Recombinant Anti-Peroxiredoxin 6 antibody [EPR3754] (ab133348)
![Western blot - Anti-Peroxiredoxin 6 antibody [EPR3754] (ab133348)](https://www.abcam.com/ps/products/133/ab133348/Images/ab133348-327970-ab133348KOWBDWAR.jpg "Western blot - Anti-Peroxiredoxin 6 antibody [EPR3754] (ab133348)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3754] to Peroxiredoxin 6
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
-
Product name
Anti-Peroxiredoxin 6 antibody [EPR3754]
See all Peroxiredoxin 6 primary antibodies -
Description
Rabbit monoclonal [EPR3754] to Peroxiredoxin 6 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Pig
-
Immunogen
Synthetic peptide within Human Peroxiredoxin 6 aa 50-150. The exact sequence is proprietary.
-
Positive control
- HAP1, HeLa, 293 and fetal liver lysates; Human brain tissue
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3754 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133348 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (2) |
1/1000 - 1/10000. Predicted molecular weight: 25 kDa.
|
| IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 25 kDa. |
|
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
-
Function
Involved in redox regulation of the cell. Can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. May play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury. -
Sequence similarities
Belongs to the ahpC/TSA family. Rehydrin subfamily.
Contains 1 thioredoxin domain. -
Cellular localization
Cytoplasm. Lysosome. Cytoplasmic vesicle. Also found in lung secretory organelles. - Information by UniProt
-
Database links
- Entrez Gene: 9588 Human
- Entrez Gene: 11758 Mouse
- Entrez Gene: 399538 Pig
- Entrez Gene: 94167 Rat
- Omim: 602316 Human
- SwissProt: P30041 Human
- SwissProt: O08709 Mouse
- SwissProt: Q9TSX9 Pig
see all -
Alternative names
- 1 Cys antibody
- 1 Cys peroxiredoxin antibody
- 1 Cys PRX antibody
see all
Images
-
Western blot - Anti-Peroxiredoxin 6 antibody [EPR3754] (ab133348)All lanes : Anti-Peroxiredoxin 6 antibody [EPR3754] (ab133348) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : PRDX6 (Peroxiredoxin 6) knockout HAP1 whole cell lysate
Lane 3 : A431 whole cell lysate
Lane 4 : PC3 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 25 kDaLanes 1 - 4: Merged signal (red and green). Green - ab133348 observed at 25 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab133348 was shown to specifically react with Peroxiredoxin 6 in wild-type HAP1 cells as signal was lost in PRDX6 (Peroxiredoxin 6) knockout cells. Wild-type and PRDX6 (Peroxiredoxin 6) knockout samples were subjected to SDS-PAGE. Ab133348 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 6 antibody [EPR3754] (ab133348)](https://www.abcam.com/ps/products/133/ab133348/Images/Peroxiredoxin-6-Primary-antibodies-ab133348-5.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 6 antibody [EPR3754] (ab133348)Immunohistochemical analysis of paraffin embedded normal Human stomach tissue using ab133348 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Western blot - Anti-Peroxiredoxin 6 antibody [EPR3754] (ab133348)](https://www.abcam.com/ps/products/133/ab133348/Images/ab133348-469583-anti-peroxiredoxin-6-antibody-epr3754-western-blot-wildtype-hap1-prdx6-knockout-hap1-human.jpg)
Western blot - Anti-Peroxiredoxin 6 antibody [EPR3754] (ab133348)All lanes : Anti-Peroxiredoxin 6 antibody [EPR3754] (ab133348) at 1/1000 dilution
Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : PRDX6 knockout HAP1 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 25 kDaab133348 was shown to react with PRDX6 in wild-type HAP1 cells in Western blot with loss of signal observed in a PRDX6 knockout cell line. Wild-type HAP1 and PRDX6 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab133348 overnight at 4 °C at a 1/1000 dilution. Blots were incubated with goat anti-rabbit HRP secondary antibodies at 0.2ug/mL before imaging. These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.
-
![Western blot - Anti-Peroxiredoxin 6 antibody [EPR3754] (ab133348)](https://www.abcam.com/ps/products/133/ab133348/Images/Peroxiredoxin-6-Primary-antibodies-ab133348-1.jpg)
Western blot - Anti-Peroxiredoxin 6 antibody [EPR3754] (ab133348)All lanes : Anti-Peroxiredoxin 6 antibody [EPR3754] (ab133348)
Lane 1 : HeLa lysate
Lane 2 : 293 lysate
Lane 3 : Fetal liver lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 25 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 6 antibody [EPR3754] (ab133348)](https://www.abcam.com/ps/products/133/ab133348/Images/Peroxiredoxin-6-Primary-antibodies-ab133348-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 6 antibody [EPR3754] (ab133348)Immunohistochemical analysis of paraffin-embedded Human brain tissue labelling Peroxiredoxin 6 with ab133348 at 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 6 antibody [EPR3754] (ab133348)](https://www.abcam.com/ps/products/133/ab133348/Images/Peroxiredoxin-6-Primary-antibodies-ab133348-3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 6 antibody [EPR3754] (ab133348)Immunohistochemical analysis of paraffin embedded normal Human liver tissue using ab133348 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 6 antibody [EPR3754] (ab133348)](https://www.abcam.com/ps/products/133/ab133348/Images/Peroxiredoxin-6-Primary-antibodies-ab133348-4.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 6 antibody [EPR3754] (ab133348)Immunohistochemical analysis of paraffin embedded normal Human placenta tissue using ab133348 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 6 antibody [EPR3754] (ab133348)](https://www.abcam.com/ps/products/133/ab133348/Images/ab133348-146905-anti-peroxiredoxin-6-antibody-epr3754-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 6 antibody [EPR3754] (ab133348)Immunohistochemical analysis of paraffin embedded normal Human testis tissue using ab133348 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Anti-Peroxiredoxin 6 antibody [EPR3754] (ab133348)](https://www.abcam.com/ps/products/133/ab133348/Images/ab133348-8-benefits-of-recombinant-antibodies.png)
Anti-Peroxiredoxin 6 antibody [EPR3754] (ab133348)
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (9)
ab133348 has been referenced in 9 publications.
- Li HT et al. Peroxiredoxin 6 mediates the protective function of curcumin pretreatment in acute lung injury induced by serum from patients undergoing one-lung ventilation in vitro. BMC Pulm Med 22:192 (2022). PubMed: 35549905
- López-Grueso MJ et al. Knockout of PRDX6 induces mitochondrial dysfunction and cell cycle arrest at G2/M in HepG2 hepatocarcinoma cells. Redox Biol 37:101737 (2020). PubMed: 33035814
- Scalabrin M et al. Redox responses in skeletal muscle following denervation. Redox Biol 26:101294 (2019). PubMed: 31450104
- Tang M et al. Hippocampal proteomic changes of susceptibility and resilience to depression or anxiety in a rat model of chronic mild stress. Transl Psychiatry 9:260 (2019). PubMed: 31624233
- Gumucio JP et al. Reduced mitochondrial lipid oxidation leads to fat accumulation in myosteatosis. FASEB J 33:7863-7881 (2019). PubMed: 30939247
- Li YL et al. Screening and identification of differentially expressed serum proteins in patients with vitiligo using two-dimensional gel electrophoresis coupled with mass spectrometry. Mol Med Rep 17:2651-2659 (2018). PubMed: 29207142
- Byun JM et al. Overexpression of peroxiredoxin-3 and -5 is a potential biomarker for prognosis in endometrial cancer. Oncol Lett 15:5111-5118 (2018). PubMed: 29541251
- Shanshan Y et al. Phospholipase A2 of Peroxiredoxin 6 Plays a Critical Role in Cerebral Ischemia/Reperfusion Inflammatory Injury. Front Cell Neurosci 11:99 (2017). WB . PubMed: 28424593
- Liu Y et al. Antifatigue Effects of Antrodia cinnamomea Cultured Mycelium via Modulation of Oxidative Stress Signaling in a Mouse Model. Biomed Res Int 2017:9374026 (2017). WB . PubMed: 28424791