Recombinant PE Anti-PD-L1 antibody [28-8] (ab209962)
![Flow Cytometry - PE Anti-PD-L1 antibody [28-8] (ab209962)](https://www.abcam.com/ps/products/209/ab209962/Images/ab209962-265295-anti-pd-l1-antibody-28-8-phycoerythrin-flow-cytometry.jpg "Flow Cytometry - PE Anti-PD-L1 antibody [28-8] (ab209962)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PE Rabbit monoclonal [28-8] to PD-L1
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
Related conjugates and formulations
Overview
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Product name
PE Anti-PD-L1 antibody [28-8]
See all PD-L1 primary antibodies -
Description
PE Rabbit monoclonal [28-8] to PD-L1 -
Host species
Rabbit -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Fusion protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow Cyt: CHO-PDL1 cells
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General notes
Anti-PD-L1 antibody [28-8] has been used as detector antibody in Human PD-L1 SimpleStep ELISA® kit (ab214565).
Additional information on positive controls:
Cell Lines:
Positives: B-CPAP- high, ES-2- medium, HCC70 - lowFor primary negative control, isotype control, RabMAb negative control antibody (ab172730) is recommended.
For negative control sample, cell line COLO205 is recommended.
For PD-L1 protein, see ab167713.
Recommended protocols:
For recommended Flow Cytometry (Flow Cyt) protocol, please refer to the protocol book (line 1) in the protocol section and/or here (downloadable copy)
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark. -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
28-8 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-PD-L1 antibody [28-8] (ab205921)
- APC Anti-PD-L1 antibody [28-8] (ab206967)
- Anti-PD-L1 antibody [28-8] - Low endotoxin, Azide free (ab209889)
- Alexa Fluor® 488 Anti-PD-L1 antibody [28-8] (ab209959)
- Alexa Fluor® 647 Anti-PD-L1 antibody [28-8] - Extracellular domain (ab209960)
- HRP Anti-PD-L1 antibody [28-8] (ab209961)
- Alexa Fluor® 555 Anti-PD-L1 antibody [28-8] (ab213358)
- Alexa Fluor® 568 Anti-PD-L1 antibody [28-8] (ab213359)
- Alexa Fluor® 594 Anti-PD-L1 antibody [28-8] (ab213360)
- FITC Anti-PD-L1 antibody [28-8] (ab224027)
- Anti-PD-L1 antibody [28-8] - BSA and Azide free (ab228413)
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab209962 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
1/100.
ab209478 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. The cellular localisation of this product has been verified in ICC/IF. |
| Notes |
|---|
|
Flow Cyt
1/100. ab209478 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. The cellular localisation of this product has been verified in ICC/IF. |
Target
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Function
Involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production. -
Tissue specificity
Highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes. -
Sequence similarities
Belongs to the immunoglobulin superfamily. BTN/MOG family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Cell membrane and Endomembrane system. - Information by UniProt
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Database links
- Entrez Gene: 29126 Human
- Omim: 605402 Human
- SwissProt: Q9NZQ7 Human
- Unigene: 521989 Human
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Alternative names
- B7 H antibody
- B7 H1 antibody
- B7 homolog 1 antibody
see all
Images
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Flow Cytometry - PE Anti-PD-L1 antibody [28-8] (ab209962)
Overlay histgram showing CHO (blue line) and CHO-PD-L1 transfected (red line) cells stained with ab209962.
The cells were incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab209962, 1/100 dilution) for 30 min at 4ºC.
Acquisition of >5,000 events were collected using a 20 mW Solid State Blue Laser (488nm) and 585/42 bandpass filter.
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![PE Anti-PD-L1 antibody [28-8] (ab209962)](https://www.abcam.com/ps/products/209/ab209962/Images/ab209962-3-benefits-of-recombinant-antibodies.png)
PE Anti-PD-L1 antibody [28-8] (ab209962)
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab209962 has been referenced in 1 publication.
- Jia Q et al. Lung cancer cells expressing a shortened CDK16 3'UTR escape senescence through impaired miR-485-5p targeting. Mol Oncol 16:1347-1364 (2022). PubMed: 34687270