Recombinant PE Anti-ERK1 + ERK2 antibody [EPR17526] (ab212153)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PE Rabbit monoclonal [EPR17526] to ERK1 + ERK2
- Suitable for: Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
Related conjugates and formulations
Overview
-
Product name
PE Anti-ERK1 + ERK2 antibody [EPR17526]
See all ERK1 + ERK2 primary antibodies -
Description
PE Rabbit monoclonal [EPR17526] to ERK1 + ERK2 -
Host species
Rabbit -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment within Human ERK1 + ERK2 aa 150 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P28482 -
Positive control
- Flow Cyt (intra): A431 cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark. -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17526 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
- Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)
- Alexa Fluor® 488 Anti-ERK1 + ERK2 antibody [EPR17526] (ab208564)
- Alexa Fluor® 647 Anti-ERK1 + ERK2 antibody [EPR17526] (ab208881)
- HRP Anti-ERK1 + ERK2 antibody [EPR17526] (ab209321)
- Alexa Fluor® 405 Anti-ERK1 + ERK2 antibody [EPR17526] (ab210044)
- Alexa Fluor® 594 Anti-ERK1 + ERK2 antibody [EPR17526] (ab210460)
- Alexa Fluor® 555 Anti-ERK1 + ERK2 antibody [EPR17526] (ab210659)
- Anti-ERK1 + ERK2 antibody [EPR17526] - BSA and Azide free (ab218017)
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab212153 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/2500.
The cellular localisation of this product has been verified in ICC/IF. |
Notes |
---|
Flow Cyt (Intra)
1/2500. The cellular localisation of this product has been verified in ICC/IF. |
Target
-
Function
Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2.
Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity. -
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain. -
Domain
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. -
Post-translational
modificationsDually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-187. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 5594 Human
- Entrez Gene: 5595 Human
- Entrez Gene: 26413 Mouse
- Entrez Gene: 26417 Mouse
- Entrez Gene: 116590 Rat
- Entrez Gene: 50689 Rat
- Omim: 176948 Human
- Omim: 601795 Human
see all -
Form
Mainly expressed in the cytoplasm and only localizes to the nucleus with treatment. -
Alternative names
- ERK 1 antibody
- ERK 2 antibody
- ERK-1 antibody
see all
Images
-
Overlay histogram showing A431 cells stained with ab212153 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 90% methanol at -20°C for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab212153, 1/2500 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.
Datasheets and documents
-
SDS download
-
Datasheet download
Certificate of Compliance
References (1)
ab212153 has been referenced in 1 publication.
- Hwang HJ et al. The BTLA and PD-1 signaling pathways independently regulate the proliferation and cytotoxicity of human peripheral blood ?d T cells. Immun Inflamm Dis 9:274-287 (2021). PubMed: 33332777