Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
RecombinantRabMAb

Recombinant PE Anti-ERK1 + ERK2 antibody [EPR17526] (ab212153)

Submit a review Submit a question References (1)
Flow Cytometry (Intracellular) - PE Anti-ERK1 + ERK2 antibody [EPR17526] (ab212153)
  • PE Anti-ERK1 + ERK2 antibody [EPR17526] (ab212153)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • PE Rabbit monoclonal [EPR17526] to ERK1 + ERK2
  • Suitable for: Flow Cyt (Intra)
  • Reacts with: Human
  • Conjugation: PE. Ex: 488nm, Em: 575nm

Conjugates logo Related conjugates and formulations

Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 594 Alexa Fluor® 647 Carrier Free HRP Unconjugated

Overview

  • Product name

    PE Anti-ERK1 + ERK2 antibody [EPR17526]
    See all ERK1 + ERK2 primary antibodies

  • Description

    PE Rabbit monoclonal [EPR17526] to ERK1 + ERK2

  • Host species

    Rabbit

  • Conjugation

    PE. Ex: 488nm, Em: 575nm

  • Tested applications

    Suitable for: Flow Cyt (Intra)more details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Recombinant fragment within Human ERK1 + ERK2 aa 150 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: P28482

  • Positive control

    • Flow Cyt (intra): A431 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab212153 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/2500.

The cellular localisation of this product has been verified in ICC/IF.
Notes
Flow Cyt (Intra)
1/2500.

The cellular localisation of this product has been verified in ICC/IF.

Target

  • Function

    Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2.
    Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.

  • Sequence similarities

    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.

  • Domain

    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

  • Post-translational
    modifications

    Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-187.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    see all

  • Form

    Mainly expressed in the cytoplasm and only localizes to the nucleus with treatment.

  • Alternative names

    • ERK 1 antibody
    • ERK 2 antibody
    • ERK-1 antibody
    • ERK-2 antibody
    • ERK1 antibody
    • erk1/2 antibody
    • ERK2 antibody
    • ERT1 antibody
    • ERT2 antibody
    • Extracellular signal regulated kinase 1 antibody
    • Extracellular signal-regulated kinase 1 antibody
    • Extracellular signal-regulated kinase 2 antibody
    • HS44KDAP antibody
    • HUMKER1A antibody
    • Insulin-stimulated MAP2 kinase antibody
    • MAP kinase 1 antibody
    • MAP kinase 2 antibody
    • MAP kinase 3 antibody
    • MAP kinase isoform p42 antibody
    • MAP kinase isoform p44 antibody
    • MAPK 1 antibody
    • MAPK 2 antibody
    • MAPK 3 antibody
    • Mapk1 antibody
    • MAPK2 antibody
    • MAPK3 antibody
    • Microtubule-associated protein 2 kinase antibody
    • Mitogen-activated protein kinase 1 antibody
    • Mitogen-activated protein kinase 2 antibody
    • Mitogen-activated protein kinase 3 antibody
    • MK01_HUMAN antibody
    • p38 antibody
    • p40 antibody
    • p41 antibody
    • p41mapk antibody
    • p42-MAPK antibody
    • P42MAPK antibody
    • p44-ERK1 antibody
    • p44-MAPK antibody
    • p44ERK1 antibody
    • p44MAPK antibody
    • PRKM 2 antibody
    • PRKM1 antibody
    • PRKM2 antibody
    • PRKM3 antibody
    • protein tyrosine kinase ERK2 antibody
    see all

Images

  • Flow Cytometry (Intracellular) - PE Anti-ERK1 + ERK2 antibody [EPR17526] (ab212153)
    Flow Cytometry (Intracellular) - PE Anti-ERK1 + ERK2 antibody [EPR17526] (ab212153)
    Overlay histogram showing A431 cells stained with ab212153 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 90% methanol at -20°C for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab212153, 1/2500 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.
  • PE Anti-ERK1 + ERK2 antibody [EPR17526] (ab212153)
    PE Anti-ERK1 + ERK2 antibody [EPR17526] (ab212153)

References (1)

Publishing research using ab212153? Please let us know so that we can cite the reference in this datasheet.

ab212153 has been referenced in 1 publication.

  • Hwang HJ  et al. The BTLA and PD-1 signaling pathways independently regulate the proliferation and cytotoxicity of human peripheral blood ?d T cells. Immun Inflamm Dis 9:274-287 (2021). PubMed: 33332777

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

There are currently no Customer reviews or Questions for ab212153.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com