Recombinant Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] - BSA and Azide free (ab226866)
![Immunoprecipitation - Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] - BSA and Azide free (ab226866)](https://www.abcam.com/ps/products/226/ab226866/Images/ab226866-321771-anti-pentraxin-3-ptx3-antibody-epr18678-105-immunoprecipitation.jpg "Immunoprecipitation - Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] - BSA and Azide free (ab226866)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18678-105] to Pentraxin 3/PTX3 - BSA and Azide free
- Suitable for: Flow Cyt (Intra), IP, ICC/IF, WB
- Reacts with: Human, Recombinant fragment
Related conjugates and formulations
Overview
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Product name
Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] - BSA and Azide free
See all Pentraxin 3/PTX3 primary antibodies -
Description
Rabbit monoclonal [EPR18678-105] to Pentraxin 3/PTX3 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), IP, ICC/IF, WBmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: HUVEC cells.
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General notes
ab226866 is the carrier-free version of ab190838.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18678-105 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab226866 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
Use at an assay dependent concentration.
|
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| IP |
Use at an assay dependent concentration.
|
|
| ICC/IF |
Use at an assay dependent concentration.
|
|
| WB |
Use at an assay dependent concentration. Detects a band of approximately 42 kDa (predicted molecular weight: 41 kDa).
|
| Notes |
|---|
|
Flow Cyt (Intra)
Use at an assay dependent concentration. |
|
IP
Use at an assay dependent concentration. |
|
ICC/IF
Use at an assay dependent concentration. |
|
WB
Use at an assay dependent concentration. Detects a band of approximately 42 kDa (predicted molecular weight: 41 kDa). |
Target
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Function
Plays a role in the regulation of innate resistance to pathogens, inflammatory reactions, possibly clearance of self-components and female fertility. -
Sequence similarities
Contains 1 pentaxin domain. -
Post-translational
modificationsGlycosylated. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 5806 Human
- Omim: 602492 Human
- SwissProt: P26022 Human
- Unigene: 591286 Human
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Alternative names
- AI607804 antibody
- Pentaxin 3 antibody
- Pentaxin-related protein PTX3 antibody
see all
Images
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Immunoprecipitation - Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] - BSA and Azide free (ab226866)
Pentraxin 3/PTX3 was immunoprecipitated from 0.35 mg of HUVEC (human umbilical vein endothelial cell line) whole cell lysate with ab190838 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab190838 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1: HUVEC whole cell lysate 10 μg (Input).
Lane 2: ab190838 IP in HUVEC whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab190838 in HUVEC whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.The molecular weight observed is consistent with what has been described in the literature (PMID: 17389238).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab190838).
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![Flow Cytometry (Intracellular) - Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] - BSA and Azide free (ab226866)](https://www.abcam.com/ps/products/226/ab226866/Images/ab226866-321770-anti-pentraxin-3-ptx3-antibody-epr18678-105-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] - BSA and Azide free (ab226866)Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HUVEC (human umbilical vein endothelial cell line) cells labeling Pentraxin 3/PTX3 with ab190838 at 1/60 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab190838).
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![Immunocytochemistry/ Immunofluorescence - Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] - BSA and Azide free (ab226866)](https://www.abcam.com/ps/products/226/ab226866/Images/ab226866-288282-anti-pentraxin-3-ptx3-antibody-epr18678-105-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] - BSA and Azide free (ab226866)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HUVEC (human umbilical vein endothelial cell line) cells labeling Pentraxin 3/PTX3 with ab190838 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HUVEC cell line.
The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) secondary antibody at 1/200 dilution (red).
-ve control: PBS only, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab190838).
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![Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] - BSA and Azide free (ab226866)](https://www.abcam.com/ps/products/226/ab226866/Images/ab226866-4-benefits-of-recombinant-antibodies.png)
Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] - BSA and Azide free (ab226866)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab226866 has not yet been referenced specifically in any publications.
