Name: Anti-Peripherin antibody [EPR23445-28]
Brand: RabMAb
SKU: ab246502
Rating: 5 (3 reviews)

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Western blot - Anti-Peripherin antibody [EPR23445-28] (ab246502)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR23445-28] to Peripherin
Suitable for: Flow Cyt (Intra), WB, IP, IHC-P, IHC-Fr, ICC/IF
Reacts with: Mouse, Rat, Human

Alexa Fluor® 488 Alexa Fluor® 594 Alexa Fluor® 647 APC Carrier Free HRP PE

Product name

Anti-Peripherin antibody [EPR23445-28]
See all Peripherin primary antibodies
Description

Rabbit monoclonal [EPR23445-28] to Peripherin
Host species

Rabbit
Tested applications

Suitable for: Flow Cyt (Intra), WB, IP, IHC-P, IHC-Fr, ICC/IFmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human spinal cord, Human lower limb nerve, Human nerve, SH-SY5Y, Mouse dorsal ganglion, Rat dorsal ganglion, Mouse spinal cord, Rat spinal cord, Mouse sciatic nerve, Neuro-2a and PC-12 lysates. IHC-P: Human colon, Mouse colon and Rat colon tissues. IHC-Fr: Mouse and rat colon tissue. ICC: SH-SY5Y and Neuro-2a cells. Flow:: SH-SY5Y and Neuro-2a cells. IP: Human spinal cord lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR23445-28
Isotype

IgG
Research areas
- Neuroscience
- Development

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab246502 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
Flow Cyt (Intra)		1/50.
WB		1/1000. Detects a band of approximately 58, 56, 45, 28 kDa (predicted molecular weight: 54 kDa).
IP		1/30.
IHC-P	(3)	1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. Use at 1/10,000 dilution for mouse and rat.
IHC-Fr		1/250. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
ICC/IF		1/100.

Notes
Flow Cyt (Intra) 1/50.
WB 1/1000. Detects a band of approximately 58, 56, 45, 28 kDa (predicted molecular weight: 54 kDa).
IP 1/30.
IHC-P 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. Use at 1/10,000 dilution for mouse and rat.
IHC-Fr 1/250. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
ICC/IF 1/100.

Function

Class-III neuronal intermediate filament protein.
Sequence similarities

Belongs to the intermediate filament family.
Target information above from: UniProt accession P41219 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 5630 Human
- Entrez Gene: 19132 Mouse
- Entrez Gene: 24688 Rat
- Omim: 170710 Human
- SwissProt: P41219 Human
- SwissProt: P15331 Mouse
- SwissProt: P21807 Rat
- Unigene: 37044 Human
- Unigene: 2477 Mouse
- Unigene: 11086 Rat
see all
Alternative names
- NEF 4 antibody
- NEF4 antibody
- Neurofilament 4 (57kD) antibody
- Neurofilament 4 antibody
- Perf antibody
- PERI_HUMAN antibody
- Peripherin antibody
- PRPH 1 antibody
- prph antibody
- PRPH1 antibody
see all

Western blot - Anti-Peripherin antibody [EPR23445-28] (ab246502)

All lanes : Anti-Peripherin antibody [EPR23445-28] (ab246502) at 1/1000 dilution

Lane 1 : Mouse dorsal ganglion lysate
Lane 2 : Rat dorsal ganglion lysate
Lane 3 : Mouse spinal cord lysate
Lane 4 : Rat spinal cord lysate
Lane 5 : Mouse sciatic nerve lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 54 kDa
Observed band size: 28,45,56,58 kDa why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: 26 seconds.

The 58KD, 56KD, 45KD and 28KD isoforms of peripherin observed are consistent with what has been described in the literature (PMID: 20587592,18287500,17475883).
Western blot - Anti-Peripherin antibody [EPR23445-28] (ab246502)

All lanes : Anti-Peripherin antibody [EPR23445-28] (ab246502) at 1/1000 dilution

Lane 1 : Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg
Lane 2 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 10 µg

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 54 kDa
Observed band size: 45,56,58 kDa why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: 6 seconds.

The 58KD, 56KD and 45KD isoforms of peripherin observed are consistent with what has been described in the literature (PMID: 20587592, 18287500, 17475883).
Western blot - Anti-Peripherin antibody [EPR23445-28] (ab246502)

All lanes : Anti-Peripherin antibody [EPR23445-28] (ab246502) at 1/1000 dilution

Lane 1 : Human spinal cord lysate
Lane 2 : Human lower limb nerve lysate
Lane 3 : Human nerve lysate
Lane 4 : SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate
Lane 5 : Human kidney lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) or VeriBlot for IP secondary antibody (HRP) (ab131366) at 1/100000 dilution

Predicted band size: 54 kDa
Observed band size: 45,56,58 kDa why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: 37 seconds.

The 58KD, 56KD, and 45KD isoforms of peripherin observed are consistent with what has been described in the literature (PMID: 20587592, 18287500, 17475883).

Negative control: Kidney (PMID: 15132161).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peripherin antibody [EPR23445-28] (ab246502)

Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling Peripherin with ab246502 at 1/10,000 dilution (0.05 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the ganglion cells in rat colon. The section was incubated with ab246502 for 10 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peripherin antibody [EPR23445-28] (ab246502)

Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Peripherin with ab246502 at 1/10,000 dilution (0.05 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the ganglion cells in mouse colon. The section was incubated with ab246502 for 10 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peripherin antibody [EPR23445-28] (ab246502)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Peripherin with ab246502 at 1/2000 dilution (0.227 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the ganglion cells in human colon (PMID: 26469323). The section was incubated with ab246502 for 10 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Immunoprecipitation - Anti-Peripherin antibody [EPR23445-28] (ab246502)

Peripherin was immunoprecipitated from 0.35 mg Human spinal cord lysate with ab246502 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab246502 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1: Human spinal cord lysate 10ug

Lane 2: ab246502 IP in Human spinal cord lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab246502 in Human spinal cord lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 40 seconds.

The 58KD isoform of peripherin observed is consistent with what has been described in the literature (PMID: 20587592, 18287500, 17475883).
Flow Cytometry (Intracellular) - Anti-Peripherin antibody [EPR23445-28] (ab246502)

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized Neuro-2a (mouse neuroblastoma neuroblast) cells labelling Peripherin with ab246502 at 1/50 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Flow Cytometry (Intracellular) - Anti-Peripherin antibody [EPR23445-28] (ab246502)

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized SH-SY5Y (human neuroblastoma epithelial cell) cells labelling Peripherin with ab246502 at 1/50 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Immunohistochemistry (Frozen sections) - Anti-Peripherin antibody [EPR23445-28] (ab246502)

Immunohistochemical analysis of frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized rat colon tissue labeling Peripherin with ab246502 at 1/250 dilution, followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 dilution (Green). Positive staining on the ganglion cells in rat colon is observed. The nuclear counter stain is DAPI (Blue).

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.
Immunohistochemistry (Frozen sections) - Anti-Peripherin antibody [EPR23445-28] (ab246502)

Immunohistochemical analysis of frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized mouse colon tissue labeling Peripherin with ab246502 at 1/250 dilution, followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 dilution (Green). Positive staining on the ganglion cells in mouse colon is observed. The nuclear counter stain is DAPI (Blue).

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Secondary antibody only control: Used PBS instead of primary antibody, secondary is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-Peripherin antibody [EPR23445-28] (ab246502)

Immunofluorescent analysis of4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Neuro-2a (mouse neuroblastoma neuroblast) cells labeling Peripherin with ab246502 at 1/100 dilution, followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in Neuro-2a cell line. Tubulin is detected using ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) at 1/200 dilution (Red). The nuclear counter stain is DAPI (Blue).

Secondary antibody only control: Used PBS instead of primary antibody, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-Peripherin antibody [EPR23445-28] (ab246502)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SH-SY5Y (human neuroblastoma epithelial cell) cells labeling Peripherin with ab246502 at 1/100 dilution, followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in SH-SY5Y cell line. Tubulin is detected using ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) at 1/200 dilution (Red). The nuclear counter stain is DAPI (Blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit at 1/1000 dilution.
Anti-Peripherin antibody [EPR23445-28] (ab246502)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

SDS download

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Datasheet download

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Publishing research using ab246502? Please let us know so that we can cite the reference in this datasheet.

ab246502 has not yet been referenced specifically in any publications.

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1-3 of 3 Abreviews or Q&A

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Rat Tissue sections (Colon)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Citric acid

Permeabilization

Specification

Colon

Blocking step

BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Carl Hobbs

Verified customer

Submitted Mar 14 2020

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (Colon)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Citric acid

Permeabilization

Specification

Colon

Blocking step

BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Carl Hobbs

Verified customer

Submitted Mar 14 2020

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (Lung)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Citric acid

Permeabilization

Specification

Lung

Blocking step

BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Carl Hobbs

Verified customer

Submitted Mar 10 2020

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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

Applications

The Abpromise guarantee

Target

Function

Sequence similarities

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

Certificate of Compliance

References (0)

Customer reviews and Q&As

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Peripherin antibody [EPR23445-28]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Peripherin antibody [EPR23445-28]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Peripherin antibody [EPR23445-28]