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    products/primary-antibodies/peroxiredoxin-3prdx3-antibody-epr8115-ab128953.pdf

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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (ab128953)

  • Datasheet
  • SDS
Reviews (2) Submit a question References (7)

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Western blot - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (ab128953)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (ab128953)
  • Flow Cytometry (Intracellular) - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (ab128953)
  • Western blot - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (ab128953)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (ab128953)
  • Immunoprecipitation - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (ab128953)
  • OI-RD Scanning - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (ab128953)
  • Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (ab128953)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8115] to Peroxiredoxin 3/PRDX3
  • Suitable for: Flow Cyt (Intra), WB, IP, IHC-P
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Overview

  • Product name

    Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115]
    See all Peroxiredoxin 3/PRDX3 primary antibodies
  • Description

    Rabbit monoclonal [EPR8115] to Peroxiredoxin 3/PRDX3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, 293T, MCF7 and A431 cell lysates. IHC-P: Human endometrium adenocarcinoma and Human liver tissues. Flow Cyt (intra): MCF7 cells IP: HeLa.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 1.02 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR8115
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Other
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • NFkB Pathway
    • Cardiovascular
    • Atherosclerosis
    • Ischemia / Reperfusion
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Inflammatory mediators
    • Cancer
    • Cancer Metabolism
    • Cellular metabolic process
    • Metabolism
    • Types of disease
    • Neurodegenerative disease
    • Metabolism
    • Types of disease
    • Cancer
    • Metabolism
    • Types of disease
    • Heart disease

Associated products

  • Alternative Versions

    • Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] - BSA and Azide free (ab234918)
  • Compatible Secondaries

    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
    • A-431 whole cell lysate (ab30132)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab128953 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

WB (2)
1/10000 - 1/50000. Detects a band of approximately 26 kDa (predicted molecular weight: 28 kDa).
IP
1/100 - 1/500.
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Notes
Flow Cyt (Intra)
1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

WB
1/10000 - 1/50000. Detects a band of approximately 26 kDa (predicted molecular weight: 28 kDa).
IP
1/100 - 1/500.
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function

    Involved in redox regulation of the cell. Protects radical-sensitive enzymes from oxidative damage by a radical-generating system. Acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol.
  • Sequence similarities

    Belongs to the ahpC/TSA family.
    Contains 1 thioredoxin domain.
  • Cellular localization

    Mitochondrion.
  • Target information above from: UniProt accession P30048 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 10935 Human
    • Omim: 604769 Human
    • SwissProt: P30048 Human
    • Unigene: 523302 Human
    • Alternative names

      • Antioxidant protein 1 antibody
      • AOP 1 antibody
      • AOP-1 antibody
      • AOP1 antibody
      • HBC189 antibody
      • MER5 antibody
      • MGC104387 antibody
      • MGC24293 antibody
      • mitochondrial antibody
      • peroxiredoxin 3 antibody
      • Peroxiredoxin III antibody
      • Peroxiredoxin-3 antibody
      • PRDX3 antibody
      • PRDX3_HUMAN antibody
      • PRO1748 antibody
      • Protein MER5 homolog antibody
      • PRX III antibody
      • Prx-III antibody
      • PRX3 antibody
      • SP 22 antibody
      • SP-22 antibody
      • SP22 antibody
      • Thioredoxin dependent peroxide reductase mitochondrial antibody
      • Thioredoxin-dependent peroxide reductase antibody
      see all

    Images

    • Western blot - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (ab128953)
      Western blot - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (ab128953)

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: Peroxiredoxin 3/PRDX3 knockout HAP1 cell lysate (20 µg)
      Lane 3: MCF7 cell lysate (20 µg)
      Lane 4: A431 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab128953 observed at 24 kDa. Red - loading control, ab8245, observed at 37 kDa.


      ab128953 was shown to specifically react with Peroxiredoxin 3/PRDX3 in wild-type HAP1 cells. No band was observed when Peroxiredoxin 3/PRDX3 knockout samples were examined. Wild-type and Peroxiredoxin 3/PRDX3 knockout samples were subjected to SDS-PAGE. ab128953 and ab8245 (loading control to GAPDH) were both diluted 1/10,000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (ab128953)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (ab128953)

      ab128953, at a 1/250 dilution, staining Peroxiredoxin 3/PRDX3 in paraffin embedded Human endometrium adenocarcinoma tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Flow Cytometry (Intracellular) - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (ab128953)
      Flow Cytometry (Intracellular) - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (ab128953)

      Flow Cytometry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling Peroxiredoxin 3/PRDX3 with purified ab128953 at 1/1000 dilution (1 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    • Western blot - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (ab128953)
      Western blot - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (ab128953)
      All lanes : Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (ab128953) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T (Human embryonic kidney epithelial cell) cell lysate
      Lane 3 : MCF7 cell lysate
      Lane 4 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 28 kDa
      Observed band size: 26 kDa why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (ab128953)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (ab128953)

      ab128953, at a 1/250 dilution, staining Peroxiredoxin 3/PRDX3 in paraffin embedded Human liver tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunoprecipitation - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (ab128953)
      Immunoprecipitation - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (ab128953)

      Peroxiredoxin 3/PRDX3 was immunoprecipitated from 0.35 mg HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10 µg with 128953 at 1/50 dilution (2µg). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10 µg

      Lane 2: ab128953 IP in HeLa whole cell lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab128953 in HeLa whole cell lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Upper bands could be ubiquitinated forms (PMID:21795677)

    • OI-RD Scanning - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (ab128953)
      OI-RD Scanning - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (ab128953)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (ab128953)
      Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (ab128953)

    Protocols

    • Flow cytometry protocols
    • Immunoprecipitation protocols
    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (7)

    Publishing research using ab128953? Please let us know so that we can cite the reference in this datasheet.

    ab128953 has been referenced in 7 publications.

    • Lovatt M  et al. Peroxiredoxin-1 regulates lipid peroxidation in corneal endothelial cells. Redox Biol 30:101417 (2020). PubMed: 31901729
    • Su Z  et al. Analysis and Identification of Tumorigenic Targets of MicroRNA in Cancer Cells by Photoreactive Chemical Probes. Int J Mol Sci 21:N/A (2020). PubMed: 32102467
    • Xu Y  et al. Ganoderic Acid D Protects Human Amniotic Mesenchymal Stem Cells against Oxidative Stress-Induced Senescence through the PERK/NRF2 Signaling Pathway. Oxid Med Cell Longev 2020:8291413 (2020). PubMed: 32774686
    • Gumucio JP  et al. Reduced mitochondrial lipid oxidation leads to fat accumulation in myosteatosis. FASEB J 33:7863-7881 (2019). PubMed: 30939247
    • Pan C  et al. Inositol-triphosphate 3-kinase B confers cisplatin resistance by regulating NOX4-dependent redox balance. J Clin Invest 129:2431-2445 (2019). PubMed: 31081803
    • Touat M  et al. DNA repair deficiency sensitizes lung cancer cells to NAD+ biosynthesis blockade. J Clin Invest 128:1671-1687 (2018). PubMed: 29447131
    • Shi XJ  et al. Pro-Apoptotic Effects of JDA-202, a Novel Natural Diterpenoid, on Esophageal Cancer Through Targeting Peroxiredoxin I. Antioxid Redox Signal 27:73-92 (2017). Human . PubMed: 27650197

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115]

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Brain)
    Gel Running Conditions
    Reduced Denaturing (12)
    Loading amount
    50 µg
    Specification
    Brain
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jul 12 2016

    Western blot abreview for Anti-Peroxiredoxin 3 antibody [EPR8115]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Tissue lysate - whole (Brain tissue)
    Gel Running Conditions
    Reduced Denaturing (12)
    Loading amount
    50 µg
    Specification
    Brain tissue
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted May 13 2016

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