PE Anti-CD34 antibody [MEC 14.7] (ab23830)
Key features and details
- PE Rat monoclonal [MEC 14.7] to CD34
- Suitable for: IHC-P, IP, Flow Cyt
- Reacts with: Mouse
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG2a
Related conjugates and formulations
Overview
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Product name
PE Anti-CD34 antibody [MEC 14.7]
See all CD34 primary antibodies -
Description
PE Rat monoclonal [MEC 14.7] to CD34 -
Host species
Rat -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Specificity
Recognises the murine CD34 cell surface glycoprotein. Murine CD34 is expressed by haematopoietic progenitor cells in the bone marrow, and also by endothelial cells. -
Tested applications
Suitable for: IHC-P, IP, Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Cells transfected with recombinant full length protein (Mouse).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.40
Preservative: 0.09% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
MEC 14.7 -
Isotype
IgG2a -
Research areas
Associated products
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Alternative Versions
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab23830 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/20 - 1/200.
|
|
| IP |
Use a concentration of 2 - 10 µg/ml.
|
|
| Flow Cyt | (1) |
1/25 - 1/50.
Use 10μl of the suggested working dilution to label 106 cells in 100μl |
| Notes |
|---|
|
IHC-P
1/20 - 1/200. |
|
IP
Use a concentration of 2 - 10 µg/ml. |
|
Flow Cyt
1/25 - 1/50. Use 10μl of the suggested working dilution to label 106 cells in 100μl |
Target
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Function
Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins. -
Tissue specificity
Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues. -
Sequence similarities
Belongs to the CD34 family. -
Developmental stage
On early hematopoietic progenitor cells. -
Post-translational
modificationsHighly glycosylated.
Phosphorylated on serine residues by PKC. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 12490 Mouse
- SwissProt: Q64314 Mouse
- Unigene: 29798 Mouse
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Alternative names
- CD34 antibody
- CD34 antigen antibody
- CD34 molecule antibody
see all
Datasheets and documents
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Datasheet download
References (18)
ab23830 has been referenced in 18 publications.
- Luo H et al. Manufacturing and banking canine adipose-derived mesenchymal stem cells for veterinary clinical application. BMC Vet Res 17:96 (2021). PubMed: 33648493
- Wei W et al. Bone marrow mesenchymal stem cell exosomes suppress phosphate-induced aortic calcification via SIRT6-HMGB1 deacetylation. Stem Cell Res Ther 12:235 (2021). PubMed: 33849640
- Li D et al. Isolation and identification of exosomes from feline plasma, urine and adipose-derived mesenchymal stem cells. BMC Vet Res 17:272 (2021). PubMed: 34384449
- Cooke ME et al. 3D Printed Polyurethane Scaffolds for the Repair of Bone Defects. Front Bioeng Biotechnol 8:557215 (2020). PubMed: 33195122
- Ramirez-Garcia-Luna JL et al. Defective bone repair in diclofenac treated C57Bl6 mice with and without lipopolysaccharide induced systemic inflammation. J Cell Physiol 234:3078-3087 (2019). PubMed: 30191966
