Anti-PDX1 antibody (ab47267)

Thank you for contacting Abcam.

Currently we only sell this product in 100ul sizes, but if you are interested in placing a large bulk order (greater to 10 units) then we could look into a custom order.

If this is something that you would be interested in, please let me know and I will pass your information to our sales department.

Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody. I appreciate your patience, it has taken some time to obtain the testing protocol for you.

I would like to reassure you that this antibody is tested and covered by our 6 month guarantee for IHC-P and rat samples. In the event that a product is not functioning in the tested applications and species cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund.

I would like to investigate this particular case further for you, and also obtain more information for our quality monitoring records. In order to proceed with this, I have enclosed a technical questionnaire below. I would appreciate if you could complete this. It will help you put the information we require together very easily. Once I have this information, I hope I may be able to make some suggestions to improve the results.

I would appreciate if you could also provide an image which would help us to assess the results.

I have also copied below the testing IHC-P protocol from the originator which I hope will be useful. Please note that this would be a guideline only, and further individual optimization may often be required.

Thank you for your time and cooperation. We look forward to receiving the completed questionaire.

Order Details
Antibody code:

Lot number:

Purchase order number
or preferably Abcam order number:


General Information
Antibody storage conditions (temperature/reconstitution etc)


Description of the problem (high background, low signal, non-specific satining etc.)

Sample (Species/Tissue/Cell Type/Cell Line etc.)


Fixation of sample (Ethanol/Methanol/Acetone/Paraformaldehyde/Other/Duration etc.)


Antigen retrieval (Enzymatic method, Heat mediated technique etc.)


Permeabilization step


Blocking conditions (Buffer/time period, Blocking agent etc.)


Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)


Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)


Detection method


Positive and negative controls used (please specify)


Optimization attempts (problem solving)
How many times have you tried the IHC?



Have you run a No Primary control?
Yes No

Do you obtain the same results every time?
Yes No


What steps have you altered?


Additional Notes


We would appreciate if you are also able to provide and image which woudl help us to assess the results


IHC-P protocol:
Immunohistochemistry:

Day I



Dewax and rehydrate as normal.
Antigen Retrieval: Heat slides in 90 ml 10mM sodium citrate for 9 min. at 30% power in a 600 watt microwave. (Solution should reach 90-95O C but not boil.) Cool to room temp,. ˜30 min.
Wash in PBS, 3 x 2 min.
Quench endogenous peroxidase activity in 3%H2O2 for 20 min.
Wash in PBS, 3 x 2 min.
Drop on 5% normal goat serum to cover sample. Inc. 1h, RT. Use 50 ml/ml serum in PBS + 1% BSA.
Dilute a-pdx1 antibody 1:60,000 in PBS + 1%BSA. Shake off blocking solution and drop on antibody to cover sample. Inc. overnight, 4O C in humidity chamber.



Day II



Shake off the antibody solution and wash slides in PBS, 3 x 2 min.
Drop on biotinylated goat a-rabbit antibody (Diluted 1:1000 in PBS + 1%BSA) to cover samples. Incubate 1 h, RT.
After 2O Ab has been on samples for 30 min., make up ABC solution: Add 20 ml/ml reagent A + 20 ml/ml reagent B in PBS, and mix well (it has to sit for 30 min.).
After 1 h incubation, wash slides in PBS, 3 x 2 min.
Drop on ABC mixture to cover each sample, and incubate for 30 min. RT.
Wash slides in PBS, 3 x 2 min.
Drop on DAB mixture to cover samples, 1-5 min. at room temp. (DAB = 1 drop each of A, B, and C/ml dH2O; from Zymed Labs). Watch reaction on dissecting microscope until desired brown is attained.
Wash in dH2O, 3 x 1 min.
Counterstain with hematoxylin (or methyl green) 30 sec. – 1 min.
Wash in dH2O for 30 seconds (or in isopropanol if methyl green used).
Dehydrate as usual.