Recombinant PE Anti-NeuN antibody [EPR12763] (Neuronal Marker) (ab306540)

Flow cytometry overlay histogram showing Neuro 2a cells stained with ab306540 (red line). The cells were fixed with 4 % formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were incubated in 1x PBS containing 10 % normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab306540) (1x 10⁶ in 100μl at 0.04μg/ml (1/12500)) for 30 min at 22°C.

Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter.

This antibody gave a positive signal in Neuro 2a cells fixed with 80 % methanol (5 min) / permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.