PE Anti-TNF Receptor I antibody [H398] (ab270654)
Key features and details
- PE Mouse monoclonal [H398] to TNF Receptor I
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG2a
Related conjugates and formulations
Overview
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Product name
PE Anti-TNF Receptor I antibody [H398]
See all TNF Receptor I primary antibodies -
Description
PE Mouse monoclonal [H398] to TNF Receptor I -
Host species
Mouse -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human TNF Receptor I.
Database link: P19438 -
Positive control
- Flow: Human peripheral blood and human monocytes.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Size exclusion -
Clonality
Monoclonal -
Clone number
H398 -
Isotype
IgG2a -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab270654 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt |
Use 10µl for 106 cells.
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Notes |
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Flow Cyt
Use 10µl for 106 cells. |
Target
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Function
Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase. -
Involvement in disease
Familial hibernian fever
Multiple sclerosis 5 -
Sequence similarities
Contains 1 death domain.
Contains 4 TNFR-Cys repeats. -
Domain
The domain that induces A-SMASE is probably identical to the death domain. The N-SMASE activation domain (NSD) is both necessary and sufficient for activation of N-SMASE.
Both the cytoplasmic membrane-proximal region and the C-terminal region containing the death domain are involved in the interaction with TRPC4AP. -
Post-translational
modificationsThe soluble form is produced from the membrane form by proteolytic processing. -
Cellular localization
Cell membrane. Golgi apparatus membrane. Secreted. A secreted form is produced through proteolytic processing and Secreted. Lacks a Golgi-retention motif, is not membrane bound and therefore is secreted. - Information by UniProt
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Database links
- Entrez Gene: 7132 Human
- Omim: 191190 Human
- SwissProt: P19438 Human
- Unigene: 279594 Human
- Unigene: 713833 Human
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Alternative names
- CD120a antibody
- FPF antibody
- MGC19588 antibody
see all
Images
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Flow cytometry analysis of human monocytes (red) and lymphocytes (black) cells labeling CD120a using ab270654. Concentration of 10 μl reagent/100μl peripheral whole blood.
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Flow cytometric analysis of human peripheral whole blood cells labeling TNF Receptor I using ab270654. The concentration of 10 μl reagent/ 100µl of peripheral whole blood.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab270654 has not yet been referenced specifically in any publications.