Recombinant Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (ab128953)
![Western blot - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (ab128953)](https://www.abcam.com/ps/products/128/ab128953/Images/ab128953-263347-anti-peroxiredoxin-3-antibody-epr8115-western-blot.jpg "Western blot - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (ab128953)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8115] to Peroxiredoxin 3/PRDX3
- Suitable for: Flow Cyt (Intra), WB, IP, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115]
See all Peroxiredoxin 3/PRDX3 primary antibodies -
Description
Rabbit monoclonal [EPR8115] to Peroxiredoxin 3/PRDX3 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, 293T, MCF7 and A431 cell lysates. IHC-P: Human endometrium adenocarcinoma and Human liver tissues. Flow Cyt (intra): MCF7 cells IP: HeLa.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.02 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8115 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab128953 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/1000.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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| WB | (2) |
1/10000 - 1/50000. Detects a band of approximately 26 kDa (predicted molecular weight: 28 kDa).
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| IP |
1/100 - 1/500.
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| IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| Notes |
|---|
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Flow Cyt (Intra)
1/1000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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WB
1/10000 - 1/50000. Detects a band of approximately 26 kDa (predicted molecular weight: 28 kDa). |
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IP
1/100 - 1/500. |
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IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Involved in redox regulation of the cell. Protects radical-sensitive enzymes from oxidative damage by a radical-generating system. Acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol. -
Sequence similarities
Belongs to the ahpC/TSA family.
Contains 1 thioredoxin domain. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 10935 Human
- Omim: 604769 Human
- SwissProt: P30048 Human
- Unigene: 523302 Human
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Alternative names
- Antioxidant protein 1 antibody
- AOP 1 antibody
- AOP-1 antibody
see all
Images
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Western blot - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (ab128953)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Peroxiredoxin 3/PRDX3 knockout HAP1 cell lysate (20 µg)
Lane 3: MCF7 cell lysate (20 µg)
Lane 4: A431 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab128953 observed at 24 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab128953 was shown to specifically react with Peroxiredoxin 3/PRDX3 in wild-type HAP1 cells. No band was observed when Peroxiredoxin 3/PRDX3 knockout samples were examined. Wild-type and Peroxiredoxin 3/PRDX3 knockout samples were subjected to SDS-PAGE. ab128953 and ab8245 (loading control to GAPDH) were both diluted 1/10,000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (ab128953)](https://www.abcam.com/ps/products/128/ab128953/Images/ab128953-142844-anti-peroxiredoxin-3-antibody-epr8115-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (ab128953)ab128953, at a 1/250 dilution, staining Peroxiredoxin 3/PRDX3 in paraffin embedded Human endometrium adenocarcinoma tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Flow Cytometry (Intracellular) - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (ab128953)](https://www.abcam.com/ps/products/128/ab128953/Images/ab128953-8-anti-peroxiredoxin-3-prdx3-antibody-epr8115-flowcytometry-mcf7-human.jpg)
Flow Cytometry (Intracellular) - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (ab128953)Flow Cytometry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling Peroxiredoxin 3/PRDX3 with purified ab128953 at 1/1000 dilution (1 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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![Western blot - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (ab128953)](https://www.abcam.com/ps/products/128/ab128953/Images/ab128953-142845-anti-peroxiredoxin-3-antibody-epr8115-western-blot.jpg)
Western blot - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (ab128953)All lanes : Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (ab128953) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 28 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (ab128953)](https://www.abcam.com/ps/products/128/ab128953/Images/ab128953-142843-anti-peroxiredoxin-3-antibody-epr8115-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (ab128953)ab128953, at a 1/250 dilution, staining Peroxiredoxin 3/PRDX3 in paraffin embedded Human liver tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunoprecipitation - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (ab128953)](https://www.abcam.com/ps/products/128/ab128953/Images/ab128953-9-anti-peroxiredoxin-3-prdx3-antibody-epr8115-immunoprecipitation-hela-human.jpg)
Immunoprecipitation - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (ab128953)Peroxiredoxin 3/PRDX3 was immunoprecipitated from 0.35 mg HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10 µg with 128953 at 1/50 dilution (2µg). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10 µg
Lane 2: ab128953 IP in HeLa whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab128953 in HeLa whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Upper bands could be ubiquitinated forms (PMID:21795677)
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![OI-RD Scanning - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (ab128953)](https://www.abcam.com/ps/products/128/ab128953/Images/ab128953-142842-anti-peroxiredoxin-3-antibody-epr8115.jpg)
OI-RD Scanning - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (ab128953) -
![Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (ab128953)](https://www.abcam.com/ps/products/128/ab128953/Images/ab128953-6-benefits-of-recombinant-antibodies.png)
Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (ab128953)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab128953 has been referenced in 7 publications.
- Lovatt M et al. Peroxiredoxin-1 regulates lipid peroxidation in corneal endothelial cells. Redox Biol 30:101417 (2020). PubMed: 31901729
- Su Z et al. Analysis and Identification of Tumorigenic Targets of MicroRNA in Cancer Cells by Photoreactive Chemical Probes. Int J Mol Sci 21:N/A (2020). PubMed: 32102467
- Xu Y et al. Ganoderic Acid D Protects Human Amniotic Mesenchymal Stem Cells against Oxidative Stress-Induced Senescence through the PERK/NRF2 Signaling Pathway. Oxid Med Cell Longev 2020:8291413 (2020). PubMed: 32774686
- Gumucio JP et al. Reduced mitochondrial lipid oxidation leads to fat accumulation in myosteatosis. FASEB J 33:7863-7881 (2019). PubMed: 30939247
- Pan C et al. Inositol-triphosphate 3-kinase B confers cisplatin resistance by regulating NOX4-dependent redox balance. J Clin Invest 129:2431-2445 (2019). PubMed: 31081803
- Touat M et al. DNA repair deficiency sensitizes lung cancer cells to NAD+ biosynthesis blockade. J Clin Invest 128:1671-1687 (2018). PubMed: 29447131
- Shi XJ et al. Pro-Apoptotic Effects of JDA-202, a Novel Natural Diterpenoid, on Esophageal Cancer Through Targeting Peroxiredoxin I. Antioxid Redox Signal 27:73-92 (2017). Human . PubMed: 27650197