Recombinant Anti-PDLIM1/CLP36 antibody [EPR7187] (ab126628)
![Western blot - Anti-PDLIM1/CLP36 antibody [EPR7187] (ab126628)](https://www.abcam.com/ps/products/126/ab126628/Images/ab126628-140631-anti-pdlim1-antibody-epr7187-western-blot.jpg "Western blot - Anti-PDLIM1/CLP36 antibody [EPR7187] (ab126628)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7187] to PDLIM1/CLP36
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-PDLIM1/CLP36 antibody [EPR7187]
See all PDLIM1/CLP36 primary antibodies -
Description
Rabbit monoclonal [EPR7187] to PDLIM1/CLP36 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide within Human PDLIM1/CLP36 aa 200-300. The exact sequence is proprietary.
Database link: O00151 -
Positive control
- Jurkat cell line lysate HeLa cell line lysate and cells 293T cell line lysate Caco-2 cell line lysate Saos-2 cell line lysate
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7187 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab126628 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 36 kDa.
|
|
| ICC/IF |
1/250 - 1/500.
|
| Notes |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 36 kDa. |
|
ICC/IF
1/250 - 1/500. |
Application notes
Is unsuitable for IHC-P or IP.
Target
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Function
Cytoskeletal protein that may act as an adapter that brings other proteins (like kinases) to the cytoskeleton. -
Tissue specificity
Strongly expressed in the heart and skeletal muscle, moderately expressed in the spleen, small intestine, colon, placenta, and lung. A lower level expression is seen in liver, thymus, kidney, prostate and pancreas and is not found in the brain, testis, ovary, and peripheral blood leukocytes. -
Sequence similarities
Contains 1 LIM zinc-binding domain.
Contains 1 PDZ (DHR) domain. -
Cellular localization
Cytoplasm. Cytoplasm > cytoskeleton. Associates with the actin stress fibers. - Information by UniProt
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Database links
- Entrez Gene: 9124 Human
- Entrez Gene: 54132 Mouse
- Entrez Gene: 54133 Rat
- Omim: 605900 Human
- SwissProt: O00151 Human
- SwissProt: O70400 Mouse
- SwissProt: Q3TZ17 Mouse
- SwissProt: P52944 Rat
see all -
Alternative names
- C terminal LIM domain protein 1 antibody
- C-terminal LIM domain protein 1 antibody
- Carboxy terminal LIM domain protein 1 antibody
see all
Images
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Western blot - Anti-PDLIM1/CLP36 antibody [EPR7187] (ab126628)All lanes : Anti-PDLIM1/CLP36 antibody [EPR7187] (ab126628) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293T cell lysate
Lane 4 : Caco 2 cell lysate
Lane 5 : Saos 2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 36 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-PDLIM1/CLP36 antibody [EPR7187] (ab126628)](https://www.abcam.com/ps/products/126/ab126628/Images/ab126628-140630-anti-pdlim1-antibody-epr7187-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PDLIM1/CLP36 antibody [EPR7187] (ab126628)ab126628, at 1/250, staining PDLIM1/CLP36 in HeLa cells by Immunofluorescence.
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![Anti-PDLIM1/CLP36 antibody [EPR7187] (ab126628)](https://www.abcam.com/ps/products/126/ab126628/Images/ab126628-3-benefits-of-recombinant-antibodies.png)
Anti-PDLIM1/CLP36 antibody [EPR7187] (ab126628)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab126628 has been referenced in 1 publication.
- Hayashi G & Cortopassi G Lymphoblast Oxidative Stress Genes as Potential Biomarkers of Disease Severity and Drug Effect in Friedreich's Ataxia. PLoS One 11:e0153574 (2016). WB . PubMed: 27078885