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    products/primary-antibodies/perk-antibody-epr19876-294-ab229912.pdf

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Epigenetics and Nuclear Signaling DNA / RNA Translation Regulation
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-PERK antibody [EPR19876-294] (ab229912)

  • Datasheet
  • SDS
  • Certificate of Compliance
Reviews (5) Submit a question References (10)

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Western blot - Anti-PERK antibody [EPR19876-294] (ab229912)
  • Western blot - Anti-PERK antibody [EPR19876-294] (ab229912)
  • Anti-PERK antibody [EPR19876-294] (ab229912)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19876-294] to PERK
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

You may also be interested in

Protein
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Recombinant human PERK protein (ab101115)
Biochemical
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Staurosporine (DMSO solution), protein kinase inhibitor (ab146588)
Primary
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Anti-eIF2A antibody [EPR11042] (ab169528)

View more associated products

Overview

  • Product name

    Anti-PERK antibody [EPR19876-294]
    See all PERK primary antibodies
  • Description

    Rabbit monoclonal [EPR19876-294] to PERK
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Wild-type HAP1, HeLa, U-87 MG, HEK-293T, MCF7, RAW 264.7, NIH/3T3 and PC-12 whole cell lysates; Mouse brain lysate; Rat brain and liver lysates.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR19876-294
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • Regulation
    • Epigenetics and Nuclear Signaling
    • RNAi
    • Eukaryotic Initiation factors (eIF's)
    • Metabolism
    • Types of disease
    • Diabetes
    • Metabolism
    • Types of disease
    • Obesity
    • Metabolism
    • Types of disease
    • Metabolic disorders

Associated products

  • Alternative Versions

    • Anti-PERK antibody [EPR19876-294] - BSA and Azide free (ab254249)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Recombinant Protein

    • Recombinant human PERK protein (ab101115)
  • Related Products

    • Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab229912 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (3)
1/1000. Detects a band of approximately 150 kDa (predicted molecular weight: 125 kDa).
Notes
WB
1/1000. Detects a band of approximately 150 kDa (predicted molecular weight: 125 kDa).
Application notes
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.

Target

  • Function

    Phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin D1.
  • Tissue specificity

    Ubiquitous. A high level expression is seen in secretory tissues.
  • Involvement in disease

    Defects in EIF2AK3 are the cause of Wolcott-Rallison syndrome (WRS) [MIM:226980]; also known as multiple epiphyseal dysplasia with early-onset diabetes mellitus. WRS is a rare autosomal recessive disorder, characterized by permanent neonatal or early infancy insulin-dependent diabetes and, at a later age, epiphyseal dysplasia, osteoporosis, growth retardation and other multisystem manifestations, such as hepatic and renal dysfunctions, mental retardation and cardiovascular abnormalities.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.
    Contains 1 protein kinase domain.
  • Domain

    The lumenal domain senses perturbations in protein folding in the ER, probably through reversible interaction with HSPA5/BIP.
  • Post-translational
    modifications

    Autophosphorylated.
    N-glycosylated.
  • Cellular localization

    Endoplasmic reticulum membrane.
  • Target information above from: UniProt accession Q9NZJ5 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 9451 Human
    • Entrez Gene: 13666 Mouse
    • Entrez Gene: 29702 Rat
    • Omim: 604032 Human
    • SwissProt: Q9NZJ5 Human
    • SwissProt: Q9Z2B5 Mouse
    • SwissProt: Q9Z1Z1 Rat
    • Unigene: 591589 Human
    • Unigene: 247167 Mouse
    • Unigene: 24897 Rat
    see all
  • Alternative names

    • DKFZp781H1925 antibody
    • E2AK3_HUMAN antibody
    • EC 2.7.11.1 antibody
    • Eif2ak3 antibody
    • Eukaryotic translation initiation factor 2 alpha kinase 3 antibody
    • Eukaryotic translation initiation factor 2-alpha kinase 3 antibody
    • Heme regulated EIF2 alpha kinase antibody
    • HRI antibody
    • HsPEK antibody
    • Pancreatic eIF2 alpha kinase antibody
    • Pancreatic eIF2-alpha kinase antibody
    • PEK antibody
    • PRKR like endoplasmic reticulum kinase antibody
    • PRKR-like endoplasmic reticulum kinase antibody
    • WRS antibody
    see all

Images

  • Western blot - Anti-PERK antibody [EPR19876-294] (ab229912)
    Western blot - Anti-PERK antibody [EPR19876-294] (ab229912)
    All lanes : Anti-PERK antibody [EPR19876-294] (ab229912) at 1/1000 dilution

    Lane 1 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 2 : NIH/3T3 (mouse embyro fibroblast cell line) whole cell lysate
    Lane 3 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 4 : Mouse brain lysate
    Lane 5 : Rat brain lysate
    Lane 6 : Rat liver lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 125 kDa
    Observed band size: 150 kDa why is the actual band size different from the predicted?



    Exposure time: Lanes 1-2: 37 seconds; Lanes 3-6: 3 minutes.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    This antibody also recognizes unidentifiable proteins below 37 kDa.

  • Western blot - Anti-PERK antibody [EPR19876-294] (ab229912)
    Western blot - Anti-PERK antibody [EPR19876-294] (ab229912)
    All lanes : Anti-PERK antibody [EPR19876-294] (ab229912) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : PERK knockout HAP1 whole cell lysate
    Lane 3 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
    Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 5 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 6 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 125 kDa
    Observed band size: 150 kDa why is the actual band size different from the predicted?


    Exposure time: 125 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    ab229912 was shown to specifically react with PERK in wild-type HAP1 cells as signal was lost in PERK knockout cells. Wild-type and PERK knockout samples were subjected to SDS-PAGE. ab229912 and ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.

    This antibody also recognizes unidentifiable proteins below 75 kDa.

  • Anti-PERK antibody [EPR19876-294] (ab229912)
    Anti-PERK antibody [EPR19876-294] (ab229912)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (10)

Publishing research using ab229912? Please let us know so that we can cite the reference in this datasheet.

ab229912 has been referenced in 10 publications.

  • Echavarría-Consuegra L  et al. Manipulation of the unfolded protein response: A pharmacological strategy against coronavirus infection. PLoS Pathog 17:e1009644 (2021). PubMed: 34138976
  • Rong K  et al. Alendronate Alleviated Femoral Head Necrosis and Upregulated BMP2/EIF2AK3/EIF2A/ATF4 Pathway in Liquid Nitrogen Treated Rats. Drug Des Devel Ther 15:1717-1724 (2021). PubMed: 33935494
  • Huang X  et al. Overexpression of the transcription factors OCT4 and KLF4 improves motor function after spinal cord injury. CNS Neurosci Ther N/A:N/A (2020). PubMed: 32449258
  • Wang WM  et al. Kupffer Cell-Derived TNF-a Triggers the Apoptosis of Hepatic Stellate Cells through TNF-R1/Caspase 8 due to ER Stress. Biomed Res Int 2020:8035671 (2020). PubMed: 32802876
  • Guo J  et al. PERK controls bone homeostasis through the regulation of osteoclast differentiation and function. Cell Death Dis 11:847 (2020). PubMed: 33051453
  • Wang H  et al. MicroRNA-490-3p suppresses the proliferation and invasion of hepatocellular carcinoma cells via targeting TMOD3. Oncol Lett 20:95 (2020). PubMed: 32831914
  • Ye J  et al. Tau-induced upregulation of C/EBPß-TRPC1-SOCE signaling aggravates tauopathies: A vicious cycle in Alzheimer neurodegeneration. Aging Cell 19:e13209 (2020). PubMed: 32815315
  • Li M  et al. Endoplasmic reticulum stress regulates cell injury in lipopolysaccharide-induced nerve cells. J Int Med Res 48:300060520949762 (2020). PubMed: 32910707
  • Zhang B  et al. Mitochondrial dysfunction and endoplasmic reticulum stress in calf hepatocytes are associated with fatty acid-induced ORAI calcium release-activated calcium modulator 1 signaling. J Dairy Sci 103:11945-11956 (2020). PubMed: 32981726
  • Doycheva D  et al. Adenoviral TMBIM6 vector attenuates ER-stress-induced apoptosis in a neonatal hypoxic-ischemic rat model. Dis Model Mech 12:N/A (2019). PubMed: 31636086

Customer reviews and Q&As

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1-5 of 5 Abreviews or Q&A

Western blot abreview for Anti-PERK antibody [EPR19876-294]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Cell lysate - whole cell (Rat hepatocytes)
Gel Running Conditions
Reduced Denaturing (8% SDS-PAGE)
Loading amount
35 µg
Specification
Rat hepatocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Dec 10 2021

Western blot abreview for Anti-PERK antibody [EPR19876-294]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (PC-3 cells - human prostate cancer cells)
Gel Running Conditions
Reduced Denaturing (8% SDS-PAGE)
Loading amount
40 µg
Specification
PC-3 cells - human prostate cancer cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Nov 29 2021

Immunocytochemistry/ Immunofluorescence abreview for Anti-PERK antibody [EPR19876-294]

Excellent
Abreviews
Abreviews
This product is known to not work in this application or species.
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Rat hepatocytes)
Permeabilization
Yes - 0.2% Triton X-100 in PBS
Specification
Rat hepatocytes
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Armen Petrosyan

Verified customer

Submitted Nov 25 2021

Immunocytochemistry/ Immunofluorescence abreview for Anti-PERK antibody [EPR19876-294]

Excellent
Abreviews
Abreviews
This product is known to not work in this application or species.
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Mice hepatocytes)
Permeabilization
Yes - 0.2% Triton X-100 in PBS
Specification
Mice hepatocytes
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Armen Petrosyan

Verified customer

Submitted Oct 12 2021

Western blot abreview for Anti-PERK antibody [EPR19876-294]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mice hepatocytes)
Gel Running Conditions
Reduced Denaturing (8% SDS-PAGE)
Loading amount
40 µg
Specification
Mice hepatocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Armen Petrosyan

Verified customer

Submitted Aug 05 2021

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