Recombinant Anti-PERK antibody [EPR19876-294] (ab229912)
![Western blot - Anti-PERK antibody [EPR19876-294] (ab229912)](https://www.abcam.com/ps/products/229/ab229912/Images/ab229912-344446-229912WB2.jpg "Western blot - Anti-PERK antibody [EPR19876-294] (ab229912)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19876-294] to PERK
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-PERK antibody [EPR19876-294]
See all PERK primary antibodies -
Description
Rabbit monoclonal [EPR19876-294] to PERK -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Wild-type HAP1, HeLa, U-87 MG, HEK-293T, MCF7, RAW 264.7, NIH/3T3 and PC-12 whole cell lysates; Mouse brain lysate; Rat brain and liver lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19876-294 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab229912 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (3) |
1/1000. Detects a band of approximately 150 kDa (predicted molecular weight: 125 kDa).
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| Notes |
|---|
|
WB
1/1000. Detects a band of approximately 150 kDa (predicted molecular weight: 125 kDa). |
Application notes
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
Target
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Function
Phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin D1. -
Tissue specificity
Ubiquitous. A high level expression is seen in secretory tissues. -
Involvement in disease
Defects in EIF2AK3 are the cause of Wolcott-Rallison syndrome (WRS) [MIM:226980]; also known as multiple epiphyseal dysplasia with early-onset diabetes mellitus. WRS is a rare autosomal recessive disorder, characterized by permanent neonatal or early infancy insulin-dependent diabetes and, at a later age, epiphyseal dysplasia, osteoporosis, growth retardation and other multisystem manifestations, such as hepatic and renal dysfunctions, mental retardation and cardiovascular abnormalities. -
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.
Contains 1 protein kinase domain. -
Domain
The lumenal domain senses perturbations in protein folding in the ER, probably through reversible interaction with HSPA5/BIP. -
Post-translational
modificationsAutophosphorylated.
N-glycosylated. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 9451 Human
- Entrez Gene: 13666 Mouse
- Entrez Gene: 29702 Rat
- Omim: 604032 Human
- SwissProt: Q9NZJ5 Human
- SwissProt: Q9Z2B5 Mouse
- SwissProt: Q9Z1Z1 Rat
- Unigene: 591589 Human
see all -
Alternative names
- DKFZp781H1925 antibody
- E2AK3_HUMAN antibody
- EC 2.7.11.1 antibody
see all
Images
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Western blot - Anti-PERK antibody [EPR19876-294] (ab229912)All lanes : Anti-PERK antibody [EPR19876-294] (ab229912) at 1/1000 dilution
Lane 1 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 2 : NIH/3T3 (mouse embyro fibroblast cell line) whole cell lysate
Lane 3 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 4 : Mouse brain lysate
Lane 5 : Rat brain lysate
Lane 6 : Rat liver lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 125 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?Exposure time: Lanes 1-2: 37 seconds; Lanes 3-6: 3 minutes.
Blocking/Dilution buffer: 5% NFDM/TBST.
This antibody also recognizes unidentifiable proteins below 37 kDa.
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![Western blot - Anti-PERK antibody [EPR19876-294] (ab229912)](https://www.abcam.com/ps/products/229/ab229912/Images/ab229912-344445-229912WB1.jpg)
Western blot - Anti-PERK antibody [EPR19876-294] (ab229912)All lanes : Anti-PERK antibody [EPR19876-294] (ab229912) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : PERK knockout HAP1 whole cell lysate
Lane 3 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 5 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 6 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 125 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Exposure time: 125 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
ab229912 was shown to specifically react with PERK in wild-type HAP1 cells as signal was lost in PERK knockout cells. Wild-type and PERK knockout samples were subjected to SDS-PAGE. ab229912 and ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.
This antibody also recognizes unidentifiable proteins below 75 kDa.
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![Anti-PERK antibody [EPR19876-294] (ab229912)](https://www.abcam.com/ps/products/229/ab229912/Images/ab229912-3-benefits-of-recombinant-antibodies.png)
Anti-PERK antibody [EPR19876-294] (ab229912)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (10)
ab229912 has been referenced in 10 publications.
- Echavarría-Consuegra L et al. Manipulation of the unfolded protein response: A pharmacological strategy against coronavirus infection. PLoS Pathog 17:e1009644 (2021). PubMed: 34138976
- Rong K et al. Alendronate Alleviated Femoral Head Necrosis and Upregulated BMP2/EIF2AK3/EIF2A/ATF4 Pathway in Liquid Nitrogen Treated Rats. Drug Des Devel Ther 15:1717-1724 (2021). PubMed: 33935494
- Huang X et al. Overexpression of the transcription factors OCT4 and KLF4 improves motor function after spinal cord injury. CNS Neurosci Ther N/A:N/A (2020). PubMed: 32449258
- Wang WM et al. Kupffer Cell-Derived TNF-a Triggers the Apoptosis of Hepatic Stellate Cells through TNF-R1/Caspase 8 due to ER Stress. Biomed Res Int 2020:8035671 (2020). PubMed: 32802876
- Guo J et al. PERK controls bone homeostasis through the regulation of osteoclast differentiation and function. Cell Death Dis 11:847 (2020). PubMed: 33051453
- Wang H et al. MicroRNA-490-3p suppresses the proliferation and invasion of hepatocellular carcinoma cells via targeting TMOD3. Oncol Lett 20:95 (2020). PubMed: 32831914
- Ye J et al. Tau-induced upregulation of C/EBPß-TRPC1-SOCE signaling aggravates tauopathies: A vicious cycle in Alzheimer neurodegeneration. Aging Cell 19:e13209 (2020). PubMed: 32815315
- Li M et al. Endoplasmic reticulum stress regulates cell injury in lipopolysaccharide-induced nerve cells. J Int Med Res 48:300060520949762 (2020). PubMed: 32910707
- Zhang B et al. Mitochondrial dysfunction and endoplasmic reticulum stress in calf hepatocytes are associated with fatty acid-induced ORAI calcium release-activated calcium modulator 1 signaling. J Dairy Sci 103:11945-11956 (2020). PubMed: 32981726
- Doycheva D et al. Adenoviral TMBIM6 vector attenuates ER-stress-induced apoptosis in a neonatal hypoxic-ischemic rat model. Dis Model Mech 12:N/A (2019). PubMed: 31636086