Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
RecombinantRabMAb

Recombinant Anti-PHLPP2 antibody [SP220] (ab227673)

Submit a review Submit a question References (1)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHLPP2 antibody [SP220] (ab227673)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHLPP2 antibody [SP220] (ab227673)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHLPP2 antibody [SP220] (ab227673)
  • Western blot - Anti-PHLPP2 antibody [SP220] (ab227673)
  • Anti-PHLPP2 antibody [SP220] (ab227673)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP220] to PHLPP2
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-PHLPP2 antibody [SP220]
    See all PHLPP2 primary antibodies

  • Description

    Rabbit monoclonal [SP220] to PHLPP2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human prostate, and prostate carcinoma tissue; WB: HEK293T cell lysate.

  • General notes

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab227673 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/400. Predicted molecular weight: 147 kDa.

Primary antibody incubation for 1 hour at room temperature.
IHC-P
1/100.

Perform heat mediated antigen retrieval by boiling tissue section in citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes.

Primary antibody incubation for 10 minutes at room temperature.
Notes
WB
1/400. Predicted molecular weight: 147 kDa.

Primary antibody incubation for 1 hour at room temperature.
IHC-P
1/100.

Perform heat mediated antigen retrieval by boiling tissue section in citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes.

Primary antibody incubation for 10 minutes at room temperature.

Target

  • Function

    Protein phosphatase that mediates dephosphorylation of 'Ser-473' of AKT1, 'Ser-660' of PRKCB isoform beta-II and 'Ser-657' of PRKCA. AKT1 regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of AKT1 triggers apoptosis and decreases cell proliferation. Also controls the phosphorylation of AKT3. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation. Inhibits cancer cell proliferation and may act as a tumor suppressor.

  • Tissue specificity

    In colorectal cancer tissue, expression is highest in the surface epithelium of normal colonic mucosa adjacent to the cancer tissue but is largely excluded from the crypt bases. Expression is lost or significantly decreased in 80% of tested tumors (at protein level).

  • Sequence similarities

    Contains 22 LRR (leucine-rich) repeats.
    Contains 1 PH domain.
    Contains 1 PP2C-like domain.

  • Cellular localization

    Cytoplasm. Membrane. Nucleus. In colorectal cancer tissue, expression is concentrated in the cytoplasm and nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • KIAA0931 antibody
      • PH domain and leucine rich repeat protein phosphatase 2 antibody
      • PH domain leucine rich repeat protein phosphatase like antibody
      • PH domain leucine-rich repeat-containing protein phosphatase 2 antibody
      • PH domain leucine-rich repeat-containing protein phosphatase-like antibody
      • PHLP2_HUMAN antibody
      • PHLPP like antibody
      • PHLPP-like antibody
      • Phlpp2 antibody
      • PHLPPL antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHLPP2 antibody [SP220] (ab227673)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHLPP2 antibody [SP220] (ab227673)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human prostate carcinoma tissue sections labeling PHLPP2 with ab227673 at 1/100 dilution (1.65 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHLPP2 antibody [SP220] (ab227673)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHLPP2 antibody [SP220] (ab227673)

      Formalin-fixed, paraffin-embedded human prostate tissue stained for PHLPP2 using ab227673 at 1/100 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHLPP2 antibody [SP220] (ab227673)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHLPP2 antibody [SP220] (ab227673)

      Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for PHLPP2 using ab227673 at 1/100 dilution in immunohistochemical analysis.

    • Western blot - Anti-PHLPP2 antibody [SP220] (ab227673)
      Western blot - Anti-PHLPP2 antibody [SP220] (ab227673)
      Anti-PHLPP2 antibody [SP220] (ab227673) at 1/400 dilution + HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate.

      Predicted band size: 147 kDa

    • Anti-PHLPP2 antibody [SP220] (ab227673)
      Anti-PHLPP2 antibody [SP220] (ab227673)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (1)

    Publishing research using ab227673? Please let us know so that we can cite the reference in this datasheet.

    ab227673 has been referenced in 1 publication.

    • Wang M  et al. LncRNA LOC729178 acts as a sponge of miR-144-3p to mitigate cigarette smoke extract-induced inflammatory injury via regulating PHLPP2 in 16HBE cells. J Mol Histol 52:437-447 (2021). PubMed: 33847879

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab227673.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com