Recombinant PE Anti-Integrin alpha V antibody [EPR16800] (ab211845)
![Flow Cytometry - PE Anti-Integrin alpha V antibody [EPR16800] (ab211845)](https://www.abcam.com/ps/products/211/ab211845/Images/ab211845-264933-anti-integrin-alpha-v-antibody-epr16800-phycoerythrin-flow-cytometry.jpg "Flow Cytometry - PE Anti-Integrin alpha V antibody [EPR16800] (ab211845)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PE Rabbit monoclonal [EPR16800] to Integrin alpha V
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
Related conjugates and formulations
Overview
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Product name
PE Anti-Integrin alpha V antibody [EPR16800]
See all Integrin alpha V primary antibodies -
Description
PE Rabbit monoclonal [EPR16800] to Integrin alpha V -
Host species
Rabbit -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Recombinant fragment within Human Integrin alpha V aa 1-250. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P06756 -
Positive control
- Flow Cyt: A549 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark. -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16800 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-Integrin alpha V antibody [EPR16800] (ab179475)
- Alexa Fluor® 647 Anti-Integrin alpha V antibody [EPR16800] (ab204684)
- HRP Anti-Integrin alpha V antibody [EPR16800] (ab205474)
- Alexa Fluor® 594 Anti-Integrin alpha V antibody [EPR16800] (ab207286)
- Anti-Integrin alpha V antibody [EPR16800] - Low endotoxin, Azide free (ab222222)
- Alexa Fluor® 488 Anti-Integrin alpha V antibody [EPR16800] (ab275350)
- Alexa Fluor® 555 Anti-Integrin alpha V antibody [EPR16800] (ab275351)
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab211845 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
1/500.
The cellular localisation of this product has been verified in ICC/IF. |
| Notes |
|---|
|
Flow Cyt
1/500. The cellular localisation of this product has been verified in ICC/IF. |
Target
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Function
The alpha-V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. -
Sequence similarities
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3685 Human
- Entrez Gene: 16410 Mouse
- Entrez Gene: 296456 Rat
- Omim: 193210 Human
- SwissProt: P06756 Human
- SwissProt: P43406 Mouse
- Unigene: 436873 Human
- Unigene: 227 Mouse
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Alternative names
- antigen identified by monoclonal antibody L230 antibody
- CD 51 antibody
- CD51 antibody
see all
Images
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Flow Cytometry - PE Anti-Integrin alpha V antibody [EPR16800] (ab211845)Overlay histogram showing A549 cells stained with ab211845 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 90% methanol at -20°C for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab211845, 1/500 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter. -
![PE Anti-Integrin alpha V antibody [EPR16800] (ab211845)](https://www.abcam.com/ps/products/211/ab211845/Images/ab211845-3-benefits-of-recombinant-antibodies.png)
PE Anti-Integrin alpha V antibody [EPR16800] (ab211845)
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab211845 has not yet been referenced specifically in any publications.