Recombinant PE Anti-Glycophorin C/GPC antibody [EPR4116] (ab306542)
![Flow Cytometry (Intracellular) - PE Anti-Glycophorin C/GPC antibody [EPR4116] (ab306542)](https://www.abcam.com/ps/products/306/ab306542/Images/ab306542-1-anti-glycophorin-c-gpc-antibody-epr4116-pe-k562-cells-flow-cytometry.jpg "Flow Cytometry (Intracellular) - PE Anti-Glycophorin C/GPC antibody [EPR4116] (ab306542)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PE Rabbit monoclonal [EPR4116] to Glycophorin C/GPC
- Suitable for: Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
Related conjugates and formulations
Overview
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Product name
PE Anti-Glycophorin C/GPC antibody [EPR4116]
See all Glycophorin C/GPC primary antibodies -
Description
PE Rabbit monoclonal [EPR4116] to Glycophorin C/GPC -
Host species
Rabbit -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow Cyt (Intra): K562 cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4116 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab306542 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/12500.
|
| Notes |
|---|
|
Flow Cyt (Intra)
1/12500. |
Target
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Function
This protein is a minor sialoglycoprotein in human erythrocyte membranes. The blood group Gerbich antigens and receptors for Plasmodium falciparum merozoites are most likely located within the extracellular domain. Glycophorin-C plays an important role in regulating the stability of red cells. -
Tissue specificity
Glycophorin-C is expressed in erythrocytes. Glycophorin-D is ubiquitous. -
Sequence similarities
Belongs to the glycophorin-C family. -
Cellular localization
Cell membrane. Linked to the membrane via band 4.1. - Information by UniProt
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Database links
- Entrez Gene: 2995 Human
- Omim: 110750 Human
- SwissProt: P04921 Human
- Unigene: 59138 Human
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Alternative names
- CD236 antibody
- CD236R antibody
- GE antibody
see all
Images
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Flow Cytometry (Intracellular) - PE Anti-Glycophorin C/GPC antibody [EPR4116] (ab306542)
Flow cytometry overlay histogram showing left K562 positive cells and right negative A549 cells stained with ab306542 (red line). The cells were fixed with 4 % formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10 % normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab306542) (1x 106 in 100μl at 0.04μg/ml (1/12500)) for 30 min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab306542 has not yet been referenced specifically in any publications.