PE Anti-CD3 antibody [KT3] (ab22268)
Key features and details
- PE Rat monoclonal [KT3] to CD3
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG2a
Related conjugates and formulations
Overview
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Product name
PE Anti-CD3 antibody [KT3]
See all CD3 primary antibodies -
Description
PE Rat monoclonal [KT3] to CD3 -
Host species
Rat -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Specificity
This antibody recognises the mouse CD3 antigen which is expressed by mature T cells. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Full length native protein (purified) corresponding to Mouse CD3.
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Positive control
- Flow Cyt: Mouse spleen, Canine blood leucocytes, Human PBMC
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General notes
Purified IgG conjugated to R. Phycoerythrin (RPE).
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 1% BSA, 5% Sucrose -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
KT3 -
Myeloma
NS0 -
Isotype
IgG2a -
Research areas
Associated products
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Alternative Versions
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab22268 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt | (7) |
Use 10µl for 106 cells.
ab134674 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Notes |
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Flow Cyt
Use 10µl for 106 cells. ab134674 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Images
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ab22268 staining CD3 in mouse spleen cells by Flow Cytometry analysis.
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ab22268 used in Flow Cytometry.
Canine blood leucocytes were harvested in PBS, 2% BSA, 0.01% sodium azide after red blood cell lysis, then fixed with paraformaldehyde. ab22268 used at a 1/100 dilution for 1 hour at 4°C.
The gating strategy used was for lymphocytes. -
Flow cytometry analysis of human peripheral blood mononuclear cells (PBMC) labelling CD3 with ab22268 at a dilution of (1/100 in PBS + 0.5% BSA) for 1 hour at 4°C. Cell separation was achieved by FICOLL medium with PBS + 0.5% BSA.
Gating Strategy: Lymphocytes
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab22268 has been referenced in 2 publications.
- Liu C et al. A nanovaccine for antigen self-presentation and immunosuppression reversal as a personalized cancer immunotherapy strategy. Nat Nanotechnol 17:531-540 (2022). PubMed: 35410368
- DeClue AE et al. Identification of immunologic and clinical characteristics that predict inflammatory response to C. Novyi-NT bacteriolytic immunotherapy. BMC Vet Res 14:119 (2018). Dog . PubMed: 29606140