PerCP/Cy5.5® Mouse IgG2b, kappa monoclonal [MPC-11] - Isotype Control (ab239460)
![Flow Cytometry - PerCP/Cy5.5® Mouse IgG2b, kappa monoclonal [MPC-11] - Isotype Control (ab239460)](https://www.abcam.com/ps/products/239/ab239460/Images/ab239460-317757-mouse-igg2b-kappa-monoclonal-mpc-11-percp-cy55-isotype-control-flow-cytometry.jpg "Flow Cytometry - PerCP/Cy5.5® Mouse IgG2b, kappa monoclonal [MPC-11] - Isotype Control (ab239460)")
Related conjugates and formulations
Overview
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Product name
PerCP/Cy5.5® Mouse IgG2b, kappa monoclonal [MPC-11] - Isotype Control -
Conjugation
PerCP/Cy5.5®. Ex: 482nm, Em: 690nm -
Specificity
This mouse IgG2b (kappa) monoclonal antibody (clone MPC-11) reacts with an epitope irrelevant for a variety of resting, activated, live, and fixed human, mouse, and rat tissues. -
Tested applications
Suitable for: Flow Cytmore details -
Immunogen
Chemical/ Small Molecule conjugated to keyhole limpet haemocyanin. (KLH-coupled trinitrophenol).
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General notes
This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy® and CyDye® are trademarks of Cytiva.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.4
Preservative: 0.0975% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Size exclusion -
Purification notes
Purified antibody is conjugated with PerCP/Cy5.5® under optimum conditions. The conjugate is purified by size-exclusion chromatography. -
Clonality
Monoclonal -
Clone number
MPC-11 -
Isotype
IgG2b -
Light chain type
kappa
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab239460 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use at an assay dependent concentration.
The reagent is intended as isotype control for flow cytometry analysis to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this isotype control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 µg per ml of incubation mixture), change the conditions of your experiment to reduce the background. |
| Notes |
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Flow Cyt
Use at an assay dependent concentration. The reagent is intended as isotype control for flow cytometry analysis to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this isotype control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 µg per ml of incubation mixture), change the conditions of your experiment to reduce the background. |
Images
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Flow Cytometry - PerCP/Cy5.5® Mouse IgG2b, kappa monoclonal [MPC-11] - Isotype Control (ab239460)
Example of nonspecific ab239460 signal on human peripheral blood; surface staining, 1 μg/ml.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab239460 has not yet been referenced specifically in any publications.
