Recombinant PE Anti-Eph receptor A2 antibody [EPR17660-120] (ab313756)
![Flow Cytometry - PE Anti-Eph receptor A2 antibody [EPR17660-120] (ab313756)](https://www.abcam.com/ps/products/313/ab313756/Images/ab313756-1-antiEphreceptorA2peepr17660120nih3t3flowcytometry.jpg "Flow Cytometry - PE Anti-Eph receptor A2 antibody [EPR17660-120] (ab313756)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PE Rabbit monoclonal [EPR17660-120] to Eph receptor A2
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PE. Ex: 488nm, Em: 575nm
Related conjugates and formulations
Overview
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Product name
PE Anti-Eph receptor A2 antibody [EPR17660-120]
See all Eph receptor A2 primary antibodies -
Description
PE Rabbit monoclonal [EPR17660-120] to Eph receptor A2 -
Host species
Rabbit -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow Cyt: NIH/3T3 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17660-120 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-Eph receptor A2 antibody [EPR17660-120] - BSA and Azide free (ab225579)
- Anti-Eph receptor A2 antibody [EPR17660-120] - BSA and Azide free (Detector) (ab242717)
- Alexa Fluor® 647 Anti-Eph receptor A2 antibody [EPR17660-120] (ab303471)
- Alexa Fluor® 488 Anti-Eph receptor A2 antibody [EPR17660-120] (ab305111)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab313756 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
1/500.
|
| Notes |
|---|
|
Flow Cyt
1/500. |
Target
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Function
Receptor for members of the ephrin-A family. Binds to ephrin-A1, -A3, -A4 and -A5. Plays an important role in angiogenesis and tumor neovascularization. The recruitement of VAV2, VAV3 and PI3-kinase p85 subunit by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly (By similarity). Induces apoptosis in a p53/TP53-independent, caspase-8-dependent manner. -
Tissue specificity
Expressed in brain and glioma tissue and glioma cell lines (at protein level). Expressed most highly in tissues that contain a high proportion of epithelial cells, e.g., skin, intestine, lung, and ovary. -
Involvement in disease
Genetic variations in EPHA2 are the cause of susceptibility to cataract cortical age-related type 2 (ARCC2) [MIM:613020]. A developmental punctate opacity common in the cortex and present in most lenses. The cataract is white or cerulean, increases in number with age, but rarely affects vision.
Defects in EPHA2 are the cause of cataract posterior polar type 1 (CTPP1) [MIM:116600]. A subcapsular opacity, usually disk-shaped, located at the back of the lens. It can have a marked effect on visual acuity. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
Contains 2 fibronectin type-III domains.
Contains 1 protein kinase domain.
Contains 1 SAM (sterile alpha motif) domain. -
Post-translational
modificationsActivated by EFNA1 via tyrosine phosphorylation. Phosphorylated residues Tyr-588 and Tyr-594 are required for binding VAV2 and VAV3 while phosphorylated residues Tyr-735 and Tyr-930 are required for binding PI3-kinase p85 subunit. These phosphorylated residues are critical for recruitment of VAV2 and VAV3 and PI3-kinase p85 subunit which transduce downstream signaling to activate RAC1 GTPase and endothelial cell migration. They also play a critical role in transducing EPHA2 signaling in vascular endothelial cells during tumor angiogenesis. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 13836 Mouse
- SwissProt: Q03145 Mouse
- Unigene: 2581 Mouse
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Alternative names
- ARCC2 antibody
- AW545284 antibody
- CTPA antibody
see all
Images
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Flow Cytometry - PE Anti-Eph receptor A2 antibody [EPR17660-120] (ab313756)
Flow cytometry overlay histogram showing NIH/3T3 positive cells (left panel) and negative B16-F10 (right panel) stained with ab313756 (red line). The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab313756) (1x 10⁶ in 100 µl at 1 µg/ml (1/500)) for 30 min at 4°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter.
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![PE Anti-Eph receptor A2 antibody [EPR17660-120] (ab313756)](https://www.abcam.com/ps/products/313/ab313756/Images/ab313756-515091-BenefitsofRecombinantantibodies.jpg)
PE Anti-Eph receptor A2 antibody [EPR17660-120] (ab313756)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab313756 has not yet been referenced specifically in any publications.