Anti-PGK1 antibody [22C5D8] (ab113687)
![Western blot - Anti-PGK1 antibody [22C5D8] (ab113687)](https://www.abcam.com/ps/products/113/ab113687/Images/ab113687-126381-anti-pgk1-antibody-22c5d8-western-blot.jpg "Western blot - Anti-PGK1 antibody [22C5D8] (ab113687)")
Key features and details
- Mouse monoclonal [22C5D8] to PGK1
- Suitable for: WB
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
Anti-PGK1 antibody [22C5D8]
See all PGK1 primary antibodies -
Description
Mouse monoclonal [22C5D8] to PGK1 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Saccharomyces cerevisiae -
Immunogen
Full length native protein (purified). This information is considered to be commercially sensitive.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
Purity
Proprietary Purification -
Purification notes
Near homogeneity (Purity >95% by SDS-PAGE). The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by chemical fractionation. -
Clonality
Monoclonal -
Clone number
22C5D8 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab113687 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
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| Notes |
|---|
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WB
Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa). |
Target
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Function
In addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein). -
Pathway
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5. -
Involvement in disease
Defects in PGK1 are the cause of phosphoglycerate kinase 1 deficiency (PGK1D) [MIM:300653]. It is a condition with a highly variable clinical phenotype that includes hemolytic anemia, rhabdomyolysis, myopathy and neurologic involvement. Patients can express one or more of these manifestations. -
Sequence similarities
Belongs to the phosphoglycerate kinase family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 850370 Saccharomyces cerevisiae
- SwissProt: P00560 Saccharomyces cerevisiae
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Alternative names
- Cell migration-inducing gene 10 protein antibody
- Epididymis secretory sperm binding protein Li 68p antibody
- HEL S 68p antibody
see all
Images
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Western blot - Anti-PGK1 antibody [22C5D8] (ab113687)Anti-PGK1 antibody [22C5D8] (ab113687) + Yeast whole cell lysate at 15 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 45 kDa -
![Western blot - Anti-PGK1 antibody [22C5D8] (ab113687)](https://www.abcam.com/ps/products/113/ab113687/Images/ab113687-343896-anti-pgk1-antibody-22c5d8-western-blot-Saccharomyces-cerevisiae.jpg)
Western blot - Anti-PGK1 antibody [22C5D8] (ab113687)This image is courtesy of an anonymous abreview.Anti-PGK1 antibody [22C5D8] (ab113687) at 1/1000 dilution (16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk/ TBS-T) + Saccharomyces cerevisiae Cell lysate - nuclear at 30 µg
Secondary
Sheep anti-Mouse IgG, HRP-linked whole Ab ECL at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 45 kDa
Exposure time: 1 minuteMilk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C.
Gel running condition: Reduced Denaturing (4-12% Bis-Tris).
Datasheets and documents
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SDS download
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Datasheet download
References (91)
ab113687 has been referenced in 91 publications.
- Song M et al. Interplay between Pds5 and Rec8 in regulating chromosome axis length and crossover frequency. Sci Adv 7:N/A (2021). PubMed: 33712462
- Shakya VP et al. A nuclear-based quality control pathway for non-imported mitochondrial proteins. Elife 10:N/A (2021). PubMed: 33734083
- Malcova I et al. eIF3a Destabilization and TDP-43 Alter Dynamics of Heat-Induced Stress Granules. Int J Mol Sci 22:N/A (2021). PubMed: 34068231
- Edskes HK et al. Proteasome Control of [URE3] Prion Propagation by Degradation of Anti-Prion Proteins Cur1 and Btn2 in Saccharomyces cerevisiae. Genetics 218:N/A (2021). PubMed: 33742650
- Jin X et al. A Genome-Wide Screen in Saccharomyces cerevisiae Reveals a Critical Role for Oxidative Phosphorylation in Cellular Tolerance to Lithium Hexafluorophosphate. Cells 10:N/A (2021). PubMed: 33924665