Recombinant Anti-Peregrin/BRPF1 antibody [EPR24069-57] (ab259840)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24069-57] to Peregrin/BRPF1
- Suitable for: Flow Cyt (Intra), ICC/IF, WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Peregrin/BRPF1 antibody [EPR24069-57]
See all Peregrin/BRPF1 primary antibodies -
Description
Rabbit monoclonal [EPR24069-57] to Peregrin/BRPF1 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), ICC/IF, WBmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK-293T, HeLa, 293T, NIH/3T3, PC-12 and His-tagged human BRPF1 recombinant protein lysates. ICC/IF: HeLa, NIH/3T3 cells. Flow Cyt: HeLa, 4T1 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR24069-57 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab259840 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/50.
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ICC/IF |
1/50.
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WB |
1/1000. Predicted molecular weight: 137 kDa.
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Notes |
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Flow Cyt (Intra)
1/50. |
ICC/IF
1/50. |
WB
1/1000. Predicted molecular weight: 137 kDa. |
Target
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Function
Component of the MOZ/MORF complex which has a histone H3 acetyltransferase activity. Positively regulates the transcription of RUNX1 and RUNX2. -
Tissue specificity
High levels in testis. -
Sequence similarities
Contains 1 bromo domain.
Contains 1 C2H2-type zinc finger.
Contains 1 PHD-type zinc finger.
Contains 1 PWWP domain. -
Post-translational
modificationsAcetylated by KAT6A. -
Cellular localization
Nucleus. Cytoplasm. Localization to the nucleus depends on KAT6A, ING5 and MEAF6. - Information by UniProt
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Database links
- Entrez Gene: 7862 Human
- Entrez Gene: 78783 Mouse
- Entrez Gene: 309647 Rat
- Omim: 602410 Human
- SwissProt: P55201 Human
- Unigene: 1004 Human
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Alternative names
- BR140 antibody
- Bromodomain and PHD finger containing 1 antibody
- Bromodomain and PHD finger-containing protein 1 antibody
see all
Images
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Lanes 1-3 & 5-6 : Anti-Peregrin/BRPF1 antibody [EPR24069-57] (ab259840) at 1/500 dilution
Lane 1 : Wild-type HEK-293T cell lysate at 20 µg
Lanes 2-3 : BRPF1 knockout HEK-293T cell lysate at 20 µg
Lane 4 : Empty
Lane 5 : PC-3 cell lysate at 20 µg
Lane 6 : HepG2 cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 137 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-Peregrin/BRPF1 antibody [EPR24069-57] staining at 1/500 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab259840 was shown to bind specifically to Peregrin/BRPF1. A band was observed at 120 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in BRPF1 knockout cell line ab266549 (knockout cell lysate ab258795). To generate this image, wild-type and BRPF1 knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
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All lanes : Anti-Peregrin/BRPF1 antibody [EPR24069-57] (ab259840) at 1/1000 dilution
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : 293T (human embryonic kidney epithelial cell) whole cell lysate
Lane 3 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lane 4 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 137 kDa
Observed band size: 150-160 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
The molecular weight observed is consistent with what has been described in the literature (PMID: 7906940).
Lysates were made freshly and used in WB immediately to minimize protein degradation.
Exposure time: 3 minutes
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All lanes : Anti-Peregrin/BRPF1 antibody [EPR24069-57] (ab259840) at 1/5000 dilution
Lane 1 : His-tagged human BRPF1 recombinant protein at 0.01 µg
Lane 2 : His-tagged human BRPF3 recombinant protein at 0.2 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 137 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 8 seconds
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Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized HeLa cells labelling Peregrin/BRPF1 with ab259840 at 1/50 (11.34 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in HeLa cells is observed.
ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.
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Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 cells labelling Peregrin/BRPF1 with ab259840 at 1/50 (11.34 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in NIH/3T3 cells is observed.
ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.
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Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma cell) cells labelling Peregrin/BRPF1 with ab259840 at 1/500 dilution (0.1µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
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Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized 4T1 (mouse mammary gland carcinoma epithelial cell) cells labelling Peregrin/BRPF1 with ab259840 at 1/500 dilution (0.1µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab259840 has been referenced in 1 publication.
- Lin G et al. Deubiquitinase USP35 stabilizes BRPF1 to activate mevalonate (MVA) metabolism during prostate tumorigenesis. Cell Death Discov 8:453 (2022). PubMed: 36357379