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PE Anti-CD13 antibody [WM15], prediluted (ab69775)

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Key features and details

  • PE Mouse monoclonal [WM15] to CD13, prediluted
  • Suitable for: Flow Cyt
  • Reacts with: Human
  • Conjugation: PE. Ex: 488nm, Em: 575nm
  • Isotype: IgG1

Overview

  • Product name

    PE Anti-CD13 antibody [WM15], prediluted
    See all CD13 primary antibodies

  • Description

    PE Mouse monoclonal [WM15] to CD13, prediluted

  • Host species

    Mouse

  • Conjugation

    PE. Ex: 488nm, Em: 575nm

  • Tested applications

    Suitable for: Flow Cytmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Non human primates

  • Immunogen

    Tissue, cells or virus corresponding to Human CD13. Human AML cells

  • General notes

    The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab69775 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
Use at an assay dependent concentration.

or 100 ul of whole blood. ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Notes
Flow Cyt
Use at an assay dependent concentration.

or 100 ul of whole blood. ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Broad specificity aminopeptidase. Plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. May play a critical role in the pathogenesis of cholesterol gallstone disease. May be involved in the metabolism of regulatory peptides of diverse cell types including small intestinal and tubular epithelial cells, macrophages, granulocytes and synaptic membranes from the CNS. Found to cleave antigen peptides bound to major histocompatibility complex class II molecules of presenting cells and to degrade neurotransmitters at synaptic junctions. Is also implicated as a regulator of IL-8 bioavailability in the endometrium, and therefore may contribute to the regulation of angiogenesis. Is used as a marker for acute myeloid leukemia and plays a role in tumor invasion. In case of human coronavirus 229E (HCoV-229E) infection, serves as receptor for HCoV-229E spike glycoprotein. Mediates as well human cytomegalovirus (HCMV) infection.

  • Tissue specificity

    Expressed in epithelial cells of the kidney, intestine, and respiratory tract; granulocytes, monocytes, fibroblasts, endothelial cells, cerebral pericytes at the blood-brain barrier, synaptic membranes of cells in the CNS. Also expressed in endometrial stromal cells, but not in the endometrial glandular cells. Found in the vasculature of tissues that undergo angiogenesis and in malignant gliomas and lymph node metastases from multiple tumor types but not in blood vessels of normal tissues. A soluble form has been found in plasma. It is found to be elevated in plasma and effusions of cancer patients.

  • Sequence similarities

    Belongs to the peptidase M1 family.

  • Domain

    Amino acids 260-353 are essential to mediate susceptibility to infection with HCoV-229E (in porcine/human chimeric studies) and more specifically amino acids 288-295 (mutagenesis studies).

  • Post-translational
    modifications

    Sulfated.
    N- and O-glycosylated.
    May undergo proteolysis and give rise to a soluble form.

  • Cellular localization

    Cell membrane. Cytoplasm > cytosol. A soluble form has also been detected.
  • Information by UniProt

  • Database links

    • Alternative names

      • Alanyl (membrane) aminopeptidase antibody
      • Alanyl aminopeptidase antibody
      • Aminopeptidase M antibody
      • Aminopeptidase N antibody
      • AMPN_HUMAN antibody
      • ANPEP antibody
      • AP M antibody
      • AP N antibody
      • AP-M antibody
      • AP-N antibody
      • APN antibody
      • CD 13 antibody
      • CD13 antibody
      • CD13 antigen antibody
      • gp150 antibody
      • hAPN antibody
      • LAP 1 antibody
      • LAP1 antibody
      • Microsomal aminopeptidase antibody
      • Myeloid plasma membrane glycoprotein CD13 antibody
      • p150 antibody
      • PEPN antibody
      see all

    References (5)

    Publishing research using ab69775? Please let us know so that we can cite the reference in this datasheet.

    ab69775 has been referenced in 5 publications.

    • Kessler T  et al. Aminopeptidase N (CD13): Expression, Prognostic Impact, and Use as Therapeutic Target for Tissue Factor Induced Tumor Vascular Infarction in Soft Tissue Sarcoma. Transl Oncol 11:1271-1282 (2018). PubMed: 30125801
    • Schmidt LH  et al. Potential therapeutic impact of CD13 expression in non-small cell lung cancer. PLoS One 12:e0177146 (2017). Flow Cyt ; Human . PubMed: 28604784
    • Favaloro EJ  et al. CD13 (GP150; aminopeptidase-N): predominant functional activity in blood is localized to plasma and is not cell-surface associated. Exp Hematol 21:1695-701 (1993). PubMed: 7902291
    • Bradstock KF  et al. Human myeloid differentiation antigens identified by monoclonal antibodies: expression on leukemic cells. Pathology 17:392-9 (1985). PubMed: 3865147
    • Bradstock KF  et al. Myeloid progenitor surface antigen identified by monoclonal antibody. Br J Haematol 61:11-20 (1985). PubMed: 4052321

    Customer reviews and Q&As

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