Recombinant Anti-PGC1 beta antibody [EPR12370] (ab176328)
![Western blot - Anti-PGC1 beta antibody [EPR12370] (ab176328)](https://www.abcam.com/ps/products/176/ab176328/Images/ab176328-257234-anti-pgc1-beta-antibody-epr12370-western-blot.jpg "Western blot - Anti-PGC1 beta antibody [EPR12370] (ab176328)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12370] to PGC1 beta
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
-
Product name
Anti-PGC1 beta antibody [EPR12370]
See all PGC1 beta primary antibodies -
Description
Rabbit monoclonal [EPR12370] to PGC1 beta -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: HeLa, MCF-7 and SH-SY5Y cell lysates and human fetal brain, human fetal heart, rat spleen and mouse spleen tissue lysates. IP: HeLa whole cell lysate (ab150035).
-
General notes
This product may be covered by one or more of the following U.S. Patents.
6,166,192
6,426,411
6,525,178
6,872,391
6,908,987
7,250,283
7,091,006
7,354,738
8,105,774
8,445,233This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.5% BSA, 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12370 -
Isotype
IgG -
Research areas
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
- Co-activators/co-repressors
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Fatty acids
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab176328 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (3) |
1/1000 - 1/10000. Predicted molecular weight: 113 kDa.
|
| IP |
1/10 - 1/100.
|
| Notes |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 113 kDa. |
|
IP
1/10 - 1/100. |
Target
-
Function
Plays a role of stimulator of transcription factors and nuclear receptors activities. Activates transcritional activity of estrogen receptor alpha, nuclear respiratory factor 1 (NRF1) and glucocorticoid receptor in the presence of glucocorticoids. May play a role in constitutive non-adrenergic-mediated mitochondrial biogenesis as suggested by increased basal oxygen consumption and mitochondrial number when overexpressed. May be involved in fat oxidation and non-oxidative glucose metabolism and in the regulation of energy expenditure. Induces the expression of PERM1 in the skeletal muscle in an ESRRA-dependent manner. -
Tissue specificity
Ubiquitous with higher expression in heart, brain and skeletal muscle. -
Sequence similarities
Contains 1 RRM (RNA recognition motif) domain. -
Domain
Contains 2 Leu-Xaa-Xaa-Leu-Leu (LXXLL) motif, which are usually required for the association with nuclear receptors. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 133522 Human
- Entrez Gene: 170826 Mouse
- Entrez Gene: 291567 Rat
- Omim: 608886 Human
- SwissProt: Q86YN6 Human
- SwissProt: Q8VHJ7 Mouse
- SwissProt: Q811R2 Rat
- Unigene: 483816 Human
see all -
Alternative names
- PERC antibody
- peroxisome proliferative activated receptor, gamma, coactivator 1, antibody
- peroxisome proliferator-activated receptor gamma coactivator 1 beta antibody
see all
Images
-
Western blot - Anti-PGC1 beta antibody [EPR12370] (ab176328)All lanes : Anti-PGC1 beta antibody [EPR12370] (ab176328) at 1/10000 dilution (purified)
Lane 1 : HeLa whole cell lysate
Lane 2 : MCF-7 whole cell lysate
Lane 3 : SH-SY5Y whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 113 kDa
Observed band size: 113 kDa
Blocking and dilution buffer: 5% NFDM/TBST. -
![Western blot - Anti-PGC1 beta antibody [EPR12370] (ab176328)](https://www.abcam.com/ps/products/176/ab176328/Images/ab176328-257235-anti-pgc1-beta-antibody-epr12370-western-blot.jpg)
Western blot - Anti-PGC1 beta antibody [EPR12370] (ab176328)All lanes : Anti-PGC1 beta antibody [EPR12370] (ab176328) at 1/10000 dilution (purified)
Lane 1 : Mouse spleen tissue lysate
Lane 2 : Rat spleen tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 113 kDa
Observed band size: 113 kDa
Blocking and dilution buffer: 5% NFDM/TBST. -
![Immunoprecipitation - Anti-PGC1 beta antibody [EPR12370] (ab176328)](https://www.abcam.com/ps/products/176/ab176328/Images/ab176328-257199-anti-pgc1-beta-antibody-epr12370-immunoprecipitation.jpg)
Immunoprecipitation - Anti-PGC1 beta antibody [EPR12370] (ab176328)ab176328 (purified) at 1/20 immunoprecipitating PGC1 beta in HeLa whole cell lysate.
Lane 1 (input): HeLa whole cell lysate (10µg)
Lane 2 (+): ab176328 + HeLa whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab176328 in HeLa whole cell lysate.
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
-
![Western blot - Anti-PGC1 beta antibody [EPR12370] (ab176328)](https://www.abcam.com/ps/products/176/ab176328/Images/ab176328-204140-anti-pgc1-beta-antibody-epr12370-western-blot.jpg)
Western blot - Anti-PGC1 beta antibody [EPR12370] (ab176328)All lanes : Anti-PGC1 beta antibody [EPR12370] (ab176328) at 1/1000 dilution (unpurified)
Lane 1 : HeLa cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : Human fetal brain tissue lysate
Lane 4 : Human fetal heart tissue lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 113 kDa -
![Immunoprecipitation - Anti-PGC1 beta antibody [EPR12370] (ab176328)](https://www.abcam.com/ps/products/176/ab176328/Images/ab176328-204143-anti-pgc1-beta-antibody-epr12370-immunoprecipitation.jpg)
Immunoprecipitation - Anti-PGC1 beta antibody [EPR12370] (ab176328)Western blot analysis on immunoprecipitation pellet from HeLa cell lysate labeling PGC1 beta using unpurified ab176328 at a dilution of 1/10.
-
![Anti-PGC1 beta antibody [EPR12370] (ab176328)](https://www.abcam.com/ps/products/176/ab176328/Images/ab176328-8-benefits-of-recombinant-antibodies.png)
Anti-PGC1 beta antibody [EPR12370] (ab176328)
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (34)
ab176328 has been referenced in 34 publications.
- Dumauthioz N et al. Enforced PGC-1a expression promotes CD8 T cell fitness, memory formation and antitumor immunity. Cell Mol Immunol 18:1761-1771 (2021). PubMed: 32055005
- Campelj DG et al. Metronomic 5-Fluorouracil Delivery Primes Skeletal Muscle for Myopathy but Does Not Cause Cachexia. Pharmaceuticals (Basel) 14:N/A (2021). PubMed: 34067869
- Sen T et al. Decreased PGC1ß expression results in disrupted human erythroid differentiation, impaired hemoglobinization and cell cycle exit. Sci Rep 11:17129 (2021). PubMed: 34429458
- Zheng ZG et al. Dual targeting of SREBP2 and ERRa by carnosic acid suppresses RANKL-mediated osteoclastogenesis and prevents ovariectomy-induced bone loss. Cell Death Differ 27:2048-2065 (2020). PubMed: 31907393
- Sári Z et al. Indolepropionic Acid, a Metabolite of the Microbiome, Has Cytostatic Properties in Breast Cancer by Activating AHR and PXR Receptors and Inducing Oxidative Stress. Cancers (Basel) 12:N/A (2020). PubMed: 32854297