Recombinant Anti-PERK antibody [EPR19876-294] (ab229912)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19876-294] to PERK
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-PERK antibody [EPR19876-294]
See all PERK primary antibodies -
Description
Rabbit monoclonal [EPR19876-294] to PERK -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Wild-type HAP1, HeLa, U-87 MG, HEK-293T, MCF7, RAW 264.7, NIH/3T3 and PC-12 whole cell lysates; Mouse brain lysate; Rat brain and liver lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19876-294 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab229912 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (3) |
1/1000. Detects a band of approximately 150 kDa (predicted molecular weight: 125 kDa).
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Notes |
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WB
1/1000. Detects a band of approximately 150 kDa (predicted molecular weight: 125 kDa). |
Target
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Function
Phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin D1. -
Tissue specificity
Ubiquitous. A high level expression is seen in secretory tissues. -
Involvement in disease
Defects in EIF2AK3 are the cause of Wolcott-Rallison syndrome (WRS) [MIM:226980]; also known as multiple epiphyseal dysplasia with early-onset diabetes mellitus. WRS is a rare autosomal recessive disorder, characterized by permanent neonatal or early infancy insulin-dependent diabetes and, at a later age, epiphyseal dysplasia, osteoporosis, growth retardation and other multisystem manifestations, such as hepatic and renal dysfunctions, mental retardation and cardiovascular abnormalities. -
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.
Contains 1 protein kinase domain. -
Domain
The lumenal domain senses perturbations in protein folding in the ER, probably through reversible interaction with HSPA5/BIP. -
Post-translational
modificationsAutophosphorylated.
N-glycosylated. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 9451 Human
- Entrez Gene: 13666 Mouse
- Entrez Gene: 29702 Rat
- Omim: 604032 Human
- SwissProt: Q9NZJ5 Human
- SwissProt: Q9Z2B5 Mouse
- SwissProt: Q9Z1Z1 Rat
- Unigene: 591589 Human
see all -
Alternative names
- DKFZp781H1925 antibody
- E2AK3_HUMAN antibody
- EC 2.7.11.1 antibody
see all
Images
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All lanes : Anti-PERK antibody [EPR19876-294] (ab229912) at 1/1000 dilution
Lane 1 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 2 : NIH/3T3 (mouse embyro fibroblast cell line) whole cell lysate
Lane 3 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 4 : Mouse brain lysate
Lane 5 : Rat brain lysate
Lane 6 : Rat liver lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 125 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?Exposure time: Lanes 1-2: 37 seconds; Lanes 3-6: 3 minutes.
Blocking/Dilution buffer: 5% NFDM/TBST.
This antibody also recognizes unidentifiable proteins below 37 kDa.
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All lanes : Anti-PERK antibody [EPR19876-294] (ab229912) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : PERK knockout HAP1 whole cell lysate
Lane 3 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 5 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 6 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 125 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Exposure time: 125 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
ab229912 was shown to specifically react with PERK in wild-type HAP1 cells as signal was lost in PERK knockout cells. Wild-type and PERK knockout samples were subjected to SDS-PAGE. ab229912 and ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.
This antibody also recognizes unidentifiable proteins below 75 kDa.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (10)
ab229912 has been referenced in 10 publications.
- Echavarría-Consuegra L et al. Manipulation of the unfolded protein response: A pharmacological strategy against coronavirus infection. PLoS Pathog 17:e1009644 (2021). PubMed: 34138976
- Rong K et al. Alendronate Alleviated Femoral Head Necrosis and Upregulated BMP2/EIF2AK3/EIF2A/ATF4 Pathway in Liquid Nitrogen Treated Rats. Drug Des Devel Ther 15:1717-1724 (2021). PubMed: 33935494
- Huang X et al. Overexpression of the transcription factors OCT4 and KLF4 improves motor function after spinal cord injury. CNS Neurosci Ther N/A:N/A (2020). PubMed: 32449258
- Wang WM et al. Kupffer Cell-Derived TNF-a Triggers the Apoptosis of Hepatic Stellate Cells through TNF-R1/Caspase 8 due to ER Stress. Biomed Res Int 2020:8035671 (2020). PubMed: 32802876
- Guo J et al. PERK controls bone homeostasis through the regulation of osteoclast differentiation and function. Cell Death Dis 11:847 (2020). PubMed: 33051453
- Wang H et al. MicroRNA-490-3p suppresses the proliferation and invasion of hepatocellular carcinoma cells via targeting TMOD3. Oncol Lett 20:95 (2020). PubMed: 32831914
- Ye J et al. Tau-induced upregulation of C/EBPß-TRPC1-SOCE signaling aggravates tauopathies: A vicious cycle in Alzheimer neurodegeneration. Aging Cell 19:e13209 (2020). PubMed: 32815315
- Li M et al. Endoplasmic reticulum stress regulates cell injury in lipopolysaccharide-induced nerve cells. J Int Med Res 48:300060520949762 (2020). PubMed: 32910707
- Zhang B et al. Mitochondrial dysfunction and endoplasmic reticulum stress in calf hepatocytes are associated with fatty acid-induced ORAI calcium release-activated calcium modulator 1 signaling. J Dairy Sci 103:11945-11956 (2020). PubMed: 32981726
- Doycheva D et al. Adenoviral TMBIM6 vector attenuates ER-stress-induced apoptosis in a neonatal hypoxic-ischemic rat model. Dis Model Mech 12:N/A (2019). PubMed: 31636086