Anti-PEP12 antibody [2C3G4] (ab113689)
![Western blot - Anti-PEP12 antibody [2C3G4] (ab113689)](https://www.abcam.com/ps/products/113/ab113689/Images/ab113689-126383-anti-pep12-antibody-2c3g4-western-blot.jpg "Western blot - Anti-PEP12 antibody [2C3G4] (ab113689)")
Key features and details
- Mouse monoclonal [2C3G4] to PEP12
- Suitable for: WB, ICC/IF
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG1
Overview
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Product name
Anti-PEP12 antibody [2C3G4] -
Description
Mouse monoclonal [2C3G4] to PEP12 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Saccharomyces cerevisiae -
Immunogen
Recombinant full length protein. This information is considered to be commercially sensitive.
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Positive control
- Yeast lysate
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
Purity
Proprietary Purification -
Purification notes
Near homogeneity (Purity >95% by SDS-PAGE). The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by chemical fractionation. -
Clonality
Monoclonal -
Clone number
2C3G4 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab113689 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
|
|
| ICC/IF |
Use a concentration of 1 µg/ml.
|
| Notes |
|---|
|
WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa). |
|
ICC/IF
Use a concentration of 1 µg/ml. |
Target
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Function
Plays a role in the sorting and targeting of vacuolar proteases. -
Sequence similarities
Belongs to the syntaxin family.
Contains 1 t-SNARE coiled-coil homology domain. -
Post-translational
modificationsUbiquitinated. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 854201 Saccharomyces cerevisiae
- SwissProt: P32854 Saccharomyces cerevisiae
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Alternative names
- Carboxypeptidase Y-deficient protein 12 antibody
- PEP12 antibody
- PEP12_YEAST antibody
see all
Images
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Western blot - Anti-PEP12 antibody [2C3G4] (ab113689)Anti-PEP12 antibody [2C3G4] (ab113689) + Yeast whole cell lysate at 15 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 33 kDa
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab113689 has not yet been referenced specifically in any publications.
