Anti-PGP9.5 antibody (ab10410)
Key features and details
- Guinea pig polyclonal to PGP9.5
- Suitable for: ICC, IHC-FoFr
- Reacts with: Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-PGP9.5 antibody
See all PGP9.5 primary antibodies -
Description
Guinea pig polyclonal to PGP9.5 -
Host species
Guinea pig -
Tested applications
Suitable for: ICC, IHC-FoFrmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Macaque monkey
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Immunogen
Synthetic peptide:
GASSEDTLLKDAAKVCL
, corresponding to amino acids 175-191 of soluble cytoplasmic protein PGP9.5, human. -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: Whole serum -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab10410 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC |
1/200.
|
|
| IHC-FoFr |
1/50 - 1/500.
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| Notes |
|---|
|
ICC
1/200. |
|
IHC-FoFr
1/50 - 1/500. |
Target
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Function
Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity. -
Tissue specificity
Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients. -
Involvement in disease
Parkinson disease 5
Neurodegeneration with optic atrophy, childhood-onset -
Sequence similarities
Belongs to the peptidase C12 family. -
Post-translational
modificationsO-glycosylated. -
Cellular localization
Cytoplasm. Endoplasmic reticulum membrane. About 30% of total UCHL1 is associated with membranes in brain. - Information by UniProt
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Database links
- Entrez Gene: 7345 Human
- Entrez Gene: 29545 Rat
- Omim: 191342 Human
- SwissProt: P09936 Human
- SwissProt: Q00981 Rat
- Unigene: 518731 Human
- Unigene: 107213 Rat
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Alternative names
- Epididymis luminal protein 117 antibody
- Epididymis secretory protein Li 53 antibody
- HEL 117 antibody
see all
Images
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Immunocytochemistry - Anti-PGP9.5 antibody (ab10410)
ICC image of ab10410 stained SH-SY5Y cells. The cells were fixed with 100% methanol fixed (5 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab10410 (1/200) overnight at +4°C followed by a further incubation at room temperature for 1h with ab150185 Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 488) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown. -
Immunocytochemistry - Anti-PGP9.5 antibody (ab10410)ICC image of ab10410 stained SH-SY5Y cells. The cells were fixed with 100% methanol fixed (5 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab10410 (1/200) overnight at +4°C followed by a further incubation at room temperature for 1h with ab150187 Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 647) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown. -
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-PGP9.5 antibody - Neuronal Marker (ab10410)ab10410, at a dilution of 1/500, staining PGP9.5 in rat submucous plexus by Immunohistochemistry (PFA perfusion fixed frozen sections).
Datasheets and documents
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SDS download
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Datasheet download
References (10)
ab10410 has been referenced in 10 publications.
- Su W et al. Suppression of TLR4-MyD88 signaling pathway attenuated chronic mechanical pain in a rat model of endometriosis. J Neuroinflammation 18:65 (2021). PubMed: 33673857
- Ueha R et al. ACE2, TMPRSS2, and Furin expression in the nose and olfactory bulb in mice and humans. Rhinology 59:105-109 (2021). PubMed: 33249429
- Huang T et al. Optogenetically Controlled TrkA Activity Improves the Regenerative Capacity of Hair-Follicle-Derived Stem Cells to Differentiate into Neurons and Glia. Adv Biol (Weinh) 5:e2000134 (2021). PubMed: 32924336
- Danilova T et al. Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF) Is Highly Expressed in Mouse Tissues With Metabolic Function. Front Endocrinol (Lausanne) 10:765 (2019). PubMed: 31781038
- Dias DO et al. Reducing Pericyte-Derived Scarring Promotes Recovery after Spinal Cord Injury. Cell 173:153-165.e22 (2018). PubMed: 29502968
- Wang Z et al. Chronic constriction injury-induced microRNA-146a-5p alleviates neuropathic pain through suppression of IRAK1/TRAF6 signaling pathway. J Neuroinflammation 15:179 (2018). IHC (PFA fixed) ; Rat . PubMed: 29885668
- Chan KY et al. Engineered AAVs for efficient noninvasive gene delivery to the central and peripheral nervous systems. Nat Neurosci 20:1172-1179 (2017). IHC ; Mouse . PubMed: 28671695
- Sidorova YA et al. A Novel Small Molecule GDNF Receptor RET Agonist, BT13, Promotes Neurite Growth from Sensory Neurons in Vitro and Attenuates Experimental Neuropathy in the Rat. Front Pharmacol 8:365 (2017). IHC . PubMed: 28680400
- Yamamoto M et al. Transient receptor potential vanilloid 1-immunoreactive signals in murine enteric glial cells. World J Gastroenterol 22:9752-9764 (2016). PubMed: 27956799
- Buckinx R et al. Transient receptor potential vanilloid type 1 channel (TRPV1) immunolocalization in the murine enteric nervous system is affected by the targeted C-terminal epitope of the applied antibody. J Histochem Cytochem 61:421-32 (2013). PubMed: 23482327