Recombinant Anti-PEX19 antibody [EPR9266(B)] (ab137072)
![Western blot - Anti-PEX19 antibody [EPR9266(B)] (ab137072)](https://www.abcam.com/ps/products/137/ab137072/Images/ab137072-310242-anti-pex19-antibody-epr9266b-western-blot.jpg "Western blot - Anti-PEX19 antibody [EPR9266(B)] (ab137072)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9266(B)] to PEX19
- Suitable for: Flow Cyt (Intra), IP, ICC/IF, WB
- Reacts with: Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-PEX19 antibody [EPR9266(B)]
See all PEX19 primary antibodies -
Description
Rabbit monoclonal [EPR9266(B)] to PEX19 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), IP, ICC/IF, WBmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic peptide within Human PEX19 aa 1-100. The exact sequence is proprietary.
Database link: P40855 -
Positive control
- Jurkat, rat brain lysate, rat heart and MOLT4 cell lysates; Jurkat cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9266(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137072 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| IP |
1/10 - 1/100.
|
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| ICC/IF |
1/50 - 1/100.
|
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| WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 33 kDa.
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| Notes |
|---|
|
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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IP
1/10 - 1/100. |
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ICC/IF
1/50 - 1/100. |
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WB
1/1000 - 1/10000. Predicted molecular weight: 33 kDa. |
Target
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Function
Necessary for early peroxisomal biogenesis. Acts both as a cytosolic chaperone and as an import receptor for peroxisomal membrane proteins (PMPs). Binds and stabilizes newly synthesized PMPs in the cytoplasm by interacting with their hydrophobic membrane-spanning domains, and targets them to the peroxisome membrane by binding to the integral membrane protein PEX3. Excludes CDKN2A from the nucleus and prevents its interaction with MDM2, which results in active degradation of TP53. -
Tissue specificity
Ubiquitously expressed. Isoform 1 is strongly predominant in all tissues except in utero where isoform 2 is the main form. -
Involvement in disease
Defects in PEX19 are the cause of peroxisome biogenesis disorder complementation group 14 (PBD-CG14) [MIM:600279]; also known as PBD-CGJ. PBD refers to a group of peroxisomal disorders arising from a failure of protein import into the peroxisomal membrane or matrix. The PBD group is comprised of four disorders: Zellweger syndrome (ZWS), neonatal adrenoleukodystrophy (NALD), infantile Refsum disease (IRD), and classical rhizomelic chondrodysplasia punctata (RCDP). ZWS, NALD and IRD are distinct from RCDP and constitute a clinical continuum of overlapping phenotypes known as the Zellweger spectrum. The PBD group is genetically heterogeneous with at least 14 distinct genetic groups as concluded from complementation studies.
Defects in PEX19 are a cause of Zellweger syndrome (ZWS) [MIM:214100]. ZWS is a fatal peroxisome biogenesis disorder characterized by dysmorphic facial features, hepatomegaly, ocular abnormalities, renal cysts, hearing impairment, profound psychomotor retardation, severe hypotonia and neonatal seizures. Death occurs within the first year of life. -
Sequence similarities
Belongs to the peroxin-19 family. -
Cellular localization
Cytoplasm. Peroxisome membrane. Mainly cytoplasmic. Some fraction membrane-associated to the outer surface of peroxisomes. - Information by UniProt
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Database links
- Entrez Gene: 5824 Human
- Entrez Gene: 289233 Rat
- Omim: 600279 Human
- SwissProt: P40855 Human
- SwissProt: Q9QYU1 Rat
- Unigene: 517232 Human
- Unigene: 203104 Rat
- Unigene: 225447 Rat
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Alternative names
- 33 kDa housekeeping protein antibody
- D1S2223E antibody
- HK33 antibody
see all
Images
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Western blot - Anti-PEX19 antibody [EPR9266(B)] (ab137072)All lanes : Anti-PEX19 antibody [EPR9266(B)] (ab137072) at 1/1000 dilution (Purified)
Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates with 5% NFDM/TBST
Lane 2 : MOLT-4 (Human lymphoblastic leukemia T lymphoblast) whole cell lysates with 5% NFDM/TBST
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 33 kDa
Observed band size: 35,40 kDa why is the actual band size different from the predicted?
The doublets are reported by PMID: 9339377, 28817674 and 10051604 -
![Immunocytochemistry/ Immunofluorescence - Anti-PEX19 antibody [EPR9266(B)] (ab137072)](https://www.abcam.com/ps/products/137/ab137072/Images/ab137072-310262-anti-pex19-antibody-epr9266b-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PEX19 antibody [EPR9266(B)] (ab137072)Immunocytochemistry/ Immunofluorescence analysis of MOLT-4 (Human lymphoblastic leukemia T lymphoblast) cells labeling PEX19 with Purified ab137072 at 1:100 (10.2 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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![Flow Cytometry (Intracellular) - Anti-PEX19 antibody [EPR9266(B)] (ab137072)](https://www.abcam.com/ps/products/137/ab137072/Images/ab137072-310267-anti-pex19-antibody-epr9266b-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-PEX19 antibody [EPR9266(B)] (ab137072)Intracellular Flow Cytometry analysis of A549 (Human lung carcinoma epithelial cell) cells labeling PEX19 with purified ab137072 at 1/100 dilution (10µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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![Western blot - Anti-PEX19 antibody [EPR9266(B)] (ab137072)](https://www.abcam.com/ps/products/137/ab137072/Images/ab137072-310241-anti-pex19-antibody-epr9266b-western-blot.jpg)
Western blot - Anti-PEX19 antibody [EPR9266(B)] (ab137072)All lanes : Anti-PEX19 antibody [EPR9266(B)] (ab137072) at 1/2000 dilution (Purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates with 5% NFDM/TBST
Lane 2 : Rat brain lysates with 5% NFDM/TBST
Lane 3 : Rat heart lysates with 5% NFDM/TBST
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 33 kDa
Observed band size: 35,40 kDa why is the actual band size different from the predicted?
The doublets are reported by PMID: 9339377, 28817674 and 10051604 -
![Immunoprecipitation - Anti-PEX19 antibody [EPR9266(B)] (ab137072)](https://www.abcam.com/ps/products/137/ab137072/Images/ab137072-310275-anti-pex19-antibody-epr9266b-immunoprecipitation.jpg)
Immunoprecipitation - Anti-PEX19 antibody [EPR9266(B)] (ab137072)ab137072 (purified) at 1:50 dilution (2µg) immunoprecipitating PEX19 in MOLT-4 whole cell lysate.
Lane 1 (input): MOLT-4 (Human lymphoblastic leukemia T lymphoblast) whole cell lysate 10µg
Lane 2 (+): ab137072 & MOLT-4 whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab137072 in MOLT-4 whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST. -
![Anti-PEX19 antibody [EPR9266(B)] (ab137072)](https://www.abcam.com/ps/products/137/ab137072/Images/ab137072-8-benefits-of-recombinant-antibodies.png)
Anti-PEX19 antibody [EPR9266(B)] (ab137072)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (15)
ab137072 has been referenced in 15 publications.
- Farelo MA et al. Dengue and Zika Virus Capsid Proteins Contain a Common PEX19-Binding Motif. Viruses 14:N/A (2022). PubMed: 35215846
- Lyschik S et al. PEX19 Coordinates Neutral Lipid Storage in Cells in a Peroxisome-Independent Fashion. Front Cell Dev Biol 10:859052 (2022). PubMed: 35557938
- Huang TY et al. Combined effects of a ketogenic diet and exercise training alter mitochondrial and peroxisomal substrate oxidative capacity in skeletal muscle. Am J Physiol Endocrinol Metab 320:E1053-E1067 (2021). PubMed: 33843280
- Aleksic M et al. Hypothyroidism Intensifies Both Canonic and the De Novo Pathway of Peroxisomal Biogenesis in Rat Brown Adipocytes in a Time-Dependent Manner. Cells 10:N/A (2021). PubMed: 34571897
- Huang TY et al. Response of Liver Metabolic Pathways to Ketogenic Diet and Exercise Are Not Additive. Med Sci Sports Exerc 52:37-48 (2020). PubMed: 31389908