Anti-Phospho - (Ser/Thr) Phe antibody (ab17464)
Key features and details
- Rabbit polyclonal to Phospho - (Ser/Thr) Phe
- Suitable for: ELISA, IP, WB, ICC/IF
- Reacts with: Species independent
- Isotype: IgG
Overview
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Product name
Anti-Phospho - (Ser/Thr) Phe antibody
See all Phospho - (Ser/Thr) Phe primary antibodies -
Description
Rabbit polyclonal to Phospho - (Ser/Thr) Phe -
Host species
Rabbit -
Tested applications
Suitable for: ELISA, IP, WB, ICC/IFmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Synthetic peptide corresponding to Phospho - (Ser/Thr) Phe . Immunogen = Synthetic phospho-serine/threonine-phenylalanine containing peptide.
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General notes
The immunogen used is specific phosphorylated serine or threonine amino acids surrounded by phenylalanine. (Y/F/W)(S*/T*)F
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.50
Constituents: 0.238% HEPES, 50% Glycerol, 0.87% Sodium chloride, 0.01% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified by Protein A and peptide affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab17464 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
1/500.
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IP |
1/100.
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WB | (5) |
1/1000.
Block with 5% nonfat milk, 0.1% Tween-20. Dilute primary antibody in 5% BSA, 0.1% Tween-20. |
ICC/IF | (1) |
Use at an assay dependent concentration.
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Notes |
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ELISA
1/500. |
IP
1/100. |
WB
1/1000. Block with 5% nonfat milk, 0.1% Tween-20. Dilute primary antibody in 5% BSA, 0.1% Tween-20. |
ICC/IF
Use at an assay dependent concentration. |
Images
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Western blot - Anti-Phospho - (Ser/Thr) Phe antibody (ab17464)Image from Gao X et al, PLoS Pathog 5:e1000708 (2009), Fig S3.To determine if NleH could phosphorylate RPS3, we conducted in vitro kinase assays with NleH and RPR3 and analyzed the results by Immunoblotting with ab17464, phospho-Ser/Thr-specific antibody, following separation by SDS-PAGE.
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Western blot - Anti-Phospho - (Ser/Thr) Phe antibody (ab17464)This image is courtesy of an anonymous AbreviewAll lanes : Anti-Phospho - (Ser/Thr) Phe antibody (ab17464) at 1/1000 dilution
Lane 1 : Human THP-1 Whole Cell Extract
Lane 2 : Mouse Total Lung Extract
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Alkaline phosphatase conjugated goat anti-rabbit antibody
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 5 minutes
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All lanes : Anti-Phospho - (Ser/Thr) Phe antibody (ab17464) at 1/1000 dilution
All lanes : calyculin A treated A431 cells
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (94)
ab17464 has been referenced in 94 publications.
- Kanwar P et al. CIPK9 targets VDAC3 and modulates oxidative stress responses in Arabidopsis. Plant J 109:241-260 (2022). PubMed: 34748255
- Yuan Y et al. High salt activates p97 to reduce host antiviral immunity by restricting Viperin induction. EMBO Rep 23:e53466 (2022). PubMed: 34779558
- Ren BX et al. The antibiotic drug trimethoprim suppresses tumour growth and metastasis via targeting Snail. Br J Pharmacol 179:2659-2677 (2022). PubMed: 34855201
- Wang L et al. OsMAPK6 phosphorylates a zinc finger protein OsLIC to promote downstream OsWRKY30 for rice resistance to bacterial blight and leaf streak. J Integr Plant Biol 64:1116-1130 (2022). PubMed: 35293133
- Yu B et al. KMT5A-methylated SNIP1 promotes triple-negative breast cancer metastasis by activating YAP signaling. Nat Commun 13:2192 (2022). PubMed: 35449131