Recombinant Anti-Peripherin antibody [EPR23445-28] - BSA and Azide free (ab269861)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23445-28] to Peripherin - BSA and Azide free
- Suitable for: IP, Flow Cyt (Intra), WB, IHC-P, IHC-Fr, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Peripherin antibody [EPR23445-28] - BSA and Azide free
See all Peripherin primary antibodies -
Description
Rabbit monoclonal [EPR23445-28] to Peripherin - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IP, Flow Cyt (Intra), WB, IHC-P, IHC-Fr, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human spinal cord, Human lower limb nerve, Human nerve, SH-SY5Y, Mouse dorsal ganglion, Rat dorsal ganglion, Mouse spinal cord, Rat spinal cord, Mouse sciatic nerve, Neuro-2a and PC-12 lysates. IHC-P: Human colon, Mouse colon and Rat colon tissues. IHC-Fr: Mouse and rat colon tissue. ICC: SH-SY5Y and Neuro-2a cells. Flow:: SH-SY5Y and Neuro-2a cells. IP: Human spinal cord lysate.
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General notes
ab269861 is the carrier-free version of ab246502.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23445-28 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-Peripherin antibody [EPR23445-28] (ab246502)
- Alexa Fluor® 647 Anti-Peripherin antibody [EPR23445-28] (ab275151)
- PE Anti-Peripherin antibody [EPR23445-28] (ab305976)
- APC Anti-Peripherin antibody [EPR23445-28] (ab305977)
- HRP Anti-Peripherin antibody [EPR23445-28] (ab305978)
- Alexa Fluor® 555 Anti-Peripherin antibody [EPR23445-28] (ab311892)
- Alexa Fluor® 568 Anti-Peripherin antibody [EPR23445-28] (ab312363)
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab269861 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at an assay dependent concentration.
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Detects a band of approximately 58, 56, 45, 28 kDa (predicted molecular weight: 54 kDa).
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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IHC-Fr |
Use at an assay dependent concentration.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). |
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ICC/IF |
Use at an assay dependent concentration.
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Notes |
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IP
Use at an assay dependent concentration. |
Flow Cyt (Intra)
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Detects a band of approximately 58, 56, 45, 28 kDa (predicted molecular weight: 54 kDa). |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IHC-Fr
Use at an assay dependent concentration. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). |
ICC/IF
Use at an assay dependent concentration. |
Target
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Function
Class-III neuronal intermediate filament protein. -
Sequence similarities
Belongs to the intermediate filament family. - Information by UniProt
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Database links
- Entrez Gene: 5630 Human
- Entrez Gene: 19132 Mouse
- Entrez Gene: 24688 Rat
- Omim: 170710 Human
- SwissProt: P41219 Human
- SwissProt: P15331 Mouse
- SwissProt: P21807 Rat
- Unigene: 37044 Human
see all -
Alternative names
- NEF 4 antibody
- NEF4 antibody
- Neurofilament 4 (57kD) antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling Peripherin with ab246502 at 1/10,000 dilution (0.05 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the ganglion cells in rat colon is observed. The section was incubated with ab246502 for 10 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246502).
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Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Peripherin with ab246502 at 1/10,000 dilution (0.05 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the ganglion cells in mouse colon is observed. The section was incubated with ab246502 for 10 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246502).
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Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Peripherin with ab246502 at 1/2000 dilution (0.227 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the ganglion cells in human colon (PMID: 26469323). The section was incubated with ab246502 for 10 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246502).
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Peripherin was immunoprecipitated from 0.35 mg Human spinal cord lysate with ab246502 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab246502 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.
Lane 1: Human spinal cord lysate 10ug
Lane 2: ab246502 IP in Human spinal cord lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab246502 in Human spinal cord lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 40 seconds.
The 58KD isoform of peripherin observed is consistent with what has been described in the literature (PMID: 20587592, 18287500, 17475883).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246502).
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Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized Neuro-2a (mouse neuroblastoma neuroblast) cells labelling Peripherin with ab246502 at 1/50 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor®488, ab150077) at 1/2000 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246502).
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Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized SH-SY5Y (human neuroblastoma epithelial cell) cells labelling Peripherin with ab246502 at 1/50 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor®488, ab150077) at 1/2000 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246502).
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Immunohistochemical analysis of frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized rat colon tissue labeling Peripherin with ab246502 at 1/250 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution (Green). Positive staining on the ganglion cells in rat colon is observed. The nuclear counter stain is DAPI (Blue).
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246502).
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Immunohistochemical analysis of frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized mouse colon tissue labeling Peripherin with ab246502 at 1/250 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution (Green). Positive staining on the ganglion cells in mouse colon is observed. The nuclear counter stain is DAPI (Blue).
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246502).
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Neuro-2a (mouse neuroblastoma neuroblast) cells labeling Peripherin with ab246502 at 1/100 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in Neuro-2a cell line. Tubulin is detected using ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 dilution (Red). The nuclear counter stain is DAPI (Blue).
Secondary antibody only control: Used PBS instead of primary antibody, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246502).
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Immunofluorescent analysis of4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SH-SY5Y (human neuroblastoma epithelial cell) cells labeling Peripherin with ab246502 at 1/100 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in SH-SY5Y cell line. Tubulin is detected using ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 dilution (Red). The nuclear counter stain is DAPI (Blue).
Secondary antibody only control: Used PBS instead of primary antibody, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246502).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab269861 has not yet been referenced specifically in any publications.