Recombinant Anti-PGC1 beta antibody [EPR12370] (ab176328)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12370] to PGC1 beta
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-PGC1 beta antibody [EPR12370]
See all PGC1 beta primary antibodies -
Description
Rabbit monoclonal [EPR12370] to PGC1 beta -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, MCF-7 and SH-SY5Y cell lysates and human fetal brain, human fetal heart, rat spleen and mouse spleen tissue lysates. IP: HeLa whole cell lysate (ab150035).
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General notes
This product may be covered by one or more of the following U.S. Patents.
6,166,192
6,426,411
6,525,178
6,872,391
6,908,987
7,250,283
7,091,006
7,354,738
8,105,774
8,445,233This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.5% BSA, 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12370 -
Isotype
IgG -
Research areas
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
- Co-activators/co-repressors
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Fatty acids
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab176328 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (3) |
1/1000 - 1/10000. Predicted molecular weight: 113 kDa.
|
IP |
1/10 - 1/100.
|
Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 113 kDa. |
IP
1/10 - 1/100. |
Target
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Function
Plays a role of stimulator of transcription factors and nuclear receptors activities. Activates transcritional activity of estrogen receptor alpha, nuclear respiratory factor 1 (NRF1) and glucocorticoid receptor in the presence of glucocorticoids. May play a role in constitutive non-adrenergic-mediated mitochondrial biogenesis as suggested by increased basal oxygen consumption and mitochondrial number when overexpressed. May be involved in fat oxidation and non-oxidative glucose metabolism and in the regulation of energy expenditure. Induces the expression of PERM1 in the skeletal muscle in an ESRRA-dependent manner. -
Tissue specificity
Ubiquitous with higher expression in heart, brain and skeletal muscle. -
Sequence similarities
Contains 1 RRM (RNA recognition motif) domain. -
Domain
Contains 2 Leu-Xaa-Xaa-Leu-Leu (LXXLL) motif, which are usually required for the association with nuclear receptors. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 133522 Human
- Entrez Gene: 170826 Mouse
- Entrez Gene: 291567 Rat
- Omim: 608886 Human
- SwissProt: Q86YN6 Human
- SwissProt: Q8VHJ7 Mouse
- SwissProt: Q811R2 Rat
- Unigene: 483816 Human
see all -
Alternative names
- PERC antibody
- peroxisome proliferative activated receptor, gamma, coactivator 1, antibody
- peroxisome proliferator-activated receptor gamma coactivator 1 beta antibody
see all
Images
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All lanes : Anti-PGC1 beta antibody [EPR12370] (ab176328) at 1/10000 dilution (purified)
Lane 1 : HeLa whole cell lysate
Lane 2 : MCF-7 whole cell lysate
Lane 3 : SH-SY5Y whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 113 kDa
Observed band size: 113 kDa
Blocking and dilution buffer: 5% NFDM/TBST. -
All lanes : Anti-PGC1 beta antibody [EPR12370] (ab176328) at 1/10000 dilution (purified)
Lane 1 : Mouse spleen tissue lysate
Lane 2 : Rat spleen tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 113 kDa
Observed band size: 113 kDa
Blocking and dilution buffer: 5% NFDM/TBST. -
ab176328 (purified) at 1/20 immunoprecipitating PGC1 beta in HeLa whole cell lysate.
Lane 1 (input): HeLa whole cell lysate (10µg)
Lane 2 (+): ab176328 + HeLa whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab176328 in HeLa whole cell lysate.
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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All lanes : Anti-PGC1 beta antibody [EPR12370] (ab176328) at 1/1000 dilution (unpurified)
Lane 1 : HeLa cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : Human fetal brain tissue lysate
Lane 4 : Human fetal heart tissue lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 113 kDa -
Western blot analysis on immunoprecipitation pellet from HeLa cell lysate labeling PGC1 beta using unpurified ab176328 at a dilution of 1/10.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (40)
ab176328 has been referenced in 40 publications.
- Ghilardi C et al. PGC1α/β Expression Predicts Therapeutic Response to Oxidative Phosphorylation Inhibition in Ovarian Cancer. Cancer Res 82:1423-1434 (2022). PubMed: 35131872
- Xu Z et al. The E3 ubiquitin ligase RBCK1 promotes the invasion and metastasis of hepatocellular carcinoma by destroying the PPARγ/PGC1α complex. Am J Cancer Res 12:1372-1392 (2022). PubMed: 35411229
- Lee GR et al. Dynein light chain LC8 alleviates nonalcoholic steatohepatitis by inhibiting NF-κB signaling and reducing oxidative stress. J Cell Physiol 237:3554-3564 (2022). PubMed: 35696549
- Campelj DG et al. Metronomic 5-Fluorouracil Delivery Primes Skeletal Muscle for Myopathy but Does Not Cause Cachexia. Pharmaceuticals (Basel) 14:N/A (2021). PubMed: 34067869
- Dumauthioz N et al. Enforced PGC-1a expression promotes CD8 T cell fitness, memory formation and antitumor immunity. Cell Mol Immunol 18:1761-1771 (2021). PubMed: 32055005