Recombinant PE Anti-Caveolin-1 antibody [EPR15554] - N-terminal (ab212007)
![Flow Cytometry (Intracellular) - PE Anti-Caveolin-1 antibody [EPR15554] - N-terminal (ab212007)](https://www.abcam.com/ps/products/212/ab212007/Images/ab212007-264934-anti-caveolin-1-antibody-epr15554-n-terminal-phycoerythrin-flow-cytometry.jpg "Flow Cytometry (Intracellular) - PE Anti-Caveolin-1 antibody [EPR15554] - N-terminal (ab212007)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PE Rabbit monoclonal [EPR15554] to Caveolin-1 - N-terminal
- Suitable for: Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
Related conjugates and formulations
Overview
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Product name
PE Anti-Caveolin-1 antibody [EPR15554] - N-terminal
See all Caveolin-1 primary antibodies -
Description
PE Rabbit monoclonal [EPR15554] to Caveolin-1 - N-terminal -
Host species
Rabbit -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
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Immunogen
Recombinant fragment within Human Caveolin-1 aa 1-100 (N terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q03135 -
Positive control
- Flow Cyt (intra): A549 cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark. -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15554 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab212007 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/500.
The cellular localisation of this product has been verified in ICC/IF. |
| Notes |
|---|
|
Flow Cyt (Intra)
1/500. The cellular localisation of this product has been verified in ICC/IF. |
Target
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Function
May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity (By similarity). Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway. -
Tissue specificity
Expressed in muscle and lung, less so in liver, brain and kidney. -
Involvement in disease
Defects in CAV1 are the cause of congenital generalized lipodystrophy type 3 (CGL3) [MIM:612526]; also called Berardinelli-Seip congenital lipodystrophy type 3 (BSCL3). Congenital generalized lipodystrophies are autosomal recessive disorders characterized by a near absence of adipose tissue, extreme insulin resistance, hypertriglyceridemia, hepatic steatosis and early onset of diabetes. -
Sequence similarities
Belongs to the caveolin family. -
Post-translational
modificationsThe initiator methionine for isoform Beta is removed during or just after translation. The new N-terminal amino acid is then N-acetylated. -
Cellular localization
Golgi apparatus membrane. Cell membrane. Membrane > caveola. Membrane raft. Colocalized with DPP4 in membrane rafts. Potential hairpin-like structure in the membrane. Membrane protein of caveolae. - Information by UniProt
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Database links
- Entrez Gene: 857 Human
- Entrez Gene: 12389 Mouse
- Omim: 601047 Human
- SwissProt: Q03135 Human
- SwissProt: P49817 Mouse
- Unigene: 74034 Human
- Unigene: 28278 Mouse
- Unigene: 470537 Mouse
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Alternative names
- BSCL3 antibody
- CAV antibody
- CAV1 antibody
see all
Images
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Flow Cytometry (Intracellular) - PE Anti-Caveolin-1 antibody [EPR15554] - N-terminal (ab212007)Overlay histogram showing A549 cells stained with ab212007 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 90% methanol at -20°C for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab212007, 1/500 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter. -
![PE Anti-Caveolin-1 antibody [EPR15554] - N-terminal (ab212007)](https://www.abcam.com/ps/products/212/ab212007/Images/ab212007-3-benefits-of-recombinant-antibodies.png)
PE Anti-Caveolin-1 antibody [EPR15554] - N-terminal (ab212007)
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab212007 has not yet been referenced specifically in any publications.