Anti-PEP1 antibody [18C8BC7AD10] (ab113690)
![Western blot - Anti-PEP1 antibody [18C8BC7AD10] (ab113690)](https://www.abcam.com/ps/products/113/ab113690/Images/ab113690-126384-anti-pep1-antibody-18c8bc7ad10-western-blot.jpg "Western blot - Anti-PEP1 antibody [18C8BC7AD10] (ab113690)")
Key features and details
- Mouse monoclonal [18C8BC7AD10] to PEP1
- Suitable for: WB
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG2c
Overview
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Product name
Anti-PEP1 antibody [18C8BC7AD10] -
Description
Mouse monoclonal [18C8BC7AD10] to PEP1 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Saccharomyces cerevisiae -
Immunogen
Recombinant full length protein. This information is considered to be commercially sensitive.
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Positive control
- Yeast lysate
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
Purity
Proprietary Purification -
Purification notes
Near homogeneity (Purity >95% by SDS-PAGE). The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by chemical fractionation. -
Clonality
Monoclonal -
Clone number
18C8BC7AD10 -
Isotype
IgG2c -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab113690 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 4 µg/ml. Detects a band of approximately 177 kDa (predicted molecular weight: 177 kDa).
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| Notes |
|---|
|
WB
Use a concentration of 4 µg/ml. Detects a band of approximately 177 kDa (predicted molecular weight: 177 kDa). |
Target
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Function
Functions as a sorting receptor in the Golgi compartment required for the intracellular sorting and delivery of soluble vacuolar proteins, like carboxypeptidase Y (CPY) and proteinase A. May execute multiple rounds of sorting by cycling between the late Golgi and a prevacuolar endosome-like compartment. Binds the Golgi-modified P2 form of CPY, and this interaction is dependent on the presence of an intact CPY vacuolar protein sorting signal. -
Sequence similarities
Belongs to the VPS10-related sortilin family.
Contains 10 BNR repeats. -
Domain
The lumenal domain contains two regions of approximately 650 AA that exhibit 20% identity. The cytoplasmic domain serves as a Golgi retention/recycling signal. -
Cellular localization
Golgi apparatus > trans-Golgi network membrane. Prevacuolar compartment membrane. Cycles between the Golgi apparatus and the prevacuolar compartments. - Information by UniProt
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Database links
- Entrez Gene: 852264 Saccharomyces cerevisiae
- SwissProt: P32319 Saccharomyces cerevisiae
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Alternative names
- Carboxypeptidase Y receptor antibody
- Carboxypeptidase Y-deficient protein 1 antibody
- CPY receptor antibody
see all
Images
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Western blot - Anti-PEP1 antibody [18C8BC7AD10] (ab113690)Anti-PEP1 antibody [18C8BC7AD10] (ab113690) + Yeast whole cell lysate at 15 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 177 kDa
Datasheets and documents
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Datasheet download
References (2)
ab113690 has been referenced in 2 publications.
- Nightingale DJH et al. A Protocol to Map the Spatial Proteome Using HyperLOPIT in Saccharomyces cerevisiae. Bio Protoc 9:e3303 (2019). PubMed: 33654815
- Aufschnaiter A et al. The Coordinated Action of Calcineurin and Cathepsin D Protects Against a-Synuclein Toxicity. Front Mol Neurosci 10:207 (2017). PubMed: 28713240
