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Anti-PDHB antibody [17A5E2H8] (ab110331)

Reviews (1)Q&A (3)References (5)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHB antibody [17A5E2H8] (ab110331)
  • Western blot - Anti-PDHB antibody [17A5E2H8] (ab110331)

Key features and details

  • Mouse monoclonal [17A5E2H8] to PDHB
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Cow, Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-PDHB antibody [17A5E2H8]
    See all PDHB primary antibodies

  • Description

    Mouse monoclonal [17A5E2H8] to PDHB

  • Host species

    Mouse

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Cow, Human

  • Immunogen

    Full length protein. This information is considered to be commercially sensitive.

  • Positive control

    • Isolated mitochondria from Human, Bovine, Rat and Mouse hearts; HepG2 whole cell lysate (ab7900). This antibody gave a positive result in IHC in the following FFPE tissue: Human normal duodenum.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Product was previously marketed under the MitoSciences sub-brand.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab110331 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB (1)
Use a concentration of 1 µg/ml. Predicted molecular weight: 39 kDa.
Notes
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 39 kDa.

Target

  • Function

    The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).

  • Involvement in disease

    Defects in PDHB are a cause of pyruvate dehydrogenase E1 component deficiency (PDHE1 deficiency) [MIM:312170]. PDHE1 deficiency is the most common enzyme defect in patients with primary lactic acidosis. It is associated with variable clinical phenotypes ranging from neonatal death to prolonged survival complicated by developmental delay, seizures, ataxia, apnea, and in some cases to an X-linked form of Leigh syndrome (LS) (Leigh encephalomyelopathy).

  • Cellular localization

    Mitochondrion matrix.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • DKFZp564K0164 antibody
    • mitochondrial antibody
    • ODPB_HUMAN antibody
    • pdhB antibody
    • PDHBD antibody
    • PDHE1 B antibody
    • PDHE1-B antibody
    • PHE1B antibody
    • Pyruvate dehydrogenase (lipoamide) beta antibody
    • Pyruvate dehydrogenase E1 beta polypeptide antibody
    • Pyruvate dehydrogenase E1 component subunit beta antibody
    • Pyruvate dehydrogenase E1 component subunit beta mitochondrial antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHB antibody [17A5E2H8] (ab110331)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHB antibody [17A5E2H8] (ab110331)

    IHC image of PDHB staining in Human normal duodenum formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab110331, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Western blot - Anti-PDHB antibody [17A5E2H8] (ab110331)
    Western blot - Anti-PDHB antibody [17A5E2H8] (ab110331)
    All lanes : Anti-PDHB antibody [17A5E2H8] (ab110331) at 1 µg/ml

    Lane 1 : Isolated mitochondria from Human heart at 10 µg/ml
    Lane 2 : Isolated mitochondria from Bovine heart at 4 µg/ml
    Lane 3 : Isolated mitochondria from Rat heart at 10 µg/ml
    Lane 4 : Isolated mitochondria from Mouse heart at 10 µg/ml
    Lane 5 : HepG2 cell lysate at 20 µg/ml

    Predicted band size: 39 kDa

References (5)

Publishing research using ab110331? Please let us know so that we can cite the reference in this datasheet.

ab110331 has been referenced in 5 publications.

  • Kam JH  et al. Mitochondrial absorption of short wavelength light drives primate blue retinal cones into glycolysis which may increase their pace of aging. Vis Neurosci 36:E007 (2019). PubMed: 31199213
  • Qi X  et al. Knockdown of prohibitin expression promotes glucose metabolism in eutopic endometrial stromal cells from women with endometriosis. Reprod Biomed Online 29:761-70 (2014). PubMed: 25444511
  • Halim ND  et al. Phosphorylation status of pyruvate dehydrogenase distinguishes metabolic phenotypes of cultured rat brain astrocytes and neurons. Glia 58:1168-76 (2010). PubMed: 20544852
  • Han Z  et al. A combined therapeutic approach for pyruvate dehydrogenase deficiency using self-complementary adeno-associated virus serotype-specific vectors and dichloroacetate. Mol Genet Metab 93:381-7 (2008). PubMed: 18206410
  • Arcelay E  et al. Identification of proteins undergoing tyrosine phosphorylation during mouse sperm capacitation. Int J Dev Biol 52:463-72 (2008). PubMed: 18649259

Customer reviews and Q&As

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1-4 of 4 Abreviews or Q&A

Western blot abreview for Anti-PDHB antibody [17A5E2H8]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - other (keratinocytes mitochondria fraction)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
66 µg
Specification
keratinocytes mitochondria fraction
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 26 2013

Question

I could use either of the antibodies made from the recombinant fragment or synthetic peptide and I would still see a band? Because I want to confirm that only the specific epitope site has been altered and none others. I guess I don't understand how the starting material (recombinant fragment or synthetic peptide) affects binding to the antigen site. I would assume that a shorter, specific clone would only bind to the complimentary antigen site rather than the whole protein. Am I thinking about this correctly? Sorry for the stream of consciousness...

Read More
Answer

Thank you for your reply.
As long as your mutant construct contains the either amino acid regions 72-121 or 250-360, then you should be able to either ab84588 orab55574, as these antibodies will bind within these regions respectively.
Whether you use a recombinant protein or synthetic peptide generally will not be an issue, as a monoclonal antibody will only bind to one site regardless of what method you use to generate the antibody. Whereas a polyclonal will bind to multiply sites, all within the region that was used as the immunogen.
I hope that make sense and helps.
Please let me know if there is anything else I can help you with.

Read More

Question

I need some assistance selecting an antibody. I have created a PDHB mutant that I would like to test additionally via western blot. I used ab110331 and there was no band on the gel (a control indicated that the blot worked successfully). However, I’d like to test this mutant further. Since I altered the amino acid sequence towards the end of the gene, I’m wondering if I can confirm that other epitopes have or haven’t been altered. Are the other antibodies for PDHB targeted to other epitope sites? What about the antibodies created off shorter recombinant fragments (e.g. ab55574 or ab84588)?

Read More
Answer

Thank you for contacting Abcam.
Depending on the exact amino acids that are missing from your mutant construct, one of the Pyruvate Dehydrogenase E1 beta subunit antibodies we have should work for you. Below is the immunogen information for each of the antibodies you mentioned:
- ab110331 - Isolated mitochondria from Human, Bovine, Rat and Mouse hearts; HepG2 cell lysate. The exact epitope that this antibody recognizes has not been mapped and so we do not know where it binds.
- ab55574 - Recombinant fragment, corresponding to amino acids 250-360 of Human Pyruvate Dehydrogenase E1 beta subunit
- ab84588 - Synthetic peptide corresponding to a region within N terminal amino acids 72-121 (GLWKKYGDKR IIDTPISEMG FAGIAVGAAM AGLRPICEFM TFNFSMQAID) of Human Pyruvate Dehydrogenase E1 beta subunit.
If there is anything else I can help you with, please let me know.

Read More

Question

Very weak signal in western blot when using human cell lines.

Read More
Answer

Thank you for calling Abcam.

I am sorry that you were having issues with ab110331 in western blot. As requested I have sent you ab55574 as a free of charge replacement, your new order number is ********.

Please let me know if there is anything else I can help you with.

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