Recombinant Anti-Periostin antibody [EPR19934] (ab219056)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR19934] (ab219056)](https://www.abcam.com/ps/products/219/ab219056/Images/ab219056-279601-anti-periostin-antibody-epr19934-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR19934] (ab219056)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19934] to Periostin
- Suitable for: WB, IHC-P, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Periostin antibody [EPR19934]
See all Periostin primary antibodies -
Description
Rabbit monoclonal [EPR19934] to Periostin -
Host species
Rabbit -
Specificity
This antibody recognizes a different epitope of Periostin compared to ab219057. -
Tested applications
Suitable for: WB, IHC-P, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human fetal heart, fetal kidney and breast cancer lysates. IHC-P: Human colon and lung cancer tissues. IP: Human breast cancer lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19934 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab219056 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 93 kDa (predicted molecular weight: 93 kDa).
|
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| IHC-P |
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| IP |
1/30.
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| Notes |
|---|
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WB
1/1000. Detects a band of approximately 93 kDa (predicted molecular weight: 93 kDa). |
|
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
IP
1/30. |
Target
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Function
Binds to heparin. Induces cell attachment and spreading and plays a role in cell adhesion. May play a role in extracellular matrix mineralization. -
Tissue specificity
Widely expressed with highest levels in aorta, stomach, lower gastrointestinal tract, placenta, uterus and breast. Up-regulated in epithelial ovarian tumors. Not expressed in normal ovaries. Also highly expressed at the tumor periphery of lung carcinoma tissue but not within the tumor. Overexpressed in breast cancers. -
Sequence similarities
Contains 1 EMI domain.
Contains 4 FAS1 domains. -
Post-translational
modificationsGamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 10631 Human
- Omim: 608777 Human
- SwissProt: Q15063 Human
- Unigene: 136348 Human
- Unigene: 721018 Human
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Alternative names
- Fasciclin-I like antibody
- MGC119510 antibody
- MGC119511 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR19934] (ab219056)
Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Periostin with ab219056 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Extracellular matrix staining on human colon (PMID: 26023718).
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR19934] (ab219056)](https://www.abcam.com/ps/products/219/ab219056/Images/ab219056-279600-anti-periostin-antibody-epr19934-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR19934] (ab219056)Immunohistochemical analysis of paraffin-embedded human lung cancer tissue labeling Periostin with ab219056 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Extracellular matrix staining on human lung cancer (PMID: 24273600).
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Western blot - Anti-Periostin antibody [EPR19934] (ab219056)](https://www.abcam.com/ps/products/219/ab219056/Images/ab219056-279603-anti-periostin-antibody-epr19934-western-blot.jpg)
Western blot - Anti-Periostin antibody [EPR19934] (ab219056)All lanes : Anti-Periostin antibody [EPR19934] (ab219056) at 1/1000 dilution
Lane 1 : Human fetal heart tissue lysate
Lane 2 : Human fetal kidney tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 93 kDa
Observed band size: 93 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-Periostin antibody [EPR19934] (ab219056)](https://www.abcam.com/ps/products/219/ab219056/Images/ab219056-279602-anti-periostin-antibody-epr19934-western-blot.jpg)
Western blot - Anti-Periostin antibody [EPR19934] (ab219056)Anti-Periostin antibody [EPR19934] (ab219056) at 1/1000 dilution + Human breast cancer lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 93 kDa
Observed band size: 93 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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![Immunoprecipitation - Anti-Periostin antibody [EPR19934] (ab219056)](https://www.abcam.com/ps/products/219/ab219056/Images/ab219056-279599-anti-periostin-antibody-epr19934-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Periostin antibody [EPR19934] (ab219056)Periostin was immunoprecipitated from 0.35 mg of Human breast cancer lysate with ab219056 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab219056 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: Human breast cancer lysate 10 μg (Input).
Lane 2: ab219056 IP in Human breast cancer lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab219056 in Human breast cancer lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
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![Anti-Periostin antibody [EPR19934] (ab219056)](https://www.abcam.com/ps/products/219/ab219056/Images/ab219056-6-benefits-of-recombinant-antibodies.png)
Anti-Periostin antibody [EPR19934] (ab219056)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab219056 has been referenced in 1 publication.
- Yu B et al. Periostin secreted by cancer-associated fibroblasts promotes cancer stemness in head and neck cancer by activating protein tyrosine kinase 7. Cell Death Dis 9:1082 (2018). PubMed: 30348980