PE Anti-CD34 antibody [4H11(APG)], prediluted (ab18228)
![Flow Cytometry - PE Anti-CD34 antibody [4H11(APG)], prediluted (ab18228)](https://www.abcam.com/ps/products/18/ab18228/Images/ab18228-27527-anti-cd34-antibody-4h11apg-prediluted-phycoerythrin-flow-cytometry.jpg "Flow Cytometry - PE Anti-CD34 antibody [4H11(APG)], prediluted (ab18228)")
Key features and details
- PE Mouse monoclonal [4H11(APG)] to CD34, prediluted
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
PE Anti-CD34 antibody [4H11(APG)], prediluted
See all CD34 primary antibodies -
Description
PE Mouse monoclonal [4H11(APG)] to CD34, prediluted -
Host species
Mouse -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Specificity
The antibody reacts with Class III epitope on CD34 antibody. It completely blocks binding of Class II antibody QBEnd10 and Class III antibodies BIRMA K3 and 8G12 on KG1a cell line -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human CD34. Permanent human cell line derived from peripheral leucocytes of a patient suffering from chronic myeloid leukaemia
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Positive control
- CML cell line MOLM-7.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: PBS, BSA -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Conjugate purified by size-exclusion chromatography. -
Clonality
Monoclonal -
Clone number
4H11(APG) -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab18228 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | (1) |
Use 20µl for 106 cells.
A cell suspension volume of 100 µl is recommended.
ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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| Notes |
|---|
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Flow Cyt
Use 20µl for 106 cells. A cell suspension volume of 100 µl is recommended.
ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|
Target
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Function
Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins. -
Tissue specificity
Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues. -
Sequence similarities
Belongs to the CD34 family. -
Developmental stage
On early hematopoietic progenitor cells. -
Post-translational
modificationsHighly glycosylated.
Phosphorylated on serine residues by PKC. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 947 Human
- Omim: 142230 Human
- SwissProt: P28906 Human
- Unigene: 374990 Human
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Alternative names
- CD34 antibody
- CD34 antigen antibody
- CD34 molecule antibody
see all
Images
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Flow Cytometry - PE Anti-CD34 antibody [4H11(APG)], prediluted (ab18228)
ab18228 staining CD34 in KG-1a cell line by Flow Cytometery. Blue peak repersents negative control while red peak show signal with ab18228.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab18228 has been referenced in 2 publications.
- Sun KT et al. Reciprocal regulation of ?-globin expression by exo-miRNAs: Relevance to ?-globin silencing in ß-thalassemia major. Sci Rep 7:202 (2017). PubMed: 28303002
- Castro FV et al. 5T4 oncofetal antigen is expressed in high risk of relapse childhood pre-B acute lymphoblastic leukemia and is associated with a more invasive and chemotactic phenotype. Leukemia 26:1487-98 (2012). Flow Cyt ; Human . PubMed: 22266911