Recombinant PE Anti-CDCA5 antibody [EPR16331] - C-terminal (ab225377)
![Flow Cytometry (Intracellular) - PE Anti-CDCA5 antibody [EPR16331] - C-terminal (ab225377)](https://www.abcam.com/ps/products/225/ab225377/Images/ab225377-326818-ab225377FLOW1.jpg "Flow Cytometry (Intracellular) - PE Anti-CDCA5 antibody [EPR16331] - C-terminal (ab225377)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PE Rabbit monoclonal [EPR16331] to CDCA5 - C-terminal
- Suitable for: Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
Related conjugates and formulations
Overview
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Product name
PE Anti-CDCA5 antibody [EPR16331] - C-terminal
See all CDCA5 primary antibodies -
Description
PE Rabbit monoclonal [EPR16331] to CDCA5 - C-terminal -
Host species
Rabbit -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow Cyt (intra): MCF7 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16331 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab225377 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/5000.
The cellular localisation of this product has been verified in ICC/IF. |
| Notes |
|---|
|
Flow Cyt (Intra)
1/5000. The cellular localisation of this product has been verified in ICC/IF. |
Target
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Function
Regulator of sister chromatid cohesion in mitosis stabilizing cohesin complex association with chromatin. May antagonize the action of WAPAL which stimulates cohesin dissociation from chromatin. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair. Required for efficient DNA double-stranded break repair. -
Sequence similarities
Belongs to the sororin family. -
Domain
The KEN box is required for the association with the APC/C complex. -
Post-translational
modificationsPhosphorylated. Phosphorylation, as cells enter mitosis, disrupts the interaction with PDS5A and relieves the inhibition of WAPAL by CDCA5.
Ubiquitinated by the APC/C complex in G1, leading to its degradation. -
Cellular localization
Nucleus. Chromosome. Cytoplasm. Associates with nuclear chromatin from S phase until metaphase and is released in the cytoplasm upon nuclear envelope breakdown. - Information by UniProt
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Database links
- Entrez Gene: 113130 Human
- Omim: 609374 Human
- SwissProt: Q96FF9 Human
- Unigene: 434886 Human
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Alternative names
- CDCA5 antibody
- CDCA5_HUMAN antibody
- Cell division cycle associated protein 5 antibody
see all
Images
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Flow Cytometry (Intracellular) - PE Anti-CDCA5 antibody [EPR16331] - C-terminal (ab225377)
Overlay histogram showing MCF7 cells stained with ab225377 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab225377, 1/5000 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.
This antibody gave a positive signal in MCF7 cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.
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![PE Anti-CDCA5 antibody [EPR16331] - C-terminal (ab225377)](https://www.abcam.com/ps/products/225/ab225377/Images/ab225377-3-benefits-of-recombinant-antibodies.png)
PE Anti-CDCA5 antibody [EPR16331] - C-terminal (ab225377)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab225377 has not yet been referenced specifically in any publications.