et al. Beyond the Warburg Effect: Oxidative and Glycolytic Phenotypes Coexist within the Metabolic Heterogeneity of Glioblastoma. Cells 10:N/A (2021). PubMed: 33498369
  • Yuan Q  et al. Inhibition of mitochondrial carrier homolog 2 (MTCH2) suppresses tumor invasion and enhances sensitivity to temozolomide in malignant glioma. Mol Med 27:7 (2021). PubMed: 33509092
  • Cenigaonandia-Campillo A  et al. Vitamin C activates pyruvate dehydrogenase (PDH) targeting the mitochondrial tricarboxylic acid (TCA) cycle in hypoxic KRAS mutant colon cancer. Theranostics 11:3595-3606 (2021). PubMed: 33664850
  • Fiorentino TV  et al. Pioglitazone corrects dysregulation of skeletal muscle mitochondrial proteins involved in ATP synthesis in type 2 diabetes. Metabolism 114:154416 (2021). PubMed: 33137378
  • Tian L  et al. Supra-coronary aortic banding improves right ventricular function in experimental pulmonary arterial hypertension in rats by increasing systolic right coronary artery perfusion. Acta Physiol (Oxf) 229:e13483 (2020). PubMed: 32339403
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-10 of 11 Abreviews or Q&A

Western blot abreview for Anti-Pyruvate Dehydrogenase E1-alpha subunit antibody [8D10E6]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Sheep Tissue lysate - whole (Skeletal Muscle)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
50 µg
Specification
Skeletal Muscle
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Alex Pendleton

Verified customer

Submitted Nov 14 2017

Western blot abreview for Anti-Pyruvate Dehydrogenase E1-alpha subunit antibody [8D10E6]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (Retina)
Gel Running Conditions
Reduced Denaturing (12% gel)
Loading amount
20 µg
Specification
Retina
Blocking step
Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Aug 01 2017

Western blot abreview for Anti-Pyruvate Dehydrogenase E1-alpha subunit antibody [8D10E6]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK cells)
Gel Running Conditions
Reduced Denaturing (12% gel)
Loading amount
20 µg
Specification
HEK cells
Blocking step
Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 03 2017

Western blot abreview for Anti-Pyruvate Dehydrogenase E1-alpha subunit antibody [8D10E6]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver, retina)
Gel Running Conditions
Reduced Denaturing (12% gel)
Loading amount
20 µg
Specification
Liver, retina
Blocking step
Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 03 2017

Western blot abreview for Anti-Pyruvate Dehydrogenase E1-alpha subunit antibody [8D10E6]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Zebrafish Tissue lysate - whole (Retina)
Gel Running Conditions
Reduced Denaturing (12% gel)
Loading amount
20 µg
Specification
Retina
Blocking step
Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 03 2017

Western blot abreview for Anti-Pyruvate Dehydrogenase E1-alpha subunit antibody [8D10E6]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Euglena Cell lysate - whole cell (Euglena gracilis SM-ZK)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
15 µg
Treatment
silencing of pyruvate dehydrogenase E1-alpha subunit or not
Specification
Euglena gracilis SM-ZK
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jul 07 2016

Question

I would be interested in the offer of a discount after supplying an abreview. But I am just wondering if there is a possibility of getting a sample of this antibody first? As our lab has found in the past that some antibodies even if the sequence is quite homologous it does not always work and so I would be cautious about buying this antibody before it has been tested.

Kind Regards,

Read More
Answer

Thank you for contacting us.

We unfortunately do not provide free samples, this is why we have 100% Abtrial offer. If this antibody does not work you will still be getting a free antibody with Abtrial code.

Please let me know if you are interested in getting this code.

Thanks!

Read More

Question

Inquiry: Is it known whether this antibody works with horse samples? I am working with skeletal muscle isolated mitochondria and I am looking for a loading control antibody for western blotting. I have been using Anti-Pyruvate Dehydrogenase E1-alpha subunit antibody [9H9AF5 ] (ab110330) when using rat and mouse samples but I now need one that will work with horse also.

Read More
Answer

Thank you for contacting us.

This product ab110330 was tested with Mouse, Rat, Cow and Human samples only; horse was never tried. We can however predict, it will be fine for horse samples as well because the horse protein (http://www.uniprot.org/uniprot/F6WQM7) is quite homologous to the human protein (http://www.uniprot.org/uniprot/P08559).

In case you are interested in trying this antibody then I would encourage you to participate in our 100% Abtrial discount programme - a free product can be claimed in exchange of review for this antibody. Please contact me for code if you accept our offer.

More details about the offer can be found at the following link;

https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Question

One person in our lab has two antibodies not working now:
" 1. anti-Pyruvate Dehydrogenase (Mouse monoclonal): Abcam (Catalog#
ab110334). Stored at 4 degrees with our other
antibodies.
2. anti-SDHA (Mouse monoclonal): Abcam (Catalog# ab14715).
Stored at 4 degrees with our other antibodies. Our other antibodies
(even those ordered much before than these) are working fine. I used
these antibodies 4 times for SDHA and 3 times for PDH on different
dates. So the probability of them being outside for prolonged period
and other physical insults is negligible. I think I am the only one in
the lab who uses these antibodies. so mishandling is also not an
issue."
Both of them worked in the beginning beautifully but when I revisited
them, they were not working.
Would you please help me with that? Please let me know if you have any
questions, thank you.

Read More
Answer

Thank you for contacting us. I am sorry that these antibodies are no longer working properly. I will be happy to help you resolve this issue if you can please provide some additional details about your experiment?

1.) What application were you using these antibodies in (WB, IHC-P, etc)?

2.) What species, tissue type, or cell line were you testing? How were the samples prepared (type of buffer used for lysis, fixation, permeabilization, embedding method, etc)?

3.) What blocking agent was used and at what concentration?

4.) What were the primary and secondary antibody dilutions and incubation times?

5.) Could you please provide a description of the problem (no signal, high background, etc) and / or an image of your results?

6.) What was your original order or PO number?

I will be happy to help you further once I have this additional information.

Read More

Question

One person in our lab has two antibodies not
working now:
" 1. anti-Pyruvate Dehydrogenase (Mouse monoclonal): Abcam (Catalog#
ab110334) Lot# GR47055-1. Stored at 4 degrees with our other
antibodies.
2. anti-SDHA (Mouse monoclonal): Abcam (Catalog# ab14715) Lot# 888711.
Stored at 4 degrees with our other antibodies. Our other antibodies
(even those ordered much before than these) are working fine. I used
these antibodies 4 times for SDHA and 3 times for PDH on different
dates. So the probability of them being outside for prolonged period
and other physical insults is negligible. I think I am the only one in
the lab who uses these antibodies. so mishandling is also not an
issue."
Both of them worked in the beginning beautifully but when I revisited
them, they were not working.
Would you please help me with that? Please let me know if you have any
questions, thank you.

Read More
Answer

Thank you for contacting us.

I found the original order for these ordered on July 28 2011. Since these antibodies are recommended to be stored at 4C, they only have a shelf-life of roughly 1 yr. If the end-user is seeing decreased signal and the antibodies worked well in the past they may have to increase the working dilution and/or incubation time.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

1-10 of 11 Abreviews or Q&A

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