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    products/primary-antibodies/pe-neun-antibody-epr12763-neuronal-marker-ab306540.pdf

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Neuroscience Cell Type Marker Neuron marker Soma marker
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RecombinantRabMAb

Recombinant PE Anti-NeuN antibody [EPR12763] (Neuronal Marker) (ab306540)

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Flow Cytometry (Intracellular) - PE Anti-NeuN antibody [EPR12763] (Neuronal Marker) (ab306540)

    Key features and details

    • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
    • PE Rabbit monoclonal [EPR12763] to NeuN - Neuronal Marker
    • Suitable for: Flow Cyt (Intra)
    • Reacts with: Mouse
    • Conjugation: PE. Ex: 488nm, Em: 575nm

    Conjugates logo Related conjugates and formulations

    Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Biotin Carrier Free FITC Unconjugated

    Overview

    • Product name

      PE Anti-NeuN antibody [EPR12763] (Neuronal Marker)
      See all NeuN primary antibodies
    • Description

      PE Rabbit monoclonal [EPR12763] to NeuN - Neuronal Marker
    • Host species

      Rabbit
    • Conjugation

      PE. Ex: 488nm, Em: 575nm
    • Tested applications

      Suitable for: Flow Cyt (Intra)more details
    • Species reactivity

      Reacts with: Mouse
    • Immunogen

      Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

    • Positive control

      • Flow Cyt (Intra): Neuro 2a
    • General notes

      This product is a recombinant monoclonal antibody, which offers several advantages including:

      • - High batch-to-batch consistency and reproducibility
      • - Improved sensitivity and specificity
      • - Long-term security of supply
      • - Animal-free production
      For more information see here.

      Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Avoid freeze / thaw cycle. Store In the Dark.
    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituents: 98% PBS, 1% BSA
    • Concentration information loading...
    • Purity

      Protein A purified
    • Clonality

      Monoclonal
    • Clone number

      EPR12763
    • Isotype

      IgG
    • Research areas

      • Neuroscience
      • Cell Type Marker
      • Neuron marker
      • Soma marker
      • Tags & Cell Markers
      • Cell Type Markers
      • Neuroscience Markers
      • Neuronal
      • Epigenetics and Nuclear Signaling
      • Transcription
      • Domain Families
      • Forkhead Box
      • Other FOXes
      • Epigenetics and Nuclear Signaling
      • Transcription
      • Transcription Factors

    Associated products

    • Alternative Versions

      • Anti-NeuN antibody [EPR12763] - BSA and Azide free (ab209898)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab306540 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt (Intra)
    1/2500.
    Notes
    Flow Cyt (Intra)
    1/2500.

    Target

    • Function

      RNA-binding protein that regulates alternative splicing events.
    • Sequence similarities

      Contains 1 RRM (RNA recognition motif) domain.
    • Cellular localization

      Nucleus. Cytoplasm.
    • Target information above from: UniProt accession A6NFN3 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 52897 Mouse
      • SwissProt: Q8BIF2 Mouse
      • Unigene: 341103 Mouse
      • Alternative names

        • FLJ56884 antibody
        • FLJ58356 antibody
        • Fox-1 homolog C antibody
        • fox1 homolog C antibody
        • Fox3 antibody
        • FOX3NeuN antibody
        • hexaribonucleotide binding protein 3 antibody
        • HRNBP3 antibody
        • NEUN antibody
        • neuronal nuclei antibody
        • Rbfox3 antibody
        • RFOX3_HUMAN antibody
        • RNA binding protein fox-1 homolog 3 antibody
        • RNA binding protein, fox 1 homolog (C. elegans) 3 antibody
        see all

      Images

      • Flow Cytometry (Intracellular) - PE Anti-NeuN antibody [EPR12763] (Neuronal Marker) (ab306540)
        Flow Cytometry (Intracellular) - PE Anti-NeuN antibody [EPR12763] (Neuronal Marker) (ab306540)

        Flow cytometry overlay histogram showing Neuro 2a cells stained with ab306540 (red line). The cells were fixed with 4 % formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were incubated in 1x PBS containing 10 % normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab306540) (1x 106 in 100μl at 0.04μg/ml (1/12500)) for 30 min at 22°C.

        Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

        Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter.

        This antibody gave a positive signal in Neuro 2a cells fixed with 80 % methanol (5 min) / permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (0)

      Publishing research using ab306540? Please let us know so that we can cite the reference in this datasheet.

      ab306540 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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