Anti-PHO8 antibody [1D3A10] (ab113688)
![Western blot - Anti-PHO8 antibody [1D3A10] (ab113688)](https://www.abcam.com/ps/products/113/ab113688/Images/ab113688-126382-anti-pho8-antibody-1d3a10-western-blot.jpg "Western blot - Anti-PHO8 antibody [1D3A10] (ab113688)")
Key features and details
- Mouse monoclonal [1D3A10] to PHO8
- Suitable for: WB, ICC/IF
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG1
Overview
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Product name
Anti-PHO8 antibody [1D3A10] -
Description
Mouse monoclonal [1D3A10] to PHO8 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Saccharomyces cerevisiae -
Immunogen
Full length native protein (purified). This information is considered to be commercially sensitive.
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Positive control
- Yeast lysate
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
Purity
Proprietary Purification -
Purification notes
Near homogeneity (Purity >95% by SDS-PAGE). The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by chemical fractionation. -
Clonality
Monoclonal -
Clone number
1D3A10 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab113688 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 4 µg/ml. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).
|
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| ICC/IF |
Use a concentration of 20 µg/ml.
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| Notes |
|---|
|
WB
Use a concentration of 4 µg/ml. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa). |
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ICC/IF
Use a concentration of 20 µg/ml. |
Target
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Function
Phosphatase with broad substrate specificity. A truncated (soluble) version of the protein is responsible for the production of (E,E)-farnesol from (E,E)-farnesyl diphosphate. -
Sequence similarities
Belongs to the alkaline phosphatase family. -
Cellular localization
Cytoplasm. The truncated version of the protein is soluble and Vacuole membrane. The full-length version is found in lysosome-like vacuoles. - Information by UniProt
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Database links
- Entrez Gene: 852092 Saccharomyces cerevisiae
- SwissProt: P11491 Saccharomyces cerevisiae
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Alternative names
- Farnesyl diphosphatase antibody
- Membrane-bound repressible alkaline phosphatase antibody
- PHO8 antibody
see all
Images
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Western blot - Anti-PHO8 antibody [1D3A10] (ab113688)Anti-PHO8 antibody [1D3A10] (ab113688) + Yeast whole cell lysate at 15 µg
Predicted band size: 63 kDa
Protocols
Datasheets and documents
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Datasheet download
References (8)
ab113688 has been referenced in 8 publications.
- Makino S et al. Selectivity of mRNA degradation by autophagy in yeast. Nat Commun 12:2316 (2021). PubMed: 33875662
- Corradi GR et al. Reduction of the P5A-ATPase Spf1p phosphoenzyme by a Ca2+-dependent phosphatase. PLoS One 15:e0232476 (2020). PubMed: 32353073
- Nguyen TQ et al. Isolated Saccharomyces cerevisiae vacuoles contain low-molecular-mass transition-metal polyphosphate complexes. Metallomics 11:1298-1309 (2019). PubMed: 31210222
- McNally EK & Brett CL The intralumenal fragment pathway mediates ESCRT-independent surface transporter down-regulation. Nat Commun 9:5358 (2018). PubMed: 30560896
- Szoradi T et al. SHRED Is a Regulatory Cascade that Reprograms Ubr1 Substrate Specificity for Enhanced Protein Quality Control during Stress. Mol Cell 70:1025-1037.e5 (2018). PubMed: 29861160
- Smindak RJ et al. Lipid-dependent regulation of exocytosis in S. cerevisiae by OSBP homolog (Osh) 4. J Cell Sci 130:3891-3906 (2017). PubMed: 28993464
- Manandhar SP et al. Saccharomyces cerevisiae Env7 is a novel serine/threonine kinase 16-related protein kinase and negatively regulates organelle fusion at the lysosomal vacuole. Mol Cell Biol 33:526-42 (2013). PubMed: 23166297
- Beach A et al. Mitochondrial membrane lipidome defines yeast longevity. Aging (Albany NY) 5:551-74 (2013). PubMed: 23924582
