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    products/primary-antibodies/pex19-antibody-epr9266b-ab137072.pdf

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RecombinantRabMAb

Recombinant Anti-PEX19 antibody [EPR9266(B)] (ab137072)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (15)

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Western blot - Anti-PEX19 antibody [EPR9266(B)] (ab137072)
  • Immunocytochemistry/ Immunofluorescence - Anti-PEX19 antibody [EPR9266(B)] (ab137072)
  • Flow Cytometry (Intracellular) - Anti-PEX19 antibody [EPR9266(B)] (ab137072)
  • Western blot - Anti-PEX19 antibody [EPR9266(B)] (ab137072)
  • Immunoprecipitation - Anti-PEX19 antibody [EPR9266(B)] (ab137072)
  • Anti-PEX19 antibody [EPR9266(B)] (ab137072)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9266(B)] to PEX19
  • Suitable for: Flow Cyt (Intra), IP, ICC/IF, WB
  • Reacts with: Rat, Human

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Carrier Free

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Overview

  • Product name

    Anti-PEX19 antibody [EPR9266(B)]
    See all PEX19 primary antibodies
  • Description

    Rabbit monoclonal [EPR9266(B)] to PEX19
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), IP, ICC/IF, WBmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide within Human PEX19 aa 1-100. The exact sequence is proprietary.
    Database link: P40855

  • Positive control

    • Jurkat, rat brain lysate, rat heart and MOLT4 cell lysates; Jurkat cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR9266(B)
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Peroxisome
    • Signal Transduction
    • Protein Trafficking
    • Organelle Proteins

Associated products

  • Alternative Versions

    • Anti-PEX19 antibody [EPR9266(B)] - BSA and Azide free (ab232421)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Jurkat cytoplasmic extract lysate (ab14842)
    • Rat brain normal tissue lysate - membrane extract (ab29473)
    • Jurkat membrane extract lysate (ab30130)
  • Recombinant Protein

    • Recombinant Human PEX19 protein (ab111623)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab137072 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP
1/10 - 1/100.
ICC/IF
1/50 - 1/100.
WB (1)
1/1000 - 1/10000. Predicted molecular weight: 33 kDa.
Notes
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP
1/10 - 1/100.
ICC/IF
1/50 - 1/100.
WB
1/1000 - 1/10000. Predicted molecular weight: 33 kDa.
Application notes
Is unsuitable for IHC-P.

Target

  • Function

    Necessary for early peroxisomal biogenesis. Acts both as a cytosolic chaperone and as an import receptor for peroxisomal membrane proteins (PMPs). Binds and stabilizes newly synthesized PMPs in the cytoplasm by interacting with their hydrophobic membrane-spanning domains, and targets them to the peroxisome membrane by binding to the integral membrane protein PEX3. Excludes CDKN2A from the nucleus and prevents its interaction with MDM2, which results in active degradation of TP53.
  • Tissue specificity

    Ubiquitously expressed. Isoform 1 is strongly predominant in all tissues except in utero where isoform 2 is the main form.
  • Involvement in disease

    Defects in PEX19 are the cause of peroxisome biogenesis disorder complementation group 14 (PBD-CG14) [MIM:600279]; also known as PBD-CGJ. PBD refers to a group of peroxisomal disorders arising from a failure of protein import into the peroxisomal membrane or matrix. The PBD group is comprised of four disorders: Zellweger syndrome (ZWS), neonatal adrenoleukodystrophy (NALD), infantile Refsum disease (IRD), and classical rhizomelic chondrodysplasia punctata (RCDP). ZWS, NALD and IRD are distinct from RCDP and constitute a clinical continuum of overlapping phenotypes known as the Zellweger spectrum. The PBD group is genetically heterogeneous with at least 14 distinct genetic groups as concluded from complementation studies.
    Defects in PEX19 are a cause of Zellweger syndrome (ZWS) [MIM:214100]. ZWS is a fatal peroxisome biogenesis disorder characterized by dysmorphic facial features, hepatomegaly, ocular abnormalities, renal cysts, hearing impairment, profound psychomotor retardation, severe hypotonia and neonatal seizures. Death occurs within the first year of life.
  • Sequence similarities

    Belongs to the peroxin-19 family.
  • Cellular localization

    Cytoplasm. Peroxisome membrane. Mainly cytoplasmic. Some fraction membrane-associated to the outer surface of peroxisomes.
  • Target information above from: UniProt accession P40855 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5824 Human
    • Entrez Gene: 289233 Rat
    • Omim: 600279 Human
    • SwissProt: P40855 Human
    • SwissProt: Q9QYU1 Rat
    • Unigene: 517232 Human
    • Unigene: 203104 Rat
    • Unigene: 225447 Rat
    • Alternative names

      • 33 kDa housekeeping protein antibody
      • D1S2223E antibody
      • HK33 antibody
      • Housekeeping gene 33kD antibody
      • OK/SW-cl.22 antibody
      • PBD12A antibody
      • Peroxin 19 antibody
      • Peroxin-19 antibody
      • Peroxisomal biogenesis factor 19 antibody
      • Peroxisomal farnesylated protein antibody
      • PEX19 antibody
      • PEX19_HUMAN antibody
      • PMP1 antibody
      • PMPI antibody
      • PXF antibody
      • PXMP1 antibody
      see all

    Images

    • Western blot - Anti-PEX19 antibody [EPR9266(B)] (ab137072)
      Western blot - Anti-PEX19 antibody [EPR9266(B)] (ab137072)
      All lanes : Anti-PEX19 antibody [EPR9266(B)] (ab137072) at 1/1000 dilution (Purified)

      Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates with 5% NFDM/TBST
      Lane 2 : MOLT-4 (Human lymphoblastic leukemia T lymphoblast) whole cell lysates with 5% NFDM/TBST

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Predicted band size: 33 kDa
      Observed band size: 35,40 kDa why is the actual band size different from the predicted?



      The doublets are reported by PMID: 9339377, 28817674 and 10051604
    • Immunocytochemistry/ Immunofluorescence - Anti-PEX19 antibody [EPR9266(B)] (ab137072)
      Immunocytochemistry/ Immunofluorescence - Anti-PEX19 antibody [EPR9266(B)] (ab137072)

      Immunocytochemistry/ Immunofluorescence analysis of MOLT-4 (Human lymphoblastic leukemia T lymphoblast) cells labeling PEX19 with Purified ab137072 at 1:100 (10.2 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    • Flow Cytometry (Intracellular) - Anti-PEX19 antibody [EPR9266(B)] (ab137072)
      Flow Cytometry (Intracellular) - Anti-PEX19 antibody [EPR9266(B)] (ab137072)

      Intracellular Flow Cytometry analysis of A549 (Human lung carcinoma epithelial cell) cells labeling PEX19 with purified ab137072 at 1/100 dilution (10µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). 

       

       

    • Western blot - Anti-PEX19 antibody [EPR9266(B)] (ab137072)
      Western blot - Anti-PEX19 antibody [EPR9266(B)] (ab137072)
      All lanes : Anti-PEX19 antibody [EPR9266(B)] (ab137072) at 1/2000 dilution (Purified)

      Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates with 5% NFDM/TBST
      Lane 2 : Rat brain lysates with 5% NFDM/TBST
      Lane 3 : Rat heart lysates with 5% NFDM/TBST

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 33 kDa
      Observed band size: 35,40 kDa why is the actual band size different from the predicted?



      The doublets are reported by PMID: 9339377, 28817674 and 10051604
    • Immunoprecipitation - Anti-PEX19 antibody [EPR9266(B)] (ab137072)
      Immunoprecipitation - Anti-PEX19 antibody [EPR9266(B)] (ab137072)

      ab137072 (purified) at 1:50 dilution (2µg) immunoprecipitating PEX19 in MOLT-4 whole cell lysate.
      Lane 1 (input): MOLT-4 (Human lymphoblastic leukemia T lymphoblast) whole cell lysate 10µg
      Lane 2 (+): ab137072 & MOLT-4 whole cell lysate
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab137072 in MOLT-4 whole cell lysate
      For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
      Blocking and diluting buffer: 5% NFDM/TBST.

    • Anti-PEX19 antibody [EPR9266(B)] (ab137072)
      Anti-PEX19 antibody [EPR9266(B)] (ab137072)

    Protocols

    • Flow cytometry protocols
    • Immunoprecipitation protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (15)

    Publishing research using ab137072? Please let us know so that we can cite the reference in this datasheet.

    ab137072 has been referenced in 15 publications.

    • Farelo MA  et al. Dengue and Zika Virus Capsid Proteins Contain a Common PEX19-Binding Motif. Viruses 14:N/A (2022). PubMed: 35215846
    • Lyschik S  et al. PEX19 Coordinates Neutral Lipid Storage in Cells in a Peroxisome-Independent Fashion. Front Cell Dev Biol 10:859052 (2022). PubMed: 35557938
    • Huang TY  et al. Combined effects of a ketogenic diet and exercise training alter mitochondrial and peroxisomal substrate oxidative capacity in skeletal muscle. Am J Physiol Endocrinol Metab 320:E1053-E1067 (2021). PubMed: 33843280
    • Aleksic M  et al. Hypothyroidism Intensifies Both Canonic and the De Novo Pathway of Peroxisomal Biogenesis in Rat Brown Adipocytes in a Time-Dependent Manner. Cells 10:N/A (2021). PubMed: 34571897
    • Huang TY  et al. Response of Liver Metabolic Pathways to Ketogenic Diet and Exercise Are Not Additive. Med Sci Sports Exerc 52:37-48 (2020). PubMed: 31389908
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Western blot abreview for Anti-PEX19 antibody [EPR9266(B)]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEK293T cells)
    Gel Running Conditions
    Reduced Denaturing (5% stacking and 10% separating Bis-Tris gel)
    Loading amount
    270000 cells
    Specification
    HEK293T cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MS. Tamara Somborac

    Verified customer

    Submitted Feb 16 2018

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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