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RecombinantRabMAb

Recombinant Anti-Pepsinogen II/PGC antibody [EPR22843-269] - BSA and Azide free (ab259997)

Submit a review Submit a question References (1)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pepsinogen II/PGC antibody [EPR22843-269] - BSA and Azide free (ab259997)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pepsinogen II/PGC antibody [EPR22843-269] - BSA and Azide free (ab259997)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pepsinogen II/PGC antibody [EPR22843-269] - BSA and Azide free (ab259997)
  • Immunohistochemistry (Frozen sections) - Anti-Pepsinogen II/PGC antibody [EPR22843-269] - BSA and Azide free (ab259997)
  • Immunohistochemistry (Frozen sections) - Anti-Pepsinogen II/PGC antibody [EPR22843-269] - BSA and Azide free (ab259997)
  • Anti-Pepsinogen II/PGC antibody [EPR22843-269] - BSA and Azide free (ab259997)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22843-269] to Pepsinogen II/PGC - BSA and Azide free
  • Suitable for: WB, IHC-P, IHC-Fr
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

Overview

  • Product name

    Anti-Pepsinogen II/PGC antibody [EPR22843-269] - BSA and Azide free
    See all Pepsinogen II/PGC primary antibodies

  • Description

    Rabbit monoclonal [EPR22843-269] to Pepsinogen II/PGC - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IHC-Frmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: His-tagged Recombinant Human Pepsinogen C/PGC protein, Human stomach, Human testis, MDA-MB-231, Rat stomach and Mouse stomach lysates. IHC-P: Human stomach, Mouse stomach and Rat stomach tissues.

  • General notes

    ab259997 is the carrier-free version of ab255826.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab259997 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 32, 38 kDa (predicted molecular weight: 42 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr
Use at an assay dependent concentration.
Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 32, 38 kDa (predicted molecular weight: 42 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr
Use at an assay dependent concentration.
Application notes
Is unsuitable for IP.

Target

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pepsinogen II/PGC antibody [EPR22843-269] - BSA and Azide free (ab259997)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pepsinogen II/PGC antibody [EPR22843-269] - BSA and Azide free (ab259997)

    Immunohistochemical analysis of paraffin-embedded Rat stomach tissue labeling Pepsinogen II/PGC with ab255826 at 1/5000 dilution (0.129 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat stomach. The section was incubated with ab255826 for 10 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control/ Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255826).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pepsinogen II/PGC antibody [EPR22843-269] - BSA and Azide free (ab259997)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pepsinogen II/PGC antibody [EPR22843-269] - BSA and Azide free (ab259997)

    Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling Pepsinogen II/PGC with ab255826 at 1/5000 dilution (0.129 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse stomach. The section was incubated with ab255826 for 10 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control/ Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255826).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pepsinogen II/PGC antibody [EPR22843-269] - BSA and Azide free (ab259997)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pepsinogen II/PGC antibody [EPR22843-269] - BSA and Azide free (ab259997)

    Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling Pepsinogen II/PGC with ab255826 at 1/5000 dilution (0.129 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human stomach (PMID 10883891). The section was incubated with ab255826 for 10 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control/ Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255826).

  • Immunohistochemistry (Frozen sections) - Anti-Pepsinogen II/PGC antibody [EPR22843-269] - BSA and Azide free (ab259997)
    Immunohistochemistry (Frozen sections) - Anti-Pepsinogen II/PGC antibody [EPR22843-269] - BSA and Azide free (ab259997)

    Immunohistochemical analysis of frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized rat stomach tissue labeling Annexin-4/ANXA4 with ab255826 at 1/500 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution (green). Positive staining on rat gastric mucosa (PMID: 10883891) is observed. The nuclear counter stain is DAPI (blue).
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255826).

  • Immunohistochemistry (Frozen sections) - Anti-Pepsinogen II/PGC antibody [EPR22843-269] - BSA and Azide free (ab259997)
    Immunohistochemistry (Frozen sections) - Anti-Pepsinogen II/PGC antibody [EPR22843-269] - BSA and Azide free (ab259997)

    Immunohistochemical analysis of frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized mouse stomach tissue labeling Annexin-4/ANXA4 with ab255826 at 1/500 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution (green). Positive staining on mouse gastric mucosa (PMID: 10883891) is observed. The nuclear counter stain is DAPI (blue).
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255826).

  • Anti-Pepsinogen II/PGC antibody [EPR22843-269] - BSA and Azide free (ab259997)
    Anti-Pepsinogen II/PGC antibody [EPR22843-269] - BSA and Azide free (ab259997)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (1)

Publishing research using ab259997? Please let us know so that we can cite the reference in this datasheet.

ab259997 has been referenced in 1 publication.

  • Mao FY  et al. Helicobacter pylori-Induced Rev-erbα Fosters Gastric Bacteria Colonization by Impairing Host Innate and Adaptive Defense. Cell Mol Gastroenterol Hepatol 12:395-425 (2021). PubMed: 33676046

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