Recombinant Anti-Peroxiredoxin 5 antibody [EPR14528(B)] (ab180123)
![Western blot - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] (ab180123)](https://www.abcam.com/ps/products/180/ab180123/Images/ab180123-270987-anti-peroxiredoxin-5-antibody-epr14528b-western-blot.jpg "Western blot - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] (ab180123)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14528(B)] to Peroxiredoxin 5
- Suitable for: Flow Cyt (Intra), WB, ICC/IF, IP
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Peroxiredoxin 5 antibody [EPR14528(B)]
See all Peroxiredoxin 5 primary antibodies -
Description
Rabbit monoclonal [EPR14528(B)] to Peroxiredoxin 5 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, ICC/IF, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide within Human Peroxiredoxin 5 aa 50-150. The exact sequence is proprietary.
Database link: P30044 -
Positive control
- HepG2, HeLa, Jurkat and A549 cell lysates; HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR14528(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab180123 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/10.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB |
1/10000 - 1/20000. Detects a band of approximately 17 kDa (predicted molecular weight: 22 kDa).
|
|
| ICC/IF |
1/250.
|
|
| IP |
1/60.
|
| Notes |
|---|
|
Flow Cyt (Intra)
1/10. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/10000 - 1/20000. Detects a band of approximately 17 kDa (predicted molecular weight: 22 kDa). |
|
ICC/IF
1/250. |
|
IP
1/60. |
Target
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Function
Reduces hydrogen peroxide and alkyl hydroperoxides with reducing equivalents provided through the thioredoxin system. Involved in intracellular redox signaling. -
Tissue specificity
Widely expressed. -
Sequence similarities
Belongs to the peroxiredoxin 2 family.
Contains 1 thioredoxin domain. -
Cellular localization
Mitochondrion. Cytoplasm. Peroxisome. - Information by UniProt
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Database links
- Entrez Gene: 25824 Human
- Entrez Gene: 54683 Mouse
- Entrez Gene: 113898 Rat
- Omim: 606583 Human
- SwissProt: P30044 Human
- SwissProt: P99029 Mouse
- SwissProt: Q9R063 Rat
- Unigene: 502823 Human
see all -
Alternative names
- ACR1 antibody
- Alu co repressor 1 antibody
- Alu corepressor 1 antibody
see all
Images
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Western blot - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] (ab180123)
Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: PRDX5 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HepG2 whole cell lysate (20 µg)
Lane 4: A549 whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab180123 observed at 17 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab180123 was shown to specifically react with PRDX5 when PRDX5 knockout samples were used. Wild-type and PRDX5 knockout samples were subjected to SDS-PAGE. Ab180123 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 10000 dilution and 1/10000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging. -
![Western blot - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] (ab180123)](https://www.abcam.com/ps/products/180/ab180123/Images/ab180123-209957-anti-peroxiredoxin-5-antibody-epr14528b-western-blot.jpg)
Western blot - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] (ab180123)All lanes : Anti-Peroxiredoxin 5 antibody [EPR14528(B)] (ab180123) at 1/20000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : A549 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 22 kDa
Additional bands at: 17 kDa. We are unsure as to the identity of these extra bands. -
![Immunocytochemistry/ Immunofluorescence - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] (ab180123)](https://www.abcam.com/ps/products/180/ab180123/Images/ab180123-209959-anti-peroxiredoxin-5-antibody-epr14528b-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] (ab180123)Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling Peroxiredoxin 5 with ab180123 at 1/250 dilution, followed by Goat anti rabbit IgG (Dylight 555) at 1/250 dilution. Counterstained with DAPI (blue).
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![Immunoprecipitation - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] (ab180123)](https://www.abcam.com/ps/products/180/ab180123/Images/ab180123-209961-anti-peroxiredoxin-5-antibody-epr14528b-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] (ab180123)Western Blot analysis of HeLa cell lysate immunoprecipitated with ab180123 at 1/60 dilution, using Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution.
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![Flow Cytometry (Intracellular) - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] (ab180123)](https://www.abcam.com/ps/products/180/ab180123/Images/ab180123-209960-anti-peroxiredoxin-5-antibody-epr14528b-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] (ab180123)Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling Peroxiredoxin 5with ab180123 at 1/10 dilution (red) compared toa rabbit IgG control (green), followed by Goat anti rabbit IgG (FITC) at 1/75 dilution.
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![Anti-Peroxiredoxin 5 antibody [EPR14528(B)] (ab180123)](https://www.abcam.com/ps/products/180/ab180123/Images/ab180123-6-benefits-of-recombinant-antibodies.png)
Anti-Peroxiredoxin 5 antibody [EPR14528(B)] (ab180123)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab180123 has been referenced in 1 publication.
- Lovatt M et al. Peroxiredoxin-1 regulates lipid peroxidation in corneal endothelial cells. Redox Biol 30:101417 (2020). PubMed: 31901729