For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    products/primary-antibodies/peroxiredoxin-2prp-antibody-epr5154-ab109367.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Neuroscience Neurology process Neurodegenerative disease Alzheimer's disease Other
Share by email
Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (ab109367)

  • Datasheet
  • SDS
Reviews (2) Submit a question References (35)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (ab109367)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (ab109367)
  • Immunocytochemistry/ Immunofluorescence - Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (ab109367)
  • Western blot - Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (ab109367)
  • Western blot - Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (ab109367)
  • Western blot - Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (ab109367)
  • Flow Cytometry (Intracellular) - Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (ab109367)
  • Flow Cytometry (Intracellular) - Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (ab109367)
  • Western blot - Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (ab109367)
  • Immunocytochemistry/ Immunofluorescence - Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (ab109367)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (ab109367)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (ab109367)
  • Immunocytochemistry/ Immunofluorescence - Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (ab109367)
  • Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (ab109367)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5154] to Peroxiredoxin 2/PRP
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free HRP

You may also be interested in

Primary
Product image
Anti-Peroxiredoxin 1/PAG antibody [EPR5433] (ab109498)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Knockout
Product image
Human PRDX2 (Peroxiredoxin 2/PRP) knockout HEK-293T cell line (ab266392)

View more associated products

Overview

  • Product name

    Anti-Peroxiredoxin 2/PRP antibody [EPR5154]
    See all Peroxiredoxin 2/PRP primary antibodies
  • Description

    Rabbit monoclonal [EPR5154] to Peroxiredoxin 2/PRP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Pig
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HAP1, HEK-293T, HeLa, LnCaP, and SH-SY5Y cell lysates; Mouse and rat brain tissues. IHC-P: Human endometrial carcinoma, prostatic hyperplasia and stomach tissues. ICC: HeLa cells. Flow Cyt (intra): HeLa cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR5154
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Other
    • Cell Biology
    • Other Antibodies
    • Oxidative Stress
    • Cancer
    • Cancer Metabolism
    • Cellular metabolic process
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Oxidative stress
    • Metabolism
    • Types of disease
    • Neurodegenerative disease
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Alternative Versions

    • Alexa Fluor® 647 Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (ab197041)
    • HRP Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (ab197042)
    • Alexa Fluor® 488 Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (ab197536)
    • Anti-Peroxiredoxin 2/PRP antibody [EPR5154] - BSA and Azide free (ab227988)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human PRDX2 (Peroxiredoxin 2/PRP) knockout HEK-293T cell line (ab266392)
  • KO cell lysates

    • Human PRDX2 (Peroxiredoxin 2/PRP) knockout HEK-293T cell lysate (ab257041)
  • Recombinant Protein

    • Recombinant human Peroxiredoxin 2/PRP protein (ab85331)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab109367 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/200 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.  

WB (1)
1/1000 - 1/10000. Predicted molecular weight: 22 kDa.
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF (1)
1/100 - 1/500.
Notes
Flow Cyt (Intra)
1/200 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.  

WB
1/1000 - 1/10000. Predicted molecular weight: 22 kDa.
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/500.

Target

  • Function

    Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system. It is not able to receive electrons from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2).
  • Sequence similarities

    Belongs to the ahpC/TSA family.
    Contains 1 thioredoxin domain.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession P32119 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7001 Human
    • Entrez Gene: 21672 Mouse
    • Entrez Gene: 29338 Rat
    • Omim: 600538 Human
    • SwissProt: P32119 Human
    • SwissProt: Q61171 Mouse
    • SwissProt: P35704 Rat
    • Unigene: 432121 Human
    • Unigene: 347009 Mouse
    • Unigene: 393373 Mouse
    • Unigene: 2511 Rat
    see all
  • Alternative names

    • Epididymis secretory sperm binding protein Li 2a antibody
    • HEL S 2a antibody
    • MGC4104 antibody
    • Natural killer cell enhancing factor B antibody
    • Natural killer cell-enhancing factor B antibody
    • Natural Killer Enhancing Factor B antibody
    • NKEF B antibody
    • NKEF-B antibody
    • NKEFB antibody
    • Peroxiredoxin 2 antibody
    • Peroxiredoxin-2 antibody
    • PRDX 2 antibody
    • PRDX2 antibody
    • PRDX2_HUMAN antibody
    • PrP antibody
    • PRX2 antibody
    • PRXII antibody
    • PTX1 antibody
    • TDPX1 antibody
    • Thiol Specific Antioxidant 1 antibody
    • Thiol specific antioxidant protein antibody
    • Thiol-specific antioxidant protein antibody
    • Thioredoxin Dependent Peroxide Reductase 1 antibody
    • Thioredoxin peroxidase 1 antibody
    • Thioredoxin-dependent peroxide reductase 1 antibody
    • Torin antibody
    • TPX1 antibody
    • TSA antibody
    see all

Images

  • Western blot - Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (ab109367)
    Western blot - Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (ab109367)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Peroxiredoxin 2/PRP knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: LnCaP cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab109367 observed at 24 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab109367 was shown to specifically react with Peroxiredoxin 2/PRP when Peroxiredoxin 2/PRP knockout samples were used. Wild-type and Peroxiredoxin 2 knockout samples were subjected to SDS-PAGE. ab109367 and ab8245 (loading control to GAPDH) were both diluted 1/1000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (ab109367)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (ab109367)
    Immunohistochemical staining of paraffin embedded human endometrial carcinoma with purified ab109367 at a working dilution of 1/500. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Immunocytochemistry/ Immunofluorescence - Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (ab109367)
    Immunocytochemistry/ Immunofluorescence - Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (ab109367)

    Immunofluorescence staining of HeLa cells with purified ab109367 at a working dilution of 1/100, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 100% methanol and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab109367 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

  • Western blot - Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (ab109367)
    Western blot - Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (ab109367)
    All lanes : Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (ab109367) at 1/1000 dilution

    Lane 1 : Wild-type HEK-293T cell lysate
    Lane 2 : PRDX2 knockout HEK-293T cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 22 kDa
    Observed band size: 22 kDa



    Lanes 1- 2: Merged signal (red and green). Green - ab109367 observed at 22 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

     ab109367 was shown to react with Peroxiredoxin 2/PRP in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266392 (knockout cell lysate ab257041) was used. Wild-type HEK-293T and PRDX2 knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab109367 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (ab109367)
    Western blot - Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (ab109367)
    All lanes : Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (ab109367) at 1/10000 dilution (purified)

    Lane 1 : Mouse brain lysate
    Lane 2 : Rat brain lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 22 kDa
    Observed band size: 22 kDa



    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (ab109367)
    Western blot - Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (ab109367)
    All lanes : Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (ab109367) at 1/10000 dilution (purified)

    Lane 1 : HEK293 whole cell lysate
    Lane 2 : LNCaP whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 22 kDa
    Observed band size: 22 kDa



    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Flow Cytometry (Intracellular) - Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (ab109367)
    Flow Cytometry (Intracellular) - Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (ab109367)

    Overlay histogram showing HeLa cells fixed in 80% methanol and stained with purified ab109367 at a dilution of 1/200 (red line). The secondary antibody used was Alexa Fluorr® 488 goat anti-rabbit at a dilution of 1/500. Rabbit monoclonal IgG was used as an isotype control (black line) and cells incubated in the absence of both primary and secondary antibody were used as a negative control (blue line). 

     

     

  • Flow Cytometry (Intracellular) - Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (ab109367)
    Flow Cytometry (Intracellular) - Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (ab109367)

    Overlay histogram showing HeLa cells stained with unpurifiedab109367 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109367, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

  • Western blot - Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (ab109367)
    Western blot - Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (ab109367)
    All lanes : Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (ab109367) at 1/1000 dilution (Unpurified)

    Lane 1 : 293T cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : LnCaP cell lysate
    Lane 4 : SH-SYSY cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled Goat anti-Rabbit at 1/2000 dilution

    Predicted band size: 22 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (ab109367)
    Immunocytochemistry/ Immunofluorescence - Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (ab109367)

    Immunofluorescent staining of Peroxiredoxin 2/PRP in HeLa cells using unpurified ab109367 at 1/250 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (ab109367)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (ab109367)

    Immunohistochemical analysis of Peroxiredoxin 2/PRP in paraffin-embedded Human prostatic hyperplasia tissue using unpurified ab109367 at 1/1000 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (ab109367)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (ab109367)

    Immunohistochemical analysis of Peroxiredoxin 2/PRP in paraffin-embedded Human stomach tissue using unpurified ab109367 at 1/1000 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (ab109367)
    Immunocytochemistry/ Immunofluorescence - Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (ab109367)Image courtesy of an Abreview submitted by Dr. Kirk McManus, Univ. of Manitoba/Cancer Care MICB, Canada

    Unpurified ab109367 (1/500) staining Peroxiredoxin 2/PRP in asynchronous HeLa cells (green). Cells were fixed in methanol and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.

    See Abreview

  • Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (ab109367)
    Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (ab109367)

Protocols

  • Flow cytometry protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (35)

Publishing research using ab109367? Please let us know so that we can cite the reference in this datasheet.

ab109367 has been referenced in 35 publications.

  • Goncalves RLS  et al. Cardiolipin deficiency in Barth syndrome is not associated with increased superoxide/H2 O2 production in heart and skeletal muscle mitochondria. FEBS Lett 595:415-432 (2021). PubMed: 33112430
  • Russo GL  et al. CRISPR-Mediated Induction of Neuron-Enriched Mitochondrial Proteins Boosts Direct Glia-to-Neuron Conversion. Cell Stem Cell 28:524-534.e7 (2021). PubMed: 33202244
  • Sadvakassova G  et al. Active hematopoiesis triggers exosomal release of PRDX2 that promotes osteoclast formation. Physiol Rep 9:e14745 (2021). PubMed: 33587325
  • Yin GQ  et al. Differential proteomic analysis of children infected with respiratory syncytial virus. Braz J Med Biol Res 54:e9850 (2021). PubMed: 33656056
  • Trstenjak Prebanda M  et al. Altered Expression of Peroxiredoxins in Mouse Model of Progressive Myoclonus Epilepsy upon LPS-Induced Neuroinflammation. Antioxidants (Basel) 10:N/A (2021). PubMed: 33673502
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-2 of 2 Abreviews or Q&A

Western blot abreview for Anti-Peroxiredoxin 2/PRP antibody [EPR5154]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Pig Tissue lysate - other (Muscle sarcoplasmic extract)
Gel Running Conditions
Reduced Denaturing (12.5% acrylamide)
Loading amount
62.5 µg
Specification
Muscle sarcoplasmic extract
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. Shannon Cruzen

Verified customer

Submitted Feb 05 2014

Immunocytochemistry/ Immunofluorescence abreview for Anti-Peroxiredoxin 2 antibody [EPR5154]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Methanol
Permeabilization
No
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Kirk Mcmanus

Verified customer

Submitted Sep 03 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Get resources and offers direct to your inbox Sign up
A-Z by research area
  • Cancer
  • Cardiovascular
  • Cell biology
  • Developmental biology
  • Epigenetics & Nuclear signaling
  • Immunology
  • Metabolism
  • Microbiology
  • Neuroscience
  • Signal transduction
  • Stem cells
A-Z by product type
  • Primary antibodies
  • Secondary antibodies
  • Biochemicals
  • Isotype controls
  • Flow cytometry multi-color selector
  • Kits
  • Loading controls
  • Lysates
  • Peptides
  • Proteins
  • Slides
  • Tags and cell markers
  • Tools & Reagents
Help & support
  • Support
  • Make an Inquiry
  • Protocols & troubleshooting
  • Placing an order
  • RabMAb products
  • Biochemical product FAQs
  • Training
  • Browse by Target
Company
  • Corporate site
  • Investor relations
  • Company news
  • Careers
  • About us
  • Blog
Events
  • Tradeshows
  • Conferences
International websites
  • abcam.cn
  • abcam.co.jp

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
  • Modern slavery statement
© 1998-2023 Abcam plc. All rights reserved.