Recombinant PE Anti-Glypican 3 antibody [SP86] (ab303587)
![Flow Cytometry - PE Anti-Glypican 3 antibody [SP86] (ab303587)](https://www.abcam.com/ps/products/303/ab303587/Images/ab303587-2-anti-glypican-3-antibody-sp86-pe-HAP1-flow-cytometry.jpg "Flow Cytometry - PE Anti-Glypican 3 antibody [SP86] (ab303587)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PE Rabbit monoclonal [SP86] to Glypican 3
- Suitable for: Flow Cyt
- Knockout validated
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
Related conjugates and formulations
Overview
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Product name
PE Anti-Glypican 3 antibody [SP86]
See all Glypican 3 primary antibodies -
Description
PE Rabbit monoclonal [SP86] to Glypican 3 -
Host species
Rabbit -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow Cyt: HAP1 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
SP86 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab303587 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
1/500.
|
| Notes |
|---|
|
Flow Cyt
1/500. |
Target
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Function
Cell surface proteoglycan that bears heparan sulfate. Inhibits the dipeptidyl peptidase activity of DPP4. May be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. May play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. May regulate growth and tumor predisposition. -
Tissue specificity
Highly expressed in lung, liver and kidney. -
Involvement in disease
Defects in GPC3 are the cause of Simpson-Golabi-Behmel syndrome type 1 (SGBS1) [MIM:312870]; also known as Simpson dysmorphia syndrome (SDYS). SGBS is a condition characterized by pre- and postnatal overgrowth (gigantism) with visceral and skeletal anomalies. -
Sequence similarities
Belongs to the glypican family. -
Cellular localization
Cell membrane and Secreted > extracellular space. - Information by UniProt
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Database links
- Entrez Gene: 2719 Human
- Omim: 300037 Human
- SwissProt: P51654 Human
- Unigene: 644108 Human
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Alternative names
- DGSX antibody
- Glypican proteoglycan 3 antibody
- Glypican-3 [Precursor] antibody
see all
Images
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Flow Cytometry - PE Anti-Glypican 3 antibody [SP86] (ab303587)
Flow cytometry overlay histogram showing wild-type HAP1 (green line) and GPC3 knockout HAP1 cells stained with AB303587 (red line). The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (AB303587) (1x106 in 100μl at 1μg/ml) for 30 min at 4°C.
Isotype control antibody Rabbit IgG (monoclonal) Phycoerythrin (ab209478) was used at the same concentration and conditions as the primary antibody (wild-type HAP1 - black line GPC3 knockout HAP1 - grey line). Unlabeled sample was also used as a control (this line is not shown for the purpose of simplicity).
Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561 nm) and 585/42 bandpass filter.
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![PE Anti-Glypican 3 antibody [SP86] (ab303587)](https://www.abcam.com/ps/products/303/ab303587/Images/ab303587-494244-MTOConjugatedRecombinantAntibodyBenefits.jpg)
PE Anti-Glypican 3 antibody [SP86] (ab303587) -
![PE Anti-Glypican 3 antibody [SP86] (AB303587)](https://www.abcam.com/ps/products/303/ab303587/Images/AB303587-1-Benefits-of-Recombinant-antibodies.jpg)
PE Anti-Glypican 3 antibody [SP86] (AB303587)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab303587 has not yet been referenced specifically in any publications.