Recombinant Anti-PGP9.5 antibody [EPR4117] (ab109261)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGP9.5 antibody [EPR4117] (ab109261)](https://www.abcam.com/ps/products/109/ab109261/Images/ab109261-121981-anti-pgp95-antibody-epr4117-immunohistochemistry.gif "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGP9.5 antibody [EPR4117] (ab109261)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4117] to PGP9.5
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-PGP9.5 antibody [EPR4117]
See all PGP9.5 primary antibodies -
Description
Rabbit monoclonal [EPR4117] to PGP9.5 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Fetal brain, Y79, U-87 MG, HAP1 and SH SY5Y lysates; Human brain tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 7.20 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR4117 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109261 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (2) |
1/1000 - 1/10000. Detects a band of approximately 25 kDa (predicted molecular weight: 24 kDa).
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| IHC-P | (3) |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Perform antigen retrieval before commencing with IHC staining protocol |
| Notes |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 25 kDa (predicted molecular weight: 24 kDa). |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Perform antigen retrieval before commencing with IHC staining protocol |
Target
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Function
Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity. -
Tissue specificity
Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients. -
Involvement in disease
Parkinson disease 5
Neurodegeneration with optic atrophy, childhood-onset -
Sequence similarities
Belongs to the peptidase C12 family. -
Post-translational
modificationsO-glycosylated. -
Cellular localization
Cytoplasm. Endoplasmic reticulum membrane. About 30% of total UCHL1 is associated with membranes in brain. - Information by UniProt
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Database links
- Entrez Gene: 7345 Human
- Entrez Gene: 22223 Mouse
- Entrez Gene: 29545 Rat
- Omim: 191342 Human
- SwissProt: P09936 Human
- SwissProt: Q9R0P9 Mouse
- SwissProt: Q00981 Rat
- Unigene: 518731 Human
see all -
Alternative names
- Epididymis luminal protein 117 antibody
- Epididymis secretory protein Li 53 antibody
- HEL 117 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGP9.5 antibody [EPR4117] (ab109261)
ab109261 at 1/50 dilution staining PGP9.5 in Human brain by Immunohistochemistry, Paraffin-embedded tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-PGP9.5 antibody [EPR4117] (ab109261)](https://www.abcam.com/ps/products/109/ab109261/Images/ab109261-279567-anti-pgp95-antibody-epr4117-western-blot.jpg)
Western blot - Anti-PGP9.5 antibody [EPR4117] (ab109261)Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: UCHL1 (KO) knockout HAP1 whole cell lysate (20 µg)
Lane 3: SH-SY5Y whole cell lysate (20 µg)
Lane 4: HEK293 whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab109261 observed at 24 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab109261 was shown to specifically react with UCHL1 (KO) in wild-type cells as signal was lost in UCHL1 (KO) knockout HAP1 cells. Wild-type and UCHL1 (KO) knockout samples were subjected to SDS-PAGE. ab109261 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-PGP9.5 antibody [EPR4117] (ab109261)](https://www.abcam.com/ps/products/109/ab109261/Images/ab109261-121982-anti-pgp95-antibody-epr4117-western-blot.jpg)
Western blot - Anti-PGP9.5 antibody [EPR4117] (ab109261)All lanes : Anti-PGP9.5 antibody [EPR4117] (ab109261) at 1/1000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Y79 lysate
Lane 3 : U-87 MG lysate
Lane 4 : SH SY5Y lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 24 kDa -
![Western blot - Anti-PGP9.5 antibody [EPR4117] (ab109261)](https://www.abcam.com/ps/products/109/ab109261/Images/ab109261-121980-anti-pgp95-antibody-epr4117-western-blot.jpg)
Western blot - Anti-PGP9.5 antibody [EPR4117] (ab109261)Image courtesy of Dr Simone Diestel by Abreview.All lanes : Anti-PGP9.5 antibody [EPR4117] (ab109261) at 1/1000 dilution
Lane 1 : Whole tissue lysate prepared from adult mouse brain homogenate (CD-1 strain)
Lane 2 : Whole tissue lysate prepared from non transfected control cells (low endogenous expression of UCH-L1)
Lane 3 : Whole tissue lysate prepared from cells transfected with UCH-L1-Flag cDNA (positive control)
Lysates/proteins at 100 µg per lane.
Secondary
All lanes : HRP conjugated goat anti-rabiit polyclonal at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 24 kDa
Exposure time: 2 minutes
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![OI-RD Scanning - Anti-PGP9.5 antibody [EPR4117] (ab109261)](https://www.abcam.com/ps/products/109/ab109261/Images/ab109261-121979-anti-pgp95-antibody-epr4117.jpg)
OI-RD Scanning - Anti-PGP9.5 antibody [EPR4117] (ab109261) -
![Anti-PGP9.5 antibody [EPR4117] (ab109261)](https://www.abcam.com/ps/products/109/ab109261/Images/ab109261-6-benefits-of-recombinant-antibodies.png)
Anti-PGP9.5 antibody [EPR4117] (ab109261)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab109261 has been referenced in 4 publications.
- Liu Y et al. Construction of differential expression plasmids of NGF to detect its influence on PC12 cell neuronal differentiation. Exp Ther Med 21:363 (2021). PubMed: 33732336
- Chen Y et al. Electroacupuncture at ST36 Relieves Visceral Hypersensitivity via the NGF/TrkA/TRPV1 Peripheral Afferent Pathway in a Rodent Model of Post-Inflammation Rectal Hypersensitivity. J Inflamm Res 14:325-339 (2021). PubMed: 33584100
- Huang TC et al. Thrombomodulin facilitates peripheral nerve regeneration through regulating M1/M2 switching. J Neuroinflammation 17:240 (2020). PubMed: 32799887
- Purmessur D et al. Notochordal cell-derived therapeutic strategies for discogenic back pain. Global Spine J 3:201-18 (2013). IHC . PubMed: 24436871