Recombinant Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EP1898-7Y] (ab76155)
![Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EP1898-7Y] (ab76155)](https://www.abcam.com/ps/products/76/ab76155/Images/ab76155-259097-ab76155KOWB.jpg "Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EP1898-7Y] (ab76155)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1898-7Y] to Phospholipase C gamma 1/PLC-gamma-1
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EP1898-7Y]
See all Phospholipase C gamma 1/PLC-gamma-1 primary antibodies -
Description
Rabbit monoclonal [EP1898-7Y] to Phospholipase C gamma 1/PLC-gamma-1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
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Immunogen
Synthetic peptide within Human Phospholipase C gamma 1/PLC-gamma-1. The exact sequence is proprietary.
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Positive control
- Jurkat whole cell lysate (ab7899).
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1898-7Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Antibody Diluent
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab76155 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/5000 - 1/10000. Detects a band of approximately 149 kDa (predicted molecular weight: 149 kDa).
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| Notes |
|---|
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WB
1/5000 - 1/10000. Detects a band of approximately 149 kDa (predicted molecular weight: 149 kDa). |
Target
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Function
Plays a role in actin reorganization and cell migration. The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. -
Sequence similarities
Contains 1 C2 domain.
Contains 1 EF-hand domain.
Contains 2 PH domains.
Contains 1 PI-PLC X-box domain.
Contains 1 PI-PLC Y-box domain.
Contains 2 SH2 domains.
Contains 1 SH3 domain. -
Domain
The SH3 domain mediates interaction with CLNK (By similarity). The SH3 domain also mediates interaction with RALGPS1. -
Post-translational
modificationsThe receptor-mediated activation of PLC-gamma-1 and PLC-gamma-2 involves their phosphorylation by tyrosine kinases in response to ligation of a variety of growth factor receptors and immune system receptors. May be dephosphorylated by PTPRJ.
Ubiquitinated by CBLB in activated T-cells. -
Cellular localization
Cell projection > lamellipodium. Cell projection > ruffle. Rapidly redistributed to ruffles and lamellipodia structures in response to epidermal growth factor (EGF) treatment. - Information by UniProt
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Database links
- Entrez Gene: 5335 Human
- Entrez Gene: 18803 Mouse
- Entrez Gene: 25738 Rat
- Omim: 172420 Human
- SwissProt: P19174 Human
- SwissProt: Q62077 Mouse
- SwissProt: P10686 Rat
- Unigene: 268177 Human
see all -
Alternative names
- 1 phosphatidyl D myo inositol 4 5 bisphosphate antibody
- 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 1 antibody
- 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1 antibody
see all
Images
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Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EP1898-7Y] (ab76155)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Phospholipase C gamma 1/PLC-gamma-1 knockout HAP1 cell lysate (20 µg)
Lane 3: HepG2 cell lysate (20 µg)
Lane 4: Mouse brain tissue lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab76155 observed at 160 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab76155 was shown to specifically react with Phospholipase C gamma 1/PLC-gamma-1 when Phospholipase C gamma 1/PLC-gamma-1 knockout samples were used. Wild-type and Phospholipase C gamma 1/PLC-gamma-1 knockout samples were subjected to SDS-PAGE. ab76155 at a dilution of 1/5000 and ab8245 (loading control to GAPDH) at a dilution of 1/10,000 were incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging. -
![Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EP1898-7Y] (ab76155)](https://www.abcam.com/ps/products/76/ab76155/Images/ab76155wb.gif)
Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EP1898-7Y] (ab76155)Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EP1898-7Y] (ab76155) at 1/10000 dilution + Jurkat cell lysates at 10 µg
Secondary
goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 149 kDa
Observed band size: 149 kDa -
![Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EP1898-7Y] (ab76155)](https://www.abcam.com/ps/products/76/ab76155/Images/ab76155-4-benefits-of-recombinant-antibodies.png)
Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EP1898-7Y] (ab76155)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab76155 has been referenced in 8 publications.
- Fu P et al. Aerobic exercise promotes the functions of brown adipose tissue in obese mice via a mechanism involving COX2 in the VEGF signaling pathway. Nutr Metab (Lond) 18:56 (2021). PubMed: 34082784
- Liu H et al. A novel multikinase inhibitor SKLB-YTH-60 ameliorates inflammation and fibrosis in bleomycin-induced lung fibrosis mouse models. Cell Prolif 54:e13081 (2021). PubMed: 34121240
- Ma T et al. Activation of brain-derived neurotrophic factor signaling in the basal forebrain reverses acute sleep deprivation-induced fear memory impairments. Brain Behav 10:e01592 (2020). PubMed: 32157827
- Ma JX et al. Couplet medicines of leech and centipede granules improve erectile dysfunction via inactivation of the CaSR/PLC/PKC signaling in streptozotocin-induced diabetic rats. Biosci Rep 40:N/A (2020). PubMed: 31922200
- Ma ZG et al. Toll-like receptor 5 deficiency diminishes doxorubicin-induced acute cardiotoxicity in mice. Theranostics 10:11013-11025 (2020). PubMed: 33042267
- Francis C et al. Ferric citrate reduces fibroblast growth factor 23 levels and improves renal and cardiac function in a mouse model of chronic kidney disease. Kidney Int 96:1346-1358 (2019). PubMed: 31668632
- Wang LN et al. A role for GPI-CD59 in promoting T-cell signal transduction via LAT. Oncol Lett 15:4873-4881 (2018). PubMed: 29541246
- Sikorski K et al. A high-throughput pipeline for validation of antibodies. Nat Methods 15:909-912 (2018). PubMed: 30377371