Recombinant PE Anti-Cytokeratin 19 antibody [EP1580Y] (ab224981)
![Flow Cytometry (Intracellular) - PE Anti-Cytokeratin 19 antibody [EP1580Y] (ab224981)](https://www.abcam.com/ps/products/224/ab224981/Images/ab224981-294882-anti-cytokeratin-19-antibody-ep1580y-phycoerythrin-flow-cytometry.jpg "Flow Cytometry (Intracellular) - PE Anti-Cytokeratin 19 antibody [EP1580Y] (ab224981)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PE Rabbit monoclonal [EP1580Y] to Cytokeratin 19
- Suitable for: Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
Related conjugates and formulations
Overview
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Product name
PE Anti-Cytokeratin 19 antibody [EP1580Y]
See all Cytokeratin 19 primary antibodies -
Description
PE Rabbit monoclonal [EP1580Y] to Cytokeratin 19 -
Host species
Rabbit -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow Cyt (intra): HeLa cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark. -
Dissociation constant (KD)
KD = 3.70 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1580Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Alexa Fluor® 488 Anti-Cytokeratin 19 antibody [EP1580Y] (ab192643)
- Alexa Fluor® 647 Anti-Cytokeratin 19 antibody [EP1580Y] (ab192980)
- HRP Anti-Cytokeratin 19 antibody [EP1580Y] (ab193600)
- Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (ab195872)
- Alexa Fluor® 594 Anti-Cytokeratin 19 antibody [EP1580Y] (ab203443)
- Alexa Fluor® 555 Anti-Cytokeratin 19 antibody [EP1580Y] (ab203444)
- Alexa Fluor® 568 Anti-Cytokeratin 19 antibody [EP1580Y] (ab203445)
- APC Anti-Cytokeratin 19 antibody [EP1580Y] (ab224980)
- Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker (ab52625)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab224981 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
Use at an assay dependent concentration.
The cellular localisation of this product has been verified in ICC/IF. |
| Notes |
|---|
|
Flow Cyt (Intra)
Use at an assay dependent concentration. The cellular localisation of this product has been verified in ICC/IF. |
Target
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Function
Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. -
Tissue specificity
Expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium (at protein level). Expressed in epidermal basal cells, in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord, and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin. -
Sequence similarities
Belongs to the intermediate filament family. -
Developmental stage
Present in hair follicles at all stages of development. -
Domain
This keratin differs from all other IF proteins in lacking the C-terminal tail domain. - Information by UniProt
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Database links
- Entrez Gene: 3880 Human
- Omim: 148020 Human
- SwissProt: P08727 Human
- Unigene: 654568 Human
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Alternative names
- 40 kDa keratin intermediate filament antibody
- CK 19 antibody
- CK-19 antibody
see all
Images
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Flow Cytometry (Intracellular) - PE Anti-Cytokeratin 19 antibody [EP1580Y] (ab224981)
Overlay histogram showing HeLa cells stained with ab224981 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab224981, 1/1000 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody.
Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.
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![PE Anti-Cytokeratin 19 antibody [EP1580Y] (ab224981)](https://www.abcam.com/ps/products/224/ab224981/Images/ab224981-3-benefits-of-recombinant-antibodies.png)
PE Anti-Cytokeratin 19 antibody [EP1580Y] (ab224981)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab224981 has not yet been referenced specifically in any publications.