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    products/primary-antibodies/peroxiredoxin-6-antibody-epr3754-ab133348.pdf

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Signal Transduction Metabolism Lipid metabolism
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Peroxiredoxin 6 antibody [EPR3754] (ab133348)

  • Datasheet
  • SDS
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Western blot - Anti-Peroxiredoxin 6 antibody [EPR3754] (ab133348)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 6 antibody [EPR3754] (ab133348)
  • Western blot - Anti-Peroxiredoxin 6 antibody [EPR3754] (ab133348)
  • Western blot - Anti-Peroxiredoxin 6 antibody [EPR3754] (ab133348)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 6 antibody [EPR3754] (ab133348)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 6 antibody [EPR3754] (ab133348)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 6 antibody [EPR3754] (ab133348)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 6 antibody [EPR3754] (ab133348)
  • Anti-Peroxiredoxin 6 antibody [EPR3754] (ab133348)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3754] to Peroxiredoxin 6
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Human

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Overview

  • Product name

    Anti-Peroxiredoxin 6 antibody [EPR3754]
    See all Peroxiredoxin 6 primary antibodies
  • Description

    Rabbit monoclonal [EPR3754] to Peroxiredoxin 6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Pig
  • Immunogen

    Synthetic peptide within Human Peroxiredoxin 6 aa 50-150. The exact sequence is proprietary.

  • Positive control

    • HAP1, HeLa, 293 and fetal liver lysates; Human brain tissue
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR3754
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Cell Biology
    • Other Antibodies
    • Oxidative Stress
    • Cardiovascular
    • Atherosclerosis
    • Ischemia / Reperfusion
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Inflammatory mediators
    • Cancer
    • Cancer Metabolism
    • Cellular metabolic process
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Oxidative stress
    • Metabolism
    • Types of disease
    • Heart disease

Associated products

  • Alternative Versions

    • PE Anti-Peroxiredoxin 6 antibody [EPR3754] (ab209008)
    • Anti-Peroxiredoxin 6 antibody [EPR3754] - BSA and Azide free (ab240057)
  • Compatible Secondaries

    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant human Peroxiredoxin 6 protein (ab87631)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab133348 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (2)
1/1000 - 1/10000. Predicted molecular weight: 25 kDa.
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 25 kDa.
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Application notes
Is unsuitable for IP.

Target

  • Function

    Involved in redox regulation of the cell. Can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. May play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury.
  • Sequence similarities

    Belongs to the ahpC/TSA family. Rehydrin subfamily.
    Contains 1 thioredoxin domain.
  • Cellular localization

    Cytoplasm. Lysosome. Cytoplasmic vesicle. Also found in lung secretory organelles.
  • Target information above from: UniProt accession P30041 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 9588 Human
    • Entrez Gene: 11758 Mouse
    • Entrez Gene: 399538 Pig
    • Entrez Gene: 94167 Rat
    • Omim: 602316 Human
    • SwissProt: P30041 Human
    • SwissProt: O08709 Mouse
    • SwissProt: Q9TSX9 Pig
    • SwissProt: O35244 Rat
    • Unigene: 120 Human
    • Unigene: 186185 Mouse
    • Unigene: 42 Rat
    see all
  • Alternative names

    • 1 Cys antibody
    • 1 Cys peroxiredoxin antibody
    • 1 Cys PRX antibody
    • 1 cysPrx antibody
    • 1-Cys peroxiredoxin antibody
    • 1-Cys PRX antibody
    • 24 kDa protein antibody
    • 9430088D19Rik antibody
    • AA690119 antibody
    • Acidic calcium independent phospholipase A2 antibody
    • Acidic calcium-independent phospholipase A2 antibody
    • aiPLA2 antibody
    • Antioxidant protein 2 antibody
    • AOP2 antibody
    • Aop2 rs3 antibody
    • Brp 12 antibody
    • Ciliary body glutathione peroxidase antibody
    • CP 3 antibody
    • EC 1.11.1.15 antibody
    • EC 1.11.1.7 antibody
    • EC 3.1.1. antibody
    • Epididymis secretory sperm binding protein Li 128m antibody
    • GPx antibody
    • HEL S 128m antibody
    • KIAA0106 antibody
    • Liver 2D page spot 40 antibody
    • Ltw4 antibody
    • Lvtw 4 antibody
    • MGC46173 antibody
    • mKIAA0106 antibody
    • Non selenium glutathione peroxidase antibody
    • Non-selenium glutathione peroxidase antibody
    • NSGPx antibody
    • ORF06 antibody
    • OTTHUMP00000032693 antibody
    • p29 antibody
    • Peroxiredoxin-6 antibody
    • Peroxiredoxin6 antibody
    • PHGPx antibody
    • Phospholipase A2 lysosomal antibody
    • PLA2 antibody
    • PRDX 6 antibody
    • Prdx5 antibody
    • PRDX6 antibody
    • Prdx6 rs3 antibody
    • PRDX6_HUMAN antibody
    • PRX antibody
    • Red blood cells page spot 12 antibody
    • Thiol specific antioxidant protein antibody
    see all

Images

  • Western blot - Anti-Peroxiredoxin 6 antibody [EPR3754] (ab133348)
    Western blot - Anti-Peroxiredoxin 6 antibody [EPR3754] (ab133348)
    All lanes : Anti-Peroxiredoxin 6 antibody [EPR3754] (ab133348) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : PRDX6 (Peroxiredoxin 6) knockout HAP1 whole cell lysate
    Lane 3 : A431 whole cell lysate
    Lane 4 : PC3 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 25 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab133348 observed at 25 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab133348 was shown to specifically react with Peroxiredoxin 6 in wild-type HAP1 cells as signal was lost in PRDX6 (Peroxiredoxin 6) knockout cells. Wild-type and PRDX6 (Peroxiredoxin 6) knockout samples were subjected to SDS-PAGE. Ab133348 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 6 antibody [EPR3754] (ab133348)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 6 antibody [EPR3754] (ab133348)

    Immunohistochemical analysis of paraffin embedded normal Human stomach tissue using ab133348 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-Peroxiredoxin 6 antibody [EPR3754] (ab133348)
    Western blot - Anti-Peroxiredoxin 6 antibody [EPR3754] (ab133348)
    All lanes : Anti-Peroxiredoxin 6 antibody [EPR3754] (ab133348) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 cell lysate
    Lane 2 : PRDX6 knockout HAP1 cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 25 kDa



    ab133348 was shown to react with PRDX6 in wild-type HAP1 cells in Western blot with loss of signal observed in a PRDX6 knockout cell line. Wild-type HAP1 and PRDX6 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab133348 overnight at 4 °C at a 1/1000 dilution. Blots were incubated with goat anti-rabbit HRP secondary antibodies at 0.2ug/mL before imaging. These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

  • Western blot - Anti-Peroxiredoxin 6 antibody [EPR3754] (ab133348)
    Western blot - Anti-Peroxiredoxin 6 antibody [EPR3754] (ab133348)
    All lanes : Anti-Peroxiredoxin 6 antibody [EPR3754] (ab133348)

    Lane 1 : HeLa lysate
    Lane 2 : 293 lysate
    Lane 3 : Fetal liver lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 25 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 6 antibody [EPR3754] (ab133348)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 6 antibody [EPR3754] (ab133348)

    Immunohistochemical analysis of paraffin-embedded Human brain tissue labelling Peroxiredoxin 6 with ab133348 at 1/250 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 6 antibody [EPR3754] (ab133348)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 6 antibody [EPR3754] (ab133348)

    Immunohistochemical analysis of paraffin embedded normal Human liver tissue using ab133348 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 6 antibody [EPR3754] (ab133348)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 6 antibody [EPR3754] (ab133348)

    Immunohistochemical analysis of paraffin embedded normal Human placenta tissue using ab133348 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 6 antibody [EPR3754] (ab133348)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 6 antibody [EPR3754] (ab133348)

    Immunohistochemical analysis of paraffin embedded normal Human testis tissue using ab133348 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-Peroxiredoxin 6 antibody [EPR3754] (ab133348)
    Anti-Peroxiredoxin 6 antibody [EPR3754] (ab133348)

Protocols

  • Immunohistochemistry protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (8)

Publishing research using ab133348? Please let us know so that we can cite the reference in this datasheet.

ab133348 has been referenced in 8 publications.

  • López-Grueso MJ  et al. Knockout of PRDX6 induces mitochondrial dysfunction and cell cycle arrest at G2/M in HepG2 hepatocarcinoma cells. Redox Biol 37:101737 (2020). PubMed: 33035814
  • Scalabrin M  et al. Redox responses in skeletal muscle following denervation. Redox Biol 26:101294 (2019). PubMed: 31450104
  • Tang M  et al. Hippocampal proteomic changes of susceptibility and resilience to depression or anxiety in a rat model of chronic mild stress. Transl Psychiatry 9:260 (2019). PubMed: 31624233
  • Gumucio JP  et al. Reduced mitochondrial lipid oxidation leads to fat accumulation in myosteatosis. FASEB J 33:7863-7881 (2019). PubMed: 30939247
  • Li YL  et al. Screening and identification of differentially expressed serum proteins in patients with vitiligo using two-dimensional gel electrophoresis coupled with mass spectrometry. Mol Med Rep 17:2651-2659 (2018). PubMed: 29207142
  • Byun JM  et al. Overexpression of peroxiredoxin-3 and -5 is a potential biomarker for prognosis in endometrial cancer. Oncol Lett 15:5111-5118 (2018). PubMed: 29541251
  • Shanshan Y  et al. Phospholipase A2 of Peroxiredoxin 6 Plays a Critical Role in Cerebral Ischemia/Reperfusion Inflammatory Injury. Front Cell Neurosci 11:99 (2017). WB . PubMed: 28424593
  • Liu Y  et al. Antifatigue Effects of Antrodia cinnamomea Cultured Mycelium via Modulation of Oxidative Stress Signaling in a Mouse Model. Biomed Res Int 2017:9374026 (2017). WB . PubMed: 28424791

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Western blot abreview for Anti-Peroxiredoxin 6 antibody [EPR3754]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (liver)
Gel Running Conditions
Non-reduced Denaturing (12%)
Loading amount
30 µg
Specification
liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jun 30 2017

Western blot abreview for Anti-Peroxiredoxin 6 antibody [EPR3754]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
62.5 µg
Gel Running Conditions
Reduced Denaturing (12.5% acrylamide)
Sample
Pig Tissue lysate - other (Muscle sarcoplasmic extract)
Specification
Muscle sarcoplasmic extract
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. Shannon Cruzen

Verified customer

Submitted Feb 05 2014

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