Recombinant Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker (ab28144)
![Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker (ab28144)](https://www.abcam.com/ps/products/28/ab28144/Images/ab28144-413145-anti-pericentrin-antibody-mabcam-28144-centrosome-marker-immunocytochemistry-hela-human.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker (ab28144)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [mAbcam 28144] to Pericentrin - Centrosome Marker
- Suitable for: ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker
See all Pericentrin primary antibodies -
Description
Mouse monoclonal [mAbcam 28144] to Pericentrin - Centrosome Marker -
Host species
Mouse -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chinese hamster
Does not react with:
Mouse -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: HeLa cells.
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General notes
This product has switched from a hybridoma to recombinant production method on 9th September 2020.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
mAbcam 28144 -
Myeloma
Sp2/0 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab28144 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | (11) |
1/50.
|
| Notes |
|---|
|
ICC/IF
1/50. |
Target
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Function
Integral component of the filamentous matrix of the centrosome involved in the initial establishment of organized microtubule arrays in both mitosis and meiosis. Plays a role, together with DISC1, in the microtubule network formation. Is an integral component of the pericentriolar material (PCM). May play an important role in preventing premature centrosome splitting during interphase by inhibiting NEK2 kinase activity at the centrosome. -
Tissue specificity
Expressed in all tissues tested, including placenta, liver, kidney and thymus. -
Involvement in disease
Microcephalic osteodysplastic primordial dwarfism 2 -
Domain
Composed of a coiled-coil central region flanked by non-helical N- and C-terminals. -
Cellular localization
Cytoplasm > cytoskeleton > microtubule organizing center > centrosome. Centrosomal at all stages of the cell cycle. Remains associated with centrosomes following microtubule depolymerization. Colocalized with DISC1 at the centrosome. - Information by UniProt
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Database links
- Entrez Gene: 5116 Human
- Omim: 605925 Human
- SwissProt: O95613 Human
- Unigene: 474069 Human
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Alternative names
- Centrosome Marker antibody
- Ken antibody
- Kendrin antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker (ab28144)
Immunocytochemical analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Pericentrin with ab28144 at 1/50 dilution at 4°C overnight, followed by AlexaFluor® 488-conjugated Goat anti-Rabbit secondary
antibody (ab150077) at 1/1000 dilution at RT for 30min. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] (ab195889) was used as a counterstain at 1/200 dilution at RT for 30min. Nucleus were visualized using DAPI. The cells were 4% PFA fixed and permeabilized with 0.1% Triton X-100.Confocal image showing centrosome staining in HeLa cells.
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![Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker (ab28144)](https://www.abcam.com/ps/products/28/ab28144/Images/ab28144-275774-anti-pericentrin-antibody-mabcam-28144-centrosome-marker-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker (ab28144)Image from Sikkema, William K. A. et al. PLoS ONE 9.6 (2014): e98838. PMC. Web. 24 Feb. 2017. doi: 10.1371/journal.pone.0098838. Fig 4F. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Immunocytochemistry/ Immunofluorescence analysis of Y79 cells labeling Pericentrin with ab28144 (red). Cells were fixed for 10 minutes at room temperature in 4% paraformaldehyde. Cells were then permeabilized using 0.2% Triton/TBS and blocked with 5% NGS in 0.1% BSA/TBS-Tween. The cells were then incubated overnight at 4°C with Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker (ab28144) at 1/1000 diluted in 0.1% BSA/TBS-Tween containing 1% NGS. Cells were then stained for Hoechst (blue) and alpha-tubulin (green).
This image was generated from the hybridoma version.
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![Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker (ab28144)](https://www.abcam.com/ps/products/28/ab28144/Images/ab28144-275773-anti-pericentrin-antibody-mabcam-28144-centrosome-marker-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker (ab28144)This image is courtesy of an anonymous abreview.Immunocytochemistry/ Immunofluorescence analysis of A549 lung cells labeling Pericentrin with ab28144 at 1/100 dilution. Cells were fixed with formaldehyde and permeabilized with Trixton X-100. The cells were blocked with 10% serum for 30 minutes at 20°C, followed by incubation with ab28144 in PBS 10% FCS for 1 hour at 20°C. A polyclonal goat anti-mouse Alexa Fluor® 594 secondary antibody was used at 1/300 dilution.
This image was generated from the hybridoma version. -
![Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker (ab28144)](https://www.abcam.com/ps/products/28/ab28144/Images/ab28144-275770-anti-pericentrin-antibody-mabcam-28144-centrosome-marker-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker (ab28144)This image is courtesy of an Abreview submitted by Dr Kirk McManus, Univ. of Manitoba/Cancer Care MICB.Immunocytochemistry/ Immunofluorescence analysis of HeLa cells labeling Pericentrin with ab28144 at 1/200 dilution. Cells were fixed with methanol, followed by incubation with Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker (ab28144) in PBS for 1 hour at 22°C. A polyclonal goat anti-mouse Cy3® secondary antibody was used at 1/200 dilution. Nuclear counterstained with DAPI.
This image was generated from the hybridoma version.
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![Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker (ab28144)](https://www.abcam.com/ps/products/28/ab28144/Images/ab28144-13-benefits-of-recombinant-antibodies.png)
Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker (ab28144)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (85)
ab28144 has been referenced in 85 publications.
- Meyer N et al. Fetal endothelial colony-forming cell impairment after maternal kidney transplantation. Pediatr Res 93:810-817 (2023). PubMed: 35732823
- Tkach JM et al. Global cellular response to chemical perturbation of PLK4 activity and abnormal centrosome number. Elife 11:N/A (2022). PubMed: 35758262
- Chen F et al. Self-assembly of pericentriolar material in interphase cells lacking centrioles. Elife 11:N/A (2022). PubMed: 35787744
- Morretton JP et al. A catalog of numerical centrosome defects in epithelial ovarian cancers. EMBO Mol Med 14:e15670 (2022). PubMed: 36069081
- Di Paola S et al. PARP10 Mediates Mono-ADP-Ribosylation of Aurora-A Regulating G2/M Transition of the Cell Cycle. Cancers (Basel) 14:N/A (2022). PubMed: 36358629