Recombinant Anti-PHF1 antibody [EPR14222] (ab184951)

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Western blot - Anti-PHF1 antibody [EPR14222] (ab184951)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR14222] to PHF1
Suitable for: Flow Cyt (Intra), IHC-P, ICC/IF, WB, IP
Reacts with: Mouse, Rat, Human

APC Carrier Free HRP PE

Product name

Anti-PHF1 antibody [EPR14222]
See all PHF1 primary antibodies
Description

Rabbit monoclonal [EPR14222] to PHF1
Host species

Rabbit
Specificity

This antibody is specific to PHD finger protein 1, not paired helical filaments.
Tested applications

Suitable for: Flow Cyt (Intra), IHC-P, ICC/IF, WB, IPmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- 293, Jurkat, MCF7 and Human fetal brain lysates; Human pancreas and Rat skeletal muscle tissues; MCF7 cells; 293 cells. Mouse and rat brain tissue lysate. NIH/3T3, RAW264.7, C6 and PC-12 whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR14222
Isotype

IgG
Research areas

Alternative Versions
- Anti-PHF1 antibody [EPR14222] - BSA and Azide free (ab232601)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab184951 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
Flow Cyt (Intra)		1/160. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P		1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF		1/250 - 1/500.
WB		1/1000 - 1/10000. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).
IP		1/30.

Notes
Flow Cyt (Intra) 1/160. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/250 - 1/500.
WB 1/1000 - 1/10000. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).
IP 1/30.

Function

Transcriptional repressor. May promote methylation of histone H3 on 'Lys-27' by the PRC2/EED-EZH2 complex.
Tissue specificity

Highest levels in heart, skeletal muscle, and pancreas, lower levels in brain, placenta, lung, liver and kidney.
Sequence similarities

Contains 2 PHD-type zinc fingers.
Cellular localization

Nucleus. Localizes specifically to the promoters of numerous target genes.
Target information above from: UniProt accession O43189 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 5252 Human
- Entrez Gene: 21652 Mouse
- Entrez Gene: 294287 Rat
- Omim: 602881 Human
- SwissProt: O43189 Human
- SwissProt: Q9Z1B8 Mouse
- Unigene: 166204 Human
- Unigene: 480590 Mouse
Alternative names
- hPCl1 antibody
- MTF2 L2 antibody
- MTF2L 2 antibody
- MTF2L2 antibody
- PCL 1 antibody
- PCL1 antibody
- PHD finger protein 1 antibody
- PHD finger protein1 antibody
- PHF 1 antibody
- PHF 2 antibody
- Phf1 antibody
- PHF1_HUMAN antibody
- PHF2 antibody
- Plc 1 antibody
- Plc1 antibody
- Polycomb like protein 1 antibody
- Polycomb like protein1 antibody
- Polycomb-like protein 1 antibody
- Protein PHF1 antibody
- T complex testis expressed 3 antibody
- T complex testis expressed3 antibody
- Tctex 3 antibody
- Tctex3 antibody
see all

Western blot - Anti-PHF1 antibody [EPR14222] (ab184951)

All lanes : Anti-PHF1 antibody [EPR14222] (ab184951) at 1/1000 dilution

Lane 1 : Mouse brain tissue
Lane 2 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lane 3 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lane 4 : Rat brain tissue lysate
Lane 5 : C6 (rat glial tumor glial cell) whole cell lysate
Lane 6 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

Predicted band size: 62 kDa
Immunoprecipitation - Anti-PHF1 antibody [EPR14222] (ab184951)

PHF1 was immunoprecipitated from 0.35 mg Mouse brain tissue lysate 10 ug with ab184951 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab184951 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1: Mouse brain tissue lysate 10 ug

Lane 2: ab184951 IP in Mouse brain tissue lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab184951 in mouse brain tissue lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 minutes
Western blot - Anti-PHF1 antibody [EPR14222] (ab184951)

All lanes : Anti-PHF1 antibody [EPR14222] (ab184951) at 1/20000 dilution

Lane 1 : 293 lysate
Lane 2 : Jurkat lysate
Lane 3 : MCF7 lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 62 kDa
Western blot - Anti-PHF1 antibody [EPR14222] (ab184951)

Anti-PHF1 antibody [EPR14222] (ab184951) at 1/5000 dilution + Human fetal brain lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 62 kDa
Immunocytochemistry/ Immunofluorescence - Anti-PHF1 antibody [EPR14222] (ab184951)

Immunofluorescent analysis of MCF7 cells (4% paraformaldehyde-fixed) labeling PHF1 with ab184951 at 1/500 dilution followed by Goat anti rabbit IgG (AlexaFluor® 555) secondary at 1/200 dilution and counter-stained with DAPI (blue).
Flow Cytometry (Intracellular) - Anti-PHF1 antibody [EPR14222] (ab184951)

Intracellular flow cytometric analysis of 293 cells (2% paraformaldehyde-fixed) labeling PHF1with ab184951 at 1/160 dilution (red) mpared to a negative control antibody (red)or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHF1 antibody [EPR14222] (ab184951)

Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling PHF1 with ab184951 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHF1 antibody [EPR14222] (ab184951)

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling PHF1 with ab184951 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Anti-PHF1 antibody [EPR14222] (ab184951)

Flow cytometry protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab184951? Please let us know so that we can cite the reference in this datasheet.

ab184951 has been referenced in 6 publications.

Yu CC et al. Preventive electroacupuncture reduces cognitive deficits in a rat model of D-galactose-induced aging. Neural Regen Res 16:916-923 (2021). PubMed: 33229729
Zhao J et al. APOE4 exacerbates synapse loss and neurodegeneration in Alzheimer's disease patient iPSC-derived cerebral organoids. Nat Commun 11:5540 (2020). PubMed: 33139712
Alexandrova E et al. Interaction Proteomics Identifies ERbeta Association with Chromatin Repressive Complexes to Inhibit Cholesterol Biosynthesis and Exert An Oncosuppressive Role in Triple-negative Breast Cancer. Mol Cell Proteomics 19:245-260 (2020). PubMed: 31792072
Liu H et al. Guanosine monophosphate reductase 1 is a potential therapeutic target for Alzheimer's disease. Sci Rep 8:2759 (2018). PubMed: 29426890
Qu Y et al. PHF1 is required for chromosome alignment and asymmetric division during mouse meiotic oocyte maturation. Cell Cycle 17:2447-2459 (2018). PubMed: 30382790
Noël A et al. Sex-specific lung functional changes in adult mice exposed only to second-hand smoke in utero. Respir Res 18:104 (2017). WB ; Mouse . PubMed: 28651580

Customer reviews and Q&As

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Application

Immunohistochemistry (Frozen sections)

Sample

Mouse Tissue sections (Testis)

Permeabilization

Yes - 0.1% Tween

Specification

Testis

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 07 2018

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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Specificity

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Isotype control

Positive Controls

Applications

The Abpromise guarantee

Target

Function

Tissue specificity

Sequence similarities

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

Certificate of Compliance

References (6)

Customer reviews and Q&As

Immunohistochemistry (Frozen sections) abreview for Anti-PHF1 antibody [EPR14222]