Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
RecombinantRabMAb

Recombinant Anti-PDX1 antibody [EPR3358(2)] (ab134150)

Reviews (1) Submit a question References (10)
Western blot - Anti-PDX1 antibody [EPR3358(2)] (ab134150)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody [EPR3358(2)] (ab134150)
  • Immunocytochemistry/ Immunofluorescence - Anti-PDX1 antibody [EPR3358(2)] (ab134150)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody [EPR3358(2)] (ab134150)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody [EPR3358(2)] (ab134150)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody [EPR3358(2)] (ab134150)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody [EPR3358(2)] (ab134150)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody [EPR3358(2)] (ab134150)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody [EPR3358(2)] (ab134150)
  • OI-RD Scanning - Anti-PDX1 antibody [EPR3358(2)] (ab134150)
  • Anti-PDX1 antibody [EPR3358(2)] (ab134150)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3358(2)] to PDX1
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Carrier Free

Overview

  • Product name

    Anti-PDX1 antibody [EPR3358(2)]
    See all PDX1 primary antibodies

  • Description

    Rabbit monoclonal [EPR3358(2)] to PDX1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide within Human PDX1 (C terminal). The exact sequence is proprietary.
    Database link: P52945

  • Positive control

    • CACO-2 and Beta-TC-6 cell lysates, Human pancreas & duodenum tissue

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab134150 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Predicted molecular weight: 30 kDa.
IHC-P (1)
1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/350.
ICC/IF
Use at an assay dependent concentration.
Notes
WB
1/1000. Predicted molecular weight: 30 kDa.
IHC-P
1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/350.
ICC/IF
Use at an assay dependent concentration.

Target

  • Function

    Activates insulin, somatostatin, glucokinase, islet amyloid polypeptide and glucose transporter type 2 gene transcription. Particularly involved in glucose-dependent regulation of insulin gene transcription. Binds preferentially the DNA motif 5'-[CT]TAAT[TG]-3'. During development, specifies the early pancreatic epithelium, permitting its proliferation, branching and subsequent differentiation. At adult stage, required for maintaining the hormone-producing phenotype of the beta-cell.

  • Tissue specificity

    Duodenum and pancreas (Langerhans islet beta cells and small subsets of endocrine non-beta-cells, at low levels in acinar cells).

  • Involvement in disease

    Defects in PDX1 are a cause of pancreatic agenesis (PAC) [MIM:260370]. This autosomal recessive disorder is characterized by absence or hypoplasia of pancreas, leading to early-onset insulin-dependent diabetes mellitus. This was found in a frameshift mutation that produces a truncated protein and results in a second initiation that produces a second protein that act as a dominant negative mutant.
    Defects in PDX1 are a cause of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853]; also known as diabetes mellitus type 2. NIDDM is characterized by an autosomal dominant mode of inheritance, onset during adulthood and insulin resistance.
    Defects in PDX1 are the cause of maturity-onset diabetes of the young type 4 (MODY4) [MIM:606392]; also symbolized MODY-4. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.

  • Sequence similarities

    Belongs to the Antp homeobox family. IPF1/XlHbox-8 subfamily.
    Contains 1 homeobox DNA-binding domain.

  • Domain

    The Antp-type hexapeptide mediates heterodimerization with PBX on a regulatory element of the somatostatin promoter.
    The homeodomain, which contains the nuclear localization signal, not only mediates DNA-binding, but also acts as a protein-protein interaction domain for TCF3(E47), NEUROD1 and HMG-I(Y).

  • Post-translational
    modifications

    Phosphorylated by the SAPK2 pathway at high intracellular glucose concentration.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • Glucose sensitive factor antibody
      • Glucose-sensitive factor antibody
      • GSF antibody
      • IDX 1 antibody
      • IDX-1 antibody
      • IDX1 antibody
      • Insulin promoter factor 1 antibody
      • Insulin promoter factor 1 homeodomain transcription factor antibody
      • Insulin upstream factor 1 antibody
      • IPF 1 antibody
      • IPF-1 antibody
      • IPF1 antibody
      • Islet/duodenum homeobox 1 antibody
      • Islet/duodenum homeobox-1 antibody
      • IUF 1 antibody
      • IUF-1 antibody
      • IUF1 antibody
      • MODY4 antibody
      • Pancreas/duodenum homeobox 1 antibody
      • Pancreas/duodenum homeobox protein 1 antibody
      • pancreatic and duodenal homeobox P antibody
      • PDX 1 antibody
      • PDX-1 antibody
      • PDX1 antibody
      • PDX1_HUMAN antibody
      • Somatostatin transactivating factor 1 antibody
      • Somatostatin-transactivating factor 1 antibody
      • STF 1 antibody
      • STF-1 antibody
      • STF1 antibody
      see all

    Images

    • Western blot - Anti-PDX1 antibody [EPR3358(2)] (ab134150)
      Western blot - Anti-PDX1 antibody [EPR3358(2)] (ab134150)
      All lanes : Anti-PDX1 antibody [EPR3358(2)] (ab134150) at 1/1000 dilution

      Lane 1 : Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate
      Lane 2 : BxPC-3 (Human pancreas adenocarcinoma epithelial cell) whole cell lysate
      Lane 3 : Beta-TC-6 (Mouse pancreas insulinoma beta cell) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Predicted band size: 30 kDa
      Observed band size: 40 kDa why is the actual band size different from the predicted?



      The expression profile observed in BxPC-3 is consistent with the literature (PMID: 12947327). Negative control:   BxPC-3 (PMID: 12947327)

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody [EPR3358(2)] (ab134150)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody [EPR3358(2)] (ab134150)

      ab134150 staining PDX1 in Human pancreas tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/500). An undiluted HRP-conjugated anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin. PBS was used in the negative control rather than the Primary antibody.

    • Immunocytochemistry/ Immunofluorescence - Anti-PDX1 antibody [EPR3358(2)] (ab134150)
      Immunocytochemistry/ Immunofluorescence - Anti-PDX1 antibody [EPR3358(2)] (ab134150)

      ab134150 staining PDX1 in the BXPC-3 cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde. Samples were incubated with primary antibody (1/150). An Alexa Fluor®555-conjugated Goat anti-rabbit IgG (1/500) was used as the secondary antibody. Nuclei were counterstained with DAPI.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody [EPR3358(2)] (ab134150)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody [EPR3358(2)] (ab134150)

      Immunohistochemical analysis of paraffin embedded Human pancreas tissue labelled with ab134150, unpurified, at 1/1000 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody [EPR3358(2)] (ab134150)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody [EPR3358(2)] (ab134150)

      Immunohistochemical analysis of paraffin embedded Human duodenum tissue labelled with ab134150, unpurified, at 1/1000 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody [EPR3358(2)] (ab134150)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody [EPR3358(2)] (ab134150)This image is courtesy of an anonymous Abreview

      ab134150, unpurified, staining PDX1 in Human pancreas tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% serum for 1 hour at 24°C; antigen retrieval was by heat mediation in 10mM sodium citrate, pH 6.0. Samples were incubated with primary antibody (1/1000 in 2% BSA) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated Donkey anti-rabbit IgG polyclonal (1/400) was used as the secondary antibody.

      See Abreview

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody [EPR3358(2)] (ab134150)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody [EPR3358(2)] (ab134150)

      Immunohistochemical analysis of paraffin embedded Human Colonic adenocarcinoma tissue using ab134150, unpurified, showing +ve staining.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody [EPR3358(2)] (ab134150)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody [EPR3358(2)] (ab134150)

      Immunohistochemical analysis of paraffin embedded Human Heart muscles tissue using ab134150, unpurified, showing -ve staining.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody [EPR3358(2)] (ab134150)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody [EPR3358(2)] (ab134150)

      Immunohistochemical analysis of paraffin embedded normal Human Normal brain tissue using ab134150, unpurified, showing -ve staining.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • OI-RD Scanning - Anti-PDX1 antibody [EPR3358(2)] (ab134150)
      OI-RD Scanning - Anti-PDX1 antibody [EPR3358(2)] (ab134150)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-PDX1 antibody [EPR3358(2)] (ab134150)
      Anti-PDX1 antibody [EPR3358(2)] (ab134150)

    References (10)

    Publishing research using ab134150? Please let us know so that we can cite the reference in this datasheet.

    ab134150 has been referenced in 10 publications.

    • Liang Z  et al. CircRNA circ-OGDH (hsa_circ_0003340) Acts as a ceRNA to Regulate Glutamine Metabolism and Esophageal Squamous Cell Carcinoma Progression by the miR-615-5p/PDX1 Axis. Cancer Manag Res 13:3041-3053 (2021). PubMed: 33854374
    • Ishikawa A  et al. Annexin A10 is involved in the induction of pancreatic duodenal homeobox-1 in gastric cancer tissue, cells and organoids. Oncol Rep 43:581-590 (2020). PubMed: 31789399
    • Hackeng WM  et al. Assessment of ARX expression, a novel biomarker for metastatic risk in pancreatic neuroendocrine tumors, in endoscopic ultrasound fine-needle aspiration. Diagn Cytopathol 48:308-315 (2020). PubMed: 31846235
    • Hackeng WM  et al. Alternative Lengthening of Telomeres and Differential Expression of Endocrine Transcription Factors Distinguish Metastatic and Non-metastatic Insulinomas. Endocr Pathol 31:108-118 (2020). PubMed: 32103422
    • Suthamwong P  et al. Administration of mulberry leaves maintains pancreatic ß-cell mass in obese/type 2 diabetes mellitus mouse model. BMC Complement Med Ther 20:136 (2020). PubMed: 32375753
    • Cejas P  et al. Enhancer signatures stratify and predict outcomes of non-functional pancreatic neuroendocrine tumors. Nat Med 25:1260-1265 (2019). PubMed: 31263286
    • Dirice E  et al. Human duct cells contribute to ß cell compensation in insulin resistance. JCI Insight 4:N/A (2019). PubMed: 30996131
    • Li W  et al. The Prognosis Value of PIWIL1 and PIWIL2 Expression in Pancreatic Cancer. J Clin Med 8:N/A (2019). PubMed: 31443431
    • Ma Y & Fei X SIRT5 regulates pancreatic ß-cell proliferation and insulin secretion in type 2 diabetes. Exp Ther Med 16:1417-1425 (2018). PubMed: 30116390
    • Roy S  et al. Rare somatic cells from human breast tissue exhibit extensive lineage plasticity. Proc Natl Acad Sci U S A 110:4598-603 (2013). IHC-P, ICC/IF ; Human . PubMed: 23487770

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PDX1 antibody [EPR3358(2)]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mm sodium citrate PH 6.0
    Sample
    Human Tissue sections (pancreas)
    Specification
    pancreas
    Permeabilization
    No
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Oct 01 2013

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com