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RecombinantRabMAb

Recombinant PE Anti-SIRP alpha antibody [EPR16264] (ab213195)

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  • Certificate of Compliance
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Flow Cytometry (Intracellular) - PE Anti-SIRP alpha antibody [EPR16264] (ab213195)
  • PE Anti-SIRP alpha antibody [EPR16264] (ab213195)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • PE Rabbit monoclonal [EPR16264] to SIRP alpha
  • Suitable for: Flow Cyt (Intra)
  • Reacts with: Rat
  • Conjugation: PE. Ex: 488nm, Em: 575nm

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free Unconjugated

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Isotype
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Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199093)
Primary
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Anti-SIRP alpha antibody [EPR16264] - Low endotoxin, Azide free (ab209892)
Protein
Product image
Recombinant human SIRP alpha protein (Fc Chimera Active) (ab221342)

View more associated products

Overview

  • Product name

    PE Anti-SIRP alpha antibody [EPR16264]
    See all SIRP alpha primary antibodies
  • Description

    PE Rabbit monoclonal [EPR16264] to SIRP alpha
  • Host species

    Rabbit
  • Conjugation

    PE. Ex: 488nm, Em: 575nm
  • Tested applications

    Suitable for: Flow Cyt (Intra)more details
  • Species reactivity

    Reacts with: Rat
    Predicted to work with: Mouse, Human
  • Immunogen

    Recombinant fragment within Human SIRP alpha aa 350-500. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: P78324

  • Positive control

    • Flow Cyt (intra): PC12 cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark.
  • Storage buffer

    pH: 7.4
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR16264
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Cell Adhesion Proteins
    • ECM Proteins
    • Neuroscience
    • Neurology process
    • Neural Signal Transduction
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Synapse marker
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Inflammatory mediators

Associated products

  • Alternative Versions

    • Anti-SIRP alpha antibody [EPR16264] (ab191419)
    • Anti-SIRP alpha antibody [EPR16264] - Low endotoxin, Azide free (ab209892)
    • Alexa Fluor® 647 Anti-SIRP alpha antibody [EPR16264] (ab214274)
    • Alexa Fluor® 488 Anti-SIRP alpha antibody [EPR16264] (ab302974)
  • Isotype control

    • PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)
  • Recombinant Protein

    • Recombinant human SIRP alpha protein (Fc Chimera Active) (ab221342)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab213195 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/500.

The cellular localisation of this product has been verified in ICC/IF.

Notes
Flow Cyt (Intra)
1/500.

The cellular localisation of this product has been verified in ICC/IF.

Target

  • Function

    Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function (By similarity). Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.
  • Tissue specificity

    Ubiquitous. Highly expressed in brain. Detected on myeloid cells, but not T-cells. Detected at lower levels in heart, placenta, lung, testis, ovary, colon, liver, small intestine, prostate, spleen, kidney, skeletal muscle and pancreas.
  • Sequence similarities

    Contains 2 Ig-like C1-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications

    N-glycosylated.
    Phosphorylated on tyrosine residues in response to stimulation with EGF, growth hormone, insulin and PDGF. Dephosphorylated by PTPN11.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P78324 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 140885 Human
    • Entrez Gene: 19261 Mouse
    • Entrez Gene: 25528 Rat
    • Omim: 602461 Human
    • SwissProt: P78324 Human
    • SwissProt: P97797 Mouse
    • SwissProt: P97710 Rat
    • Unigene: 581021 Human
    • Unigene: 1682 Mouse
    • Unigene: 53971 Rat
    see all
  • Alternative names

    • Signal regulatory protein alpha type 1 antibody
    • Bit antibody
    • Brain Ig like molecule with tyrosine based activation motifs antibody
    • Brain Ig-like molecule with tyrosine-based activation motifs antibody
    • Brain immunoglobulin like molecule with tyrosine based activation motifs antibody
    • CD172 antigen like family member A antibody
    • CD172 antigen-like family member A antibody
    • CD172a antibody
    • CD172a antigen antibody
    • Inhibitory receptor SHPS-1 antibody
    • Macrophage fusion receptor antibody
    • MFR antibody
    • MYD 1 antibody
    • Myd 1 antigen antibody
    • MyD-1 antigen antibody
    • p84 antibody
    • Protein tyrosine phosphatase non receptor type substrate 1 antibody
    • PTPNS1 antibody
    • SHP substrate 1 antibody
    • SHPS-1 antibody
    • SHPS1 antibody
    • SHPS1_HUMAN antibody
    • Signal regulatory protein alpha 2 antibody
    • Signal regulatory protein alpha 3 antibody
    • Signal regulatory protein alpha antibody
    • Signal regulatory protein alpha type 2 antibody
    • Signal-regulatory protein alpha-1 antibody
    • Signal-regulatory protein alpha-2 antibody
    • Signal-regulatory protein alpha-3 antibody
    • SIRP antibody
    • Sirp-alpha-1 antibody
    • Sirp-alpha-2 antibody
    • Sirp-alpha-3 antibody
    • SIRPA antibody
    • SIRPalpha antibody
    • SIRPalpha1 antibody
    • SIRPalpha2 antibody
    • SIRPalpha3 antibody
    • Tyrosine phosphatase SHP substrate 1 antibody
    • Tyrosine protein phosphatase non receptor type substrate 1 antibody
    • Tyrosine-protein phosphatase non-receptor type substrate 1 antibody
    see all

Images

  • Flow Cytometry (Intracellular) - PE Anti-SIRP alpha antibody [EPR16264] (ab213195)
    Flow Cytometry (Intracellular) - PE Anti-SIRP alpha antibody [EPR16264] (ab213195)

    Overlay histogram showing PC12 cells stained with ab213195 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab213195, 1/500 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50mW Yellow/Green laser (561nm) and 586/15 bandpass filter.

    This antibody gave a positive signal in PC12 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

  • PE Anti-SIRP alpha antibody [EPR16264] (ab213195)
    PE Anti-SIRP alpha antibody [EPR16264] (ab213195)

Protocols

  • Flow cytometry protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (0)

Publishing research using ab213195? Please let us know so that we can cite the reference in this datasheet.

ab213195 has not yet been referenced specifically in any publications.

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