Anti-PDHA1 antibody [9H9AF5] (ab110330)
![Western blot - Anti-PDHA1 antibody [9H9AF5] (ab110330)](https://www.abcam.com/ps/products/110/ab110330/Images/ab110330-349200-anti-pyruvatedehydrogenase-e1-alpha-subunit-antibody-9h9af5-westernblot-knock-out-hela-pdha1-hek293-human-lysates.jpg "Western blot - Anti-PDHA1 antibody [9H9AF5] (ab110330)")
Key features and details
- Mouse monoclonal [9H9AF5] to PDHA1
- Suitable for: WB, ICC/IF, Flow Cyt
- Knockout validated
- Reacts with: Mouse, Rat, Cow, Human
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
Anti-PDHA1 antibody [9H9AF5]
See all PDHA1 primary antibodies -
Description
Mouse monoclonal [9H9AF5] to PDHA1 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Rat, Cow, Human -
Immunogen
Recombinant full length protein corresponding to Human PDHA1.
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Positive control
- Isolated mitochondria from Human, Bovine, Rat and Mouse hearts, HepG2 lysate; cultured, normal Human embryonic lung fibroblasts (strain MRC5); HL60 cells.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
Purity
IgG fraction -
Purification notes
ab110330 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
Clonality
Monoclonal -
Clone number
9H9AF5 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab110330 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (6) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 43 kDa.
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| ICC/IF | (1) |
Use a concentration of 5 µg/ml.
(heat-induced antigen-retrieval improves signal) |
| Flow Cyt |
Use a concentration of 1 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 43 kDa. |
|
ICC/IF
Use a concentration of 5 µg/ml. (heat-induced antigen-retrieval improves signal) |
|
Flow Cyt
Use a concentration of 1 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). -
Tissue specificity
Ubiquitous. -
Involvement in disease
Defects in PDHA1 are a cause of pyruvate decarboxylase E1 component deficiency (PDHE1 deficiency) [MIM:312170]. PDHE1 deficiency is the most common enzyme defect in patients with primary lactic acidosis. It is associated with variable clinical phenotypes ranging from neonatal death to prolonged survival complicated by developmental delay, seizures, ataxia, apnea, and in some cases to an X-linked form of Leigh syndrome (X-LS).
Defects in PDHA1 are the cause of X-linked Leigh syndrome (X-LS) [MIM:308930]. X-LS is an early-onset progressive neurodegenerative disorder with a characteristic neuropathology consisting of focal, bilateral lesions in one or more areas of the central nervous system, including the brainstem, thalamus, basal ganglia, cerebellum, and spinal cord. The lesions are areas of demyelination, gliosis, necrosis, spongiosis, or capillary proliferation. Clinical symptoms depend on which areas of the central nervous system are involved. The most common underlying cause is a defect in oxidative phosphorylation. LS may be a feature of a deficiency of any of the mitochondrial respiratory chain complexes. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
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Database links
- Entrez Gene: 407109 Cow
- Entrez Gene: 5160 Human
- Entrez Gene: 18597 Mouse
- Entrez Gene: 29554 Rat
- Omim: 300502 Human
- SwissProt: A7MB35 Cow
- SwissProt: P08559 Human
- SwissProt: P35486 Mouse
see all -
Alternative names
- ODPA_HUMAN antibody
- PDH antibody
- PDHA antibody
see all
Images
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Western blot - Anti-PDHA1 antibody [9H9AF5] (ab110330)All lanes : Anti-PDHA1 antibody [9H9AF5] (ab110330) at 1 µg/ml
Lane 1 : Wild-type HeLa whole cell lysate
Lane 2 : PDHA1 knockout HeLa whole cell lysate
Lane 3 : HEK-293 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 43 kDa
Observed band size: 43 kDaLanes 1 - 3: Merged signal (red and green). Green - ab110330 observed at 43 kDa. Red - loading control, ab52866, observed at 50 kDa.
ab110330 was shown to specifically react with PDHA1 in wild-type HeLa cells as signal was lost in PDHA1 knockout cells. Wild-type and PDHA1 knockout samples were subjected to SDS-PAGE. Ab110330 and ab52866 (Rabbit anti alpha Tubulin loading control) were incubated overnight at 4°C at 1 ug/ml and 1/1000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-PDHA1 antibody [9H9AF5] (ab110330)](https://www.abcam.com/ps/products/110/ab110330/Images/ab110330-123644-anti-pyruvate-dehydrogenase-e1-alpha-subunit-antibody-9h9af5-western-blot.jpg)
Western blot - Anti-PDHA1 antibody [9H9AF5] (ab110330)All lanes : Anti-PDHA1 antibody [9H9AF5] (ab110330) at 1 µg/ml
Lane 1 : Isolated mitochondria from Human heart at 10 µg
Lane 2 : Isolated mitochondria from Bovine heart at 4 µg
Lane 3 : Isolated mitochondria from Rat heart at 10 µg
Lane 4 : Isolated mitochondria from Mouse heart at 10 µg
Lane 5 : hepG2 cell lysate at 20 µg
Predicted band size: 43 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-PDHA1 antibody [9H9AF5] (ab110330)](https://www.abcam.com/ps/products/110/ab110330/Images/ab110330-123643-anti-pyruvate-dehydrogenase-e1-alpha-subunit-antibody-9h9af5-immunofluorescence.gif)
Immunocytochemistry/ Immunofluorescence - Anti-PDHA1 antibody [9H9AF5] (ab110330)Immunocytochemistry analysis using ab110330 at 5µg/ml staining PDHA1 in cultured, normal Human embryonic lung fibroblasts (strain MRC5) followed by AlexaFluor® 488 goat anti-mouse IgG1 secondary antibody (2 µg/ml).
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![Immunocytochemistry/ Immunofluorescence - Anti-PDHA1 antibody [9H9AF5] (ab110330)](https://www.abcam.com/ps/products/110/ab110330/Images/ab110330-261069-anti-pyruvate-dehydrogenase-e1-alpha-subunit-antibody-9h9af5-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PDHA1 antibody [9H9AF5] (ab110330)'This image is courtesy of an Abreview submitted by George Allen'Immunocytochemical analysis of SH-SY5Y human neuroblastoma labeling PDHA1 with ab110330 at 1/500 dilution.
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![Flow Cytometry - Anti-PDHA1 antibody [9H9AF5] (ab110330)](https://www.abcam.com/ps/products/110/ab110330/Images/ab110330-123642-anti-pyruvate-dehydrogenase-e1-alpha-subunit-antibody-9h9af5-flow-cytometry.gif)
Flow Cytometry - Anti-PDHA1 antibody [9H9AF5] (ab110330)Flow cytometric analysis using ab110330 at 1µg/ml staining PDHA1 in HL60 cells (blue). Isotype control antibody (red).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (69)
ab110330 has been referenced in 69 publications.
- Kim CJ et al. Cryptotanshinone, a novel PDK 4 inhibitor, suppresses bladder cancer cell invasiveness via the mTOR/ß-catenin/N-cadherin axis. Int J Oncol 59:N/A (2021). PubMed: 33982789
- Todkar K et al. Selective packaging of mitochondrial proteins into extracellular vesicles prevents the release of mitochondrial DAMPs. Nat Commun 12:1971 (2021). PubMed: 33785738
- Sabui S et al. Developmental maturation of the colonic uptake process of the microbiota-generated thiamin pyrophosphate. Am J Physiol Gastrointest Liver Physiol 320:G829-G835 (2021). PubMed: 33759569
- Roca-Agujetas V et al. Cholesterol alters mitophagy by impairing optineurin recruitment and lysosomal clearance in Alzheimer's disease. Mol Neurodegener 16:15 (2021). PubMed: 33685483
- Singulani MP et al. Impairment of PGC-1a-mediated mitochondrial biogenesis precedes mitochondrial dysfunction and Alzheimer's pathology in the 3xTg mouse model of Alzheimer's disease. Exp Gerontol 133:110882 (2020). PubMed: 32084533