Anti-PDP2 antibody [MS41A 3C3BC10] (ab133982)
![Western blot - Anti-PDP2 antibody [MS41A 3C3BC10] (ab133982)](https://www.abcam.com/ps/products/133/ab133982/Images/ab133982-148105-anti-pdp2-antibody-ms41a-3c3bc10-western-blot.jpg "Western blot - Anti-PDP2 antibody [MS41A 3C3BC10] (ab133982)")
Key features and details
- Mouse monoclonal [MS41A 3C3BC10] to PDP2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-PDP2 antibody [MS41A 3C3BC10]
See all PDP2 primary antibodies -
Description
Mouse monoclonal [MS41A 3C3BC10] to PDP2 -
Host species
Mouse -
Specificity
ab133982 does not react with Human Recombinant PDP1 or Human Recombinant PDK. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Full length protein. This information is considered to be commercially sensitive.
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Positive control
- Recombinant PDP2 protein, Human heart or liver homogenate, HeLa, HEK293, HepG2 and jurkat whole cell lysate.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: 99% HEPES buffered saline -
Concentration information loading... -
Purity
Ammonium Sulphate Precipitation -
Purification notes
Near homogeneity as judged by SDS-PAGE. ab133982 was produced in vitro using hybridomas grown in serum-free medium, and then concentrated by ammonium sulfate precipitation. -
Clonality
Monoclonal -
Clone number
MS41A 3C3BC10 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133982 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
Note: This antibody works optimally in 5% Milk for all blocking and antibody steps. |
| Notes |
|---|
|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa). Note: This antibody works optimally in 5% Milk for all blocking and antibody steps. |
Target
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Function
Catalyzes the dephosphorylation and concomitant reactivation of the alpha subunit of the E1 component of the pyruvate dehydrogenase complex. -
Sequence similarities
Belongs to the PP2C family. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
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Database links
- Entrez Gene: 57546 Human
- SwissProt: Q9P2J9 Human
- Unigene: 632214 Human
- Unigene: 654693 Human
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Alternative names
- [Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 2 antibody
- KIAA1348 antibody
- mitochondrial antibody
see all
Images
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Western blot - Anti-PDP2 antibody [MS41A 3C3BC10] (ab133982)All lanes : Anti-PDP2 antibody [MS41A 3C3BC10] (ab133982) at 1 µg/ml
Lane 1 : Molecular weight marker
Lane 2 : Recombinant human PDP2 protein (ab110358) at 0.01 µg
Lane 3 : Recombinant Human PDP1/PDP protein (His tag) (ab110357) at 0.01 µg
Lane 4 : Recombinant human PDK1 protein (ab110359) at 0.01 µg
Lane 5 : HHH (Human heart homogenate) at 20 µg
Lane 6 : HLH (Human liver homogenate) at 20 µg
Lane 7 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 8 : HEK293 (Human embryonic kidney 293 cell line) Whole Cell Lysate at 10 µg
Lane 9 : HepG2 (Human hepatocellular carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 10 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg
Secondary
All lanes : Goat polyclonal to Mouse IgG – H&L – Pre-Adsorbed (HRP) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 60 kDa
Observed band size: 60 kDa
Exposure time: 5 minutes
Predicted mature band size: 52 kDa
Observed band size: 52 kDa
Note: The antibody recognizes both the mature (52 kDa) and the immature (60 kDa) versions of PDP2 protein. The immunogen was mature PDP2 protein, lacking the first 66 amino acids which are used as a transit peptide for mitochondrial targeting. The antibody produces a band against the endogenous protein at the mature weight in tissue homogenates, but not in cancer cells where the protein may fail to mature.
Datasheets and documents
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Datasheet download
References (2)
ab133982 has been referenced in 2 publications.
- Ryan ZC et al. 1a,25-dihydroxyvitamin D3mitigates cancer cell mediated mitochondrial dysfunction in human skeletal muscle cells. Biochem Biophys Res Commun 496:746-752 (2018). WB . PubMed: 29366785
- Ryan ZC et al. 1a,25-Dihydroxyvitamin D3 Regulates Mitochondrial Oxygen Consumption and Dynamics in Human Skeletal Muscle Cells. J Biol Chem 291:1514-28 (2016). PubMed: 26601949
